Transient apoptosis inhibition in donor cells increases efficacy of hematopoietic stem cell transplantation without increasing the risk of leukemogenesis

Kollek, Matthias; Voigt, G; Bertele, Daniela; Garcia, Anne; Maurer, Ulrich; Krombholz, F; Labi, Verena; Fischer, Nicolas; Niemeyer, Charlotte M.; Geley, Stephan; Villunger, Andreas; Erlacher, Miriam

doi:10.1055/s-0034-1374838

Cited by 1 publication

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“…Given that only a very small portion of systematically injected MSCs that can normally lodge in the target tissue 36 , our preliminary finding, i.e., considerable number of injected AnSCs lodged in the healing tissue, may be novel and provide another explanation why AnSCs had marginal advantage over the other types of MSCs used in wound healing of this study. Although the number of lodged AnSCs was significantly reduced from day 7 to day 14 after cell transplantation and majority of the homing AnSCs did not persist in the target tissue, likely via apoptotic pathway 37 . Substances released by the dying cells may also play an important role in stimulating wound healing.…”

Section: Anscs Lineage Tracingmentioning

confidence: 96%

Transplanted Antler Stem Cells Stimulated Regenerative Healing of Radiation-induced Cutaneous Wounds in Rats

Rong

Zhang²,

Yang

et al. 2020

Cell Transplant

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Radiation-induced cutaneous injury is the main side effect of radiotherapy. The injury is difficult to cure and the pathogenesis is complex. Mesenchymal stem cells (MSCs) serve as a promising candidate for cell-based therapy for the treatment of cutaneous wounds. The aim of the present study was to investigate whether antler stem cells (AnSCs) have better therapeutic effects on radiation-induced cutaneous injury than currently available ones. In this study, a rat model of cutaneous wound injury from Sr-90 radiation was used. AnSCs (1 × 106/500 μl) were injected through the tail vein on the first day of irradiation. Our results showed that compared to the control group, AnSC-treated rats exhibited a delayed onset (14 days versus 7 days), shorter recovery time (51 days versus 84 days), faster healing rate (100% versus 70% on day 71), and higher healing quality with more cutaneous appendages regenerated (21:10:7/per given area compared to those of rat and human MSCs, respectively). More importantly, AnSCs promoted much higher quality of healing compared to other types of stem cells, with negligible scar formation. AnSC lineage tracing results showed that the injected-dye-stained AnSCs were substantially engrafted in the wound healing tissue, indicating that the therapeutic effects of AnSCs on wound healing at least partially through direct participation in the wound healing. Expression profiling of the wound-healing-related genes in the healing tissue of AnSC group more resembled a fetal wound healing. Revealing the mechanism underlying this higher quality of wound healing by using AnSC treatment would help to devise more effective cell-based therapeutics for radiation-induced wound healing in clinics.

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Section: Anscs Lineage Tracingmentioning

confidence: 96%