Transglutaminase-induced crosslinking of gelatin–calcium carbonate composite films

Wang, Yuemeng; Liu, Anjun; Ye, Ran; Wang, Wenhang; Li, Xin

doi:10.1016/j.foodchem.2014.06.062

Cited by 130 publications

(100 citation statements)

References 42 publications

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“…1 mg of the sample was mixed with 150 mg of KBr and pressed into a pellet according to the published method (Chen, Ye, & Liu, 2014;Wang, Liu, Ye, Wang, & Li, 2015). FTIR spectra were recorded at the absorbance from 4000 cm À1 to 400 cm À1 with a Thermo Nicolet Avatar 370 Fourier Transform Infrared spectrometer equipped with a DTGS KBr detector (Thermo, Shanghai, China).…”

Section: Fourier Transform Infrared Spectroscopy (Ftir)mentioning

confidence: 99%

Blasting extrusion processing: The increase of soluble dietary fiber content and extraction of soluble-fiber polysaccharides from wheat bran

2015

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Section: Fourier Transform Infrared Spectroscopy (Ftir)mentioning

confidence: 99%

Blasting extrusion processing: The increase of soluble dietary fiber content and extraction of soluble-fiber polysaccharides from wheat bran

2015

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“…Nevertheless, the inferior strength and poor water barrier impede its commercialization in food applications. Although the mechanical properties of geltain films was improved by some studies (Lin et al., ; Staroszczyk et al., ; Y. Wang, Liu, Ye, Wang, & Li, ), the lower moisture barriers of gelatin film is still a big challenge, due to the gelatin's hydrophilicity (Tongnuanchan, Benjakul, Prodpran, & Nilsuwan, ). So, adding hydrophobic substances into gelatin matrix has been performed in some studies (Limpisophon, Tanaka, & Osako, ; Tongnuanchan et al., ).…”

Section: Introductionmentioning

confidence: 99%

An Attempt of Using β‐Sitosterol‐Corn Oil Oleogels to Improve Water Barrier Properties of Gelatin Film

Xiao

Zhang

Wang

et al. 2019

Journal of Food Science

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Oleogel with tailored viscoelasticity is a great interest for food structuring, while its potential benefits for edible film performance are not clear. In this study, β‐sitosterol (0, 5, 10, 15, and 20 wt%)‐corn oil oleogel was developed and used in the formation of gelatin‐based films. Importantly, adding oleogel significantly decreased water vapor permeability of the gelatin films, however, it had little negative influence on film strength. In addition, the results of this study demonstrated that increasing the sitosterol in oleogels led to an increasing number of ordered crystals formed in the oleogel, which contributed to compact and smooth surface of the film. Moreover, the incorporation of oleogel also caused some changes in molecule conformation and film barrier property. Therefore, the superior mechanical performance and moisture resistance properties of the film were obtained when 15% β‐sitosterol was used to prepare oleogel. Practical Application Corn oil oleogels β‐sitosterol was incorporated with gelatin to prepare the gelatin film aiming to improve the water resistance of the films for its variety of practical production. The enhanced vapor permeability and accepted strength of the emulsion film indicated the potential application of it with a variety of edible packaging forms, such as films, pouch and sachet in medium and high humid condition.

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“…Microbial transglutaminase works nearly as effectively as mammalian transglutaminase in crosslinking of gelatin molecules via the formation of a covalent bond between ε-amino groups from lysine residues and γ-carboxamide groups of glutamine of gelatin molecules. 22,23 Then, characterization of the homogeneity of the crosslinked film was performed by confocal microscopy (CLSM). 21 A first characterization of the crosslinking of the film was performed with FT-IR (Fig.…”

Section: Build Up Of the Spin-coated Filmmentioning

confidence: 99%

Priming cells for their final destination: microenvironment controlled cell culture by a modular ECM-mimicking feeder film

et al. 2015

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Mammalian cell culture is the starting point in many research studies focusing on biomedical applications. However, researchers have little control over the standardized cell microenvironment parameters. Here a modular ECM-mimicking surface coating for cell culture environment is designed. This substrate is a new and versatile thin film obtained by spin-coating of concentrated gelatin crosslinked by transglutaminase. It can be modified with respect to the biochemical and biophysical needs of the final cell destination, i.e. it delivers loaded multi-growth factors and serum components and allows for cell culture in a serum-free culture medium. Also, a well-known cell behavior modulator, the substrate stiffness, is controlled exogenously by addition of nanoparticles. In addition to growth factors, antimicrobial agents such as natural peptides are added to the substrate for limiting the repeated addition of antimicrobial agents to the culture medium and to prevent the increase of resistant bacterial strains in the culture environment. Finally, this substrate contains simultaneously ECM components, growth factors, stiffening elements and antimicrobial agents. It provides a favorable microenvironment and sterile conditions. It is a free-of-maintenance system, as cells will grow without addition of serum or antimicrobial cocktails. This low cost and easy-to-use substrate could emerge as a new standard for cell culture.

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Transglutaminase-induced crosslinking of gelatin–calcium carbonate composite films

Cited by 130 publications

References 42 publications

Blasting extrusion processing: The increase of soluble dietary fiber content and extraction of soluble-fiber polysaccharides from wheat bran

Blasting extrusion processing: The increase of soluble dietary fiber content and extraction of soluble-fiber polysaccharides from wheat bran

An Attempt of Using β‐Sitosterol‐Corn Oil Oleogels to Improve Water Barrier Properties of Gelatin Film

Priming cells for their final destination: microenvironment controlled cell culture by a modular ECM-mimicking feeder film

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