Transgenic tomato lines containing Ds elements at defined genomic positions as tools for targeted transposon tagging

Knapp, S.; Larondelle, Yvan; Rossberg, Mathias; Furtek, Douglas B.; Theres, Klaus

doi:10.1007/bf00279576

Cited by 57 publications

(19 citation statements)

References 41 publications

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“…Columbia-0 wild-type and las mutant plants were transformed by the floral-dip method (Clough and Bent, 1998). Agrobacterium-mediated transformation of tomato ls leaf explants of cultivar Antimold-B was performed as described by Knapp et al (1994).…”

Section: Transformation Of Arabidopsis Thaliana and Tomatomentioning

confidence: 99%

Specific expression of LATERAL SUPPRESSOR is controlled by an evolutionarily conserved 3′ enhancer

et al. 2011

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SUMMARYAerial plant architecture is largely based on the activity of axillary meristems (AMs), initiated in the axils of leaves. The Arabidopsis gene LATERAL SUPPRESSOR (LAS), which is expressed in well-defined domains at the adaxial boundary of leaf primordia, is a key regulator of AM formation. The precise definition of organ boundaries is an essential step for the formation of new organs in general and for meristem initiation; however, mechanisms leading to these specific patterns are not well understood. To increase understanding of how the highly specific transcript accumulation in organ boundary regions is established, we investigated the LAS promoter. Analysis of deletion constructs revealed that an essential enhancer necessary for complementation is situated about 3.2 kb downstream of the LAS open reading frame. This enhancer is sufficient to confer promoter specificity as upstream sequences in LAS could be replaced by non-specific promoters, such as the 35S minimal promoter. Further promoter swapping experiments using the PISTILLATA or the full 35S promoter demonstrated that the LAS 3¢ enhancer also has suppressor functions, largely overwriting the activity of different 5¢ promoters. Phylogenetic analyses suggest that LAS function and regulation are evolutionarily highly conserved. Homologous elements in downstream regulatory sequences were found in all LAS orthologs, including grasses. Transcomplementation experiments demonstrated the functional conservation of non-coding sequences between Solanum lycopersicum (tomato) and Arabidopsis. In summary, our results show that a highly conserved enhancer/suppressor element is the main regulatory module conferring the boundary-specific expression of LAS.

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Section: Transformation Of Arabidopsis Thaliana and Tomatomentioning

confidence: 99%

Specific expression of LATERAL SUPPRESSOR is controlled by an evolutionarily conserved 3′ enhancer

et al. 2011

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“…So far, contradictory results have been reported on the transposition pattern of the Ac/Ds transposable elements in heterologous systems. Many groups have found that Ac or Ds elements preferentially transpose to linked positions (Jones eta/., 1990;Keller et al, 1993;Knapp et al, 1994), but in other cases clusters of insertion sites were found throughout the genome (Healy et a/., 1993;Knapp eta/., 1994). It is not clear to what extent these results were biased by secondary and subsequent transpositions.…”

Section: Discussionmentioning

confidence: 99%

“…The second approach uses a two-component system consisting of a genetically marked Ds element and a transactivating modified Ac element, which cannot transpose itself (Grevelding et aL, 1992;Healy et aL, 1993;Knapp et al, 1994;Thomas et aL, 1994). The two elements are combined by a genetic cross and after selfing or outcrossing, individuals containing a transposed Ds element but lacking the transposase source can be identified.…”

Section: Introductionmentioning

confidence: 99%

A self‐stabilizing Ac derivative and its potential for transposon tagging

Schmitz¹,

Theres²

1994

The Plant Journal

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SummaryWith the goal of developing a self-stabilizing transposon tagging system, a derivative of the maize transposeble element Ac (De303) harbouring a deletion between bp 246 and 736 has been introduced into tomato (Lycopersicon esculentum) by Agrobacterium tumefaciens-medlated transformation. The deletion removes the major transcription start site, 84 bp of the putative Ac promoter and part of the untranslated leader. Transpositions from the T-DNA, where Ds303 was inserted between the mannopine synthaee 1' promoter and the GUS gene, were observed in four independent transgenic pianta analysed. After transposition the transposed Ds303 elements were stably integrated in the genome and could not transectivate a tester Ds element. However, somatic and germinal transpositions of the transposed Ds303 elements occurred in the presence of a transposeee source.

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“…In fact, the tendency of Ds to transpose to linked sites in Arabidopsis argues that these T-DNA/ox-Ds/oxT-DNA lines are only useful if placed on the Arabidopsis map when one is interested in isolating both mutations and clones corresponding to specific genes. We expect that our 25 T-DNA/ox-Dslox T-DNAs constitute a reasonable collection for the mutagenesis of most of the Arabidopsis genome, as T-DNA insertion appears to be random (Chyi et al, 1986;Knapp et al, 1994;Thomas et al, 1994). In addition, all the chromosomal rearrangements that we may generate using tr-Dslox and T-DNAIox have T-DNA/ox as one breakpoint, and these rearrangements are only useful when mapped.…”

Section: Targeted Dslox Tagging In Arabidopsismentioning

confidence: 99%

“…The locus in this case may be either a mapped gene that had been identified through a corresponding, mutant allele or a mapped, cloned sequence without a genetic identity, such as an Expressed Sequence Tag (EST, Hofte et al, 1993). We propose that a large collection of such mapped T-DNA/ox-Ds/ox T-DNAs should enable the insertional m utagenesis of most of the loci in the Arabidopsis genome, and that such a collection would be a valuable resource for the community (as in Knapp et al, 1994 andThomas eta/., 1994).…”

Section: Introductionmentioning

confidence: 99%

A system for insertional mutagenesis and chromosomal rearrangement using the Ds transposon and Cre—lox

Osborne

Wirtz²,

Baker

1995

The Plant Journal

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SummaryA system for insertional mutagenesis and chromosomal rearrangement in Arabidopsis has been developed. The T-DNA vectors are based on the maize trsnspoeen Ds, Iox sites from the Cre-lox site-specific recombination system, and transcriptional fusions expressing Ac transposese or Cre recombinese. The engineered transposon is termed Dslox. Transposed Dslox insertions were created by crossing plants bearing Dslox with plants expressing Ac transposase, then simultaneously selecting for excision and reinsertion in F 2 seedlings using the herbicides chlorsuifuron and phosphonothricin, respectively. 1=2 plants bearing stable Dslox insertions were identified by scoring for the absence of the Ac transposese T-DNA, using a novel, visual marker in that T-DNA. Two independent Dslox insertions were characterized and placed 5.6 and 16.6 ¢M from their T-DNAIox, which mapped close to m506 on chromosome 4. Plants bearing either of the two different transposed Dsloxs and T-DNAIox were crossed to plants expressing Cre recombinese, which catalyzed recombination between the Iox site in transposed Dslox and the Iox site in TDNAIox. Lox--Iox recombinants were identified selectively amongst progeny of these crosses. Molecular and genetic analysis of the Iox-lox rearrangements indicated that both were inversions. The smaller inversion was germinally transmitted from generation to generation as a simple trait, whereas the larger inversion was not transmitted to progeny of plants bearing the rearrangement.

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Transgenic tomato lines containing Ds elements at defined genomic positions as tools for targeted transposon tagging

Cited by 57 publications

References 41 publications

Specific expression of LATERAL SUPPRESSOR is controlled by an evolutionarily conserved 3′ enhancer

Specific expression of LATERAL SUPPRESSOR is controlled by an evolutionarily conserved 3′ enhancer

A self‐stabilizing Ac derivative and its potential for transposon tagging

A system for insertional mutagenesis and chromosomal rearrangement using the Ds transposon and Cre—lox

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