Transient Absorption of Chlorophylls and Carotenoids after Two-Photon Excitation of LHCII

Šebelík, Václav; Kuznetsova, Valentyna; Lokstein, Heiko; Polı́vka, Tomáš

doi:10.1021/acs.jpclett.1c00122

Cited by 14 publications

(9 citation statements)

References 47 publications

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“…Drought stress affects the photochemical reactions of the leaves, triggering photoinhibition that will result in more light energy being absorbed by the leaves than the plant can use. Therefore, the excess light energy causes photooxidative stress [32][33][34][35][36].…”

Section: Discussionmentioning

confidence: 99%

Relationship among Electrical Signals, Chlorophyll Fluorescence, and Root Vitality of Strawberry Seedlings under Drought Stress

Zhou

Yuan

et al. 2022

Agronomy

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Drought area expansion has a great impact on the growth and development of plants. To contribute to the water management of strawberry, this work aims to study the chronological relationship between the electrical signals and representative physiological parameters of strawberry seedlings under drought stress. This study analyzed the characteristic variables of the electrical signals; physiological parameters under drought; and control treatments. Moreover, we compared the chronological sequence of the appearance of significant differences between drought and control treatment in terms of their physiological parameters and electrical signals. The results showed that with the increase of drought treatment, the time domain parameters (peak-to-peak value, standard deviation) and frequency domain parameters (spectral of central gravity, power spectrum entropy) of the drought-treated electrical signals showed significant differences from the control on Day 2 and Day 6, respectively (p < 0.05). The root vitality of the drought treatment was significantly different from the control on Day 4 (p < 0.05); the Fv/Fm and the SPAD were significantly different (p < 0.05) on Day 7. Electrical signals first start to show a significant difference between drought and control treatment, followed by physiological parameters. Therefore, the electrical signal can be used as an early indicator of drought stress conditions. This will provide a scientific basis for the actual water management of strawberry seedlings. It also provides a methodological and theoretical basis for other studies analyzing the relationship between plant physiological parameters and electrical signals under other stress conditions.

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Section: Discussionmentioning

confidence: 99%

Relationship among Electrical Signals, Chlorophyll Fluorescence, and Root Vitality of Strawberry Seedlings under Drought Stress

Zhou

Yuan

et al. 2022

Agronomy

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“…A brand-new challenge for the theoretical approaches was set by the femtosecond time-resolved two-photon excitation (2PE) experiments. − Due to its multielectron character, the S 0 -S 1 transition is one-photon forbidden, but two-photon allowed. Considering that two-photon excitation does not populate the S 2 , the 2PE transient absorption spectra can be viewed as the “pure” S 1 state, minimizing artifacts arising from the depopulation of the higher lying energy levels.…”

Section: Perspective: Vera On Two-photon Initiated Ultrafast Dynamicsmentioning

confidence: 99%

Understanding Carotenoid Dynamics via the Vibronic Energy Relaxation Approach

et al. 2022

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Carotenoids are an integral part of natural photosynthetic complexes, with tasks ranging from light harvesting to photoprotection. Their underlying energy deactivation network of optically dark and bright excited states is extremely efficient: after excitation of light with up to 2.5 eV of photon energy, the system relaxes back to ground state on a time scale of a few picoseconds. In this article, we summarize how a model based on the vibrational energy relaxation approach (VERA) explains the main characteristics of relaxation dynamics after one-photon excitation with special emphasis on the so-called S* state. Lineshapes after two-photon excitation are beyond the current model of VERA. We outline this future line of research in our article. In terms of experimental method development, we discuss which techniques are needed to better describe energy dissipation effects in carotenoids and within the first solvation shell.

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“…Hence, a re-evaluation of previously obtained data appears to be required. This endeavor is currently underway in our labs [ 160 , 161 ]. However, data obtained with NLPF comparing trimeric and (slightly) aggregated LHCII seemed to corroborate a change of Chl-xanthophyll interactions to be the origin of NPQ [ 43 ].…”

Section: Photodamage Photoprotection and Regulation Of Functional Antenna Sizementioning

confidence: 99%

Photosynthetic Light-Harvesting (Antenna) Complexes—Structures and Functions

2021

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Chlorophylls and bacteriochlorophylls, together with carotenoids, serve, noncovalently bound to specific apoproteins, as principal light-harvesting and energy-transforming pigments in photosynthetic organisms. In recent years, enormous progress has been achieved in the elucidation of structures and functions of light-harvesting (antenna) complexes, photosynthetic reaction centers and even entire photosystems. It is becoming increasingly clear that light-harvesting complexes not only serve to enlarge the absorption cross sections of the respective reaction centers but are vitally important in short- and long-term adaptation of the photosynthetic apparatus and regulation of the energy-transforming processes in response to external and internal conditions. Thus, the wide variety of structural diversity in photosynthetic antenna “designs” becomes conceivable. It is, however, common for LHCs to form trimeric (or multiples thereof) structures. We propose a simple, tentative explanation of the trimer issue, based on the 2D world created by photosynthetic membrane systems.

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Transient Absorption of Chlorophylls and Carotenoids after Two-Photon Excitation of LHCII

Cited by 14 publications

References 47 publications

Relationship among Electrical Signals, Chlorophyll Fluorescence, and Root Vitality of Strawberry Seedlings under Drought Stress

Relationship among Electrical Signals, Chlorophyll Fluorescence, and Root Vitality of Strawberry Seedlings under Drought Stress

Understanding Carotenoid Dynamics via the Vibronic Energy Relaxation Approach

Photosynthetic Light-Harvesting (Antenna) Complexes—Structures and Functions

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