Transglutaminase activity and putrescine‐binding capacity in cloned cell lines with different metastatic potential

Abstract

An inverse correlation was found between cellular transglutaminase activity and metastatic potential of four cloned cell lines derived from a primary nickel-induced rat rhabdomyosarcoma. Cellular transglutaminase activity as assessed with endogenous cellular protein or exogenous methylated casein was greatest in the clone F9-4/8 which is the least metastasizing. When the putrescine-binding capacity of one cellular derived protein -fibronectin -was examined with exogenous transglutaminase, it was found that the fibronectin derived from the clone F9-4/8 showed the lowest binding capacity compared with those from the other clones. However, when the overall binding capacity of cellular proteins from each cell line was examined no differences could be detected. The results are discussed in the light of the well-known role of fibronectin in cellular adhesion.Transglutaminase Polyamine

Differential expression of isopeptide bonds Nϵ(γ‐glutamyl) lysine in benign and malignant human breast lesions: An immunohistochemical study

“…Similar findings had been reported previously for rat sarcoma by Barnes et al (1985) and Hand et al (1987), who went on to show that the already low TG activity in tumour tissue decreases even further when metastases are present. This temporal relationship between TG and metastasis has been further substantiated by our findings on rat rhabdomyosarcoma cell lines in which TG activity followed the order: least metastasizing > highly metastasizing (Delcros et al, 1986). An inactive form of TG has been detected in rat or hamster fibrosarcoma cells but not in normal tissue (Knight et al, 1990), during the growth of a highly metastatic fibrosarcoma the level of the inactive form increasing at the expense of the active cytosolic form.…”

“…Controls contained thc cryostat scction with all constitucnts cxccpt C a ' ' , which was replaced by 100 mM EDTA. After incubation for I hr at 37"C, with gcntlc shaking, 50 pl incubation mixture were spotted onto a Whatman 3MM filter paper and treated as previously described (Delcros et al, 1986).…”

Differences in the post‐translational modification of proteins by polyamines between weakly and highly metastatic B16 melanoma cells

“…Murine B16-Fl0 and B16-F10Lr6 melanoma cells were obtained from the Division of Cancer Treatment, Tumor Repository NIH (Frederick, MD), and propagated under standard culture conditions (Fidler, 1973). At selected intervals (24,48,72 and 96 hr) cell viability was tested by the exclusion of Trypan blue (0.25%).…”

“…Total cell count was determined periodically by microscopic counting. The origin and properties of the B16-F10 and B16-F10Lr6 melanoma lines have been described (Fidler, 1973). Cultures were found to be free from Mycoplasma species by the Hoechst staining procedure.…”