Transgenic tobacco plants accumulating osmolytes show reduced oxidative damage under freezing stress

Parvanova, D.; Ivanov, Sergey; Konstantinova, T.; Karanov, E.; Atanassov, A.; Tsvetkov, Tsvetan; Alexieva, V.; Djilianov, Dimitar

doi:10.1016/j.plaphy.2003.10.007

Cited by 145 publications

(89 citation statements)

References 42 publications

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“…In tobacco (Nicotiana tabacum), enhanced freezing tolerance has been correlated with the increased ROS scavenging capacity generated by the constitutive expression of a bacterial levansucrase gene producing fructans (Parvanova et al, 2004). The protective role for maltose against oxidative stress was demonstrated by the response of Arabidopsis thaliana to methyl viologen treatment (Scarpeci and Valle, 2008), while in the same species, photosynthetic efficiency was maintained during drought stress by the increased accumulation of soluble sugars (Moustakas et al, 2011).…”

Section: The Direct Participation Of Oligosaccharides In Ros Detoxifimentioning

confidence: 99%

Spatio-Temporal Dynamics of Fructan Metabolism in Developing Barley Grains

et al. 2014

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Barley (Hordeum vulgare) grain development follows a series of defined morphological and physiological stages and depends on the supply of assimilates (mainly sucrose) from the mother plant. Here, spatio-temporal patterns of sugar distributions were investigated by mass spectrometric imaging, targeted metabolite analyses, and transcript profiling of microdissected grain tissues. Distinct spatio-temporal sugar balances were observed, which may relate to differentiation and grain filling processes. Notably, various types of oligofructans showed specific distribution patterns. Levan-and graminan-type oligofructans were synthesized in the cellularized endosperm prior to the commencement of starch biosynthesis, while during the storage phase, inulin-type oligofructans accumulated to a high concentration in and around the nascent endosperm cavity. In the shrunken endosperm mutant seg8, with a decreased sucrose flux toward the endosperm, fructan accumulation was impaired. The tight partitioning of oligofructan biosynthesis hints at distinct functions of the various fructan types in the young endosperm prior to starch accumulation and in the endosperm transfer cells that accomplish the assimilate supply toward the endosperm at the storage phase.

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Section: The Direct Participation Of Oligosaccharides In Ros Detoxifimentioning

confidence: 99%

Spatio-Temporal Dynamics of Fructan Metabolism in Developing Barley Grains

et al. 2014

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“…CAT, POD and SOD are considered major scavenging enzymes involved in removal of ROS produced under various stress conditions, including salinity (Parvanova et al 2004). Among investigated tobacco lines, roots of line 239K treated with 100 mM NaCl exhibited higher CAT activity than line 207B and increased POD activity compared to line 238C and hybrid DH10; those increased activities of both enzymes in line 239K might ensure better scavenging of ROS under 100 mM NaCl.…”

Section: Discussionmentioning

confidence: 98%

“…•-) into oxygen and hydrogen peroxide while POD and CAT are involved in H 2 O 2 detoxifi cation (Bandeoğlu et al 2004, Parvanova et al 2004. Numerous studies emphasize that the antioxidative response strongly correlates with susceptibility and resistance to increased salinity (Acar et al 2001, Tűrkan et al 2005.…”

Section: Introductionmentioning

confidence: 99%

Response of dihaploid tobacco roots to salt stress

Marček¹,

Vidaković-Cifrek²,

Tkalec³

et al. 2017

Acta Botanica Croatica

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-Salinity is a common abiotic factor that affects plant growth and development. Seedlings of tobacco (Nicotiana tabacum L.) F1 hybrid DH10 and three dihaploid lines (207B, 238C and 239K) obtained by diploidization of anther-derived haploids of hybrid DH10 were subjected to 0, 100 and 200 mM NaCl in in vitro conditions for 33 days and the effect on roots was evaluated. In all lines and in the hybrid DH10 exposed to 200 mM NaCl evident root growth inhibition and increased proline content were noticed. However, in some cases lines differed in the activity of antioxidative enzymes, which could account for differences in their salinity tolerance. Increased activity of catalase and peroxidase in roots of line 239K could contribute to the more pronounced salinity tolerance previously reported for shoots of this line.

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“…To achieve tolerance against various abiotic stresses, overexpression of BADH (Moghaieb et al, 2000;Kumar et al, 2004;Yang et al, 2005), CDH (Lilius et al, 1996;Quan et al, 2004) has been carried out in several plant species. Overexpression of coda gene encoding COD has been most widely attempted to develop abiotic stress tolerant transgenic plants (Hayashi et al, 1997;Alia et al, 1998;Huang et al, 2000, Sulpice et al, 2003Parvanova et al, 2004;Prasad and Pardha-Saradhi, 2004). Transgenic rice, tolerant to salt stress has also been developed by the overexpression of codA by various groups (Sakamoto et al, 1998;Mohanty et al, 2002;Su et al, 2006).…”

Section: Targeting Osmotic Homeostasis Machinerymentioning

confidence: 99%

Raising salinity tolerant rice: recent progress and future perspectives

Singh

Ansari

Pareek

et al. 2008

Physiol Mol Biol Plants

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With the rapid growth in population consuming rice as staple food and the deteriorating soil and water quality around the globe, there is an urgent need to understand the response of this important crop towards these environmental abuses. With the ultimate goal to raise rice plant with better suitability towards rapidly changing environmental inputs, intensive efforts are on worldwide employing physiological, biochemical and molecular tools to perform this task. In this regard, efforts of plant breeders need to be duly acknowledged as several salinity tolerant varieties have reached the farmers field. Parallel efforts from molecular biologists have yielded relevant knowledge related to perturbations in gene expression and proteins during stress. Employing transgenic technology, functional validation of various target genes involved in diverse processes such as signaling, transcription, ion homeostasis, antioxidant defense etc for enhanced salinity stress tolerance has been attempted in various model systems and some of them have been extended to crop plant rice too. However, the fact remains that these transgenic plants showing improved performance towards salinity stress are yet to move from 'lab to the land'. Pondering this, we propose that future efforts should be channelized more towards multigene engineering that may enable the taming of this multigene controlled trait. Recent technological achievements such as the whole genome sequencing of rice is leading to a shift from single gene based studies to genome wide analysis that may prove to be a boon in re-defining salt stress responsive targets.

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Transgenic tobacco plants accumulating osmolytes show reduced oxidative damage under freezing stress

Cited by 145 publications

References 42 publications

Spatio-Temporal Dynamics of Fructan Metabolism in Developing Barley Grains

Spatio-Temporal Dynamics of Fructan Metabolism in Developing Barley Grains

Response of dihaploid tobacco roots to salt stress

Raising salinity tolerant rice: recent progress and future perspectives

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