Transgenic Tea Over-expressing Solanum tuberosum Endo-1,3-beta-d-glucanase Gene Conferred Resistance Against Blister Blight Disease

Singh, Harvinder; Hazarika, Pranita; Agarwala, Niraj; Bhattacharyya, Neelakshi; Bhagawati, Prasenjit; Gohain, Bornali; Bandyopadhyay, Tirthankar; Bharalee, Raju; Gupta, Sushmita; Deka, Manab; Das, Sudripta

doi:10.1007/s11105-017-1063-x

Cited by 19 publications

(6 citation statements)

References 39 publications

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“…Chen et al [ 54 ] used rice to study grazing-induced pathway, and found the expression level of 1,3-beta-glucosidase increased. This gene was reported to be associated with resistance in plant, cell wall construction and modification [ 55 , 56 ]. In our study, gene related to beta-glucosidase was upregulated under grazing, conferring resistance on S .…”

Section: Discussionmentioning

confidence: 99%

De novo assembly, annotation, marker discovery, and genetic diversity of the Stipa breviflora Griseb. (Poaceae) response to grazing

et al. 2020

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Grassland is one of the most widely-distributed ecosystems on Earth and provides a variety of ecosystem services. Grasslands, however, currently suffer from severe degradation induced by human activities, overgrazing pressure and climate change. In the present study, we explored the transcriptome response of Stipa breviflora, a dominant species in the desert steppe, to grazing through transcriptome sequencing, the development of simple sequence repeat (SSR) markers, and analysis of genetic diversity. De novo assembly produced 111,018 unigenes, of which 88,164 (79.41%) unigenes were annotated. A total of 686 unigenes showed significantly different expression under grazing, including 304 and 382 that were upregulated and downregulated, respectively. These differentially expressed genes (DEGs) were significantly enriched in the “alpha-linolenic acid metabolism” and “plant-pathogen interaction” pathways. Based on transcriptome sequencing data, we developed eight SSR molecular markers and investigated the genetic diversity of S. breviflora in grazed and ungrazed sites. We found that a relatively high level of S. breviflora genetic diversity occurred under grazing. The findings of genes that improve resistance to grazing are helpful for the restoration, conservation, and management of desert steppe.

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Section: Discussionmentioning

confidence: 99%

De novo assembly, annotation, marker discovery, and genetic diversity of the Stipa breviflora Griseb. (Poaceae) response to grazing

et al. 2020

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“…Previous studies revealed that the defense-related response played an essential role in improving the disease resistance of plants. GLU enhances the tolerant of rough lemon rootstock against foot rot (Sandhu et al, 2019), improves the inhibition of rice against Rhizoctonia solani (Kumar et al, 2018), reduces the rice sheath blight (Kaur et al, 2021), and confers tea tree resistance against blister blight disease (Singh et al, 2018). POD is involved in the resistance response of coffee varieties to Colletotrichum kahawae (Diniz et al, 2019), potato to P. infestans , Arabidopsis thaliana to Botrytis cinerea, Colletotrichum higginsianum, and Pectobacterium carotovorum (Zhao et al, 2019), Citrus sinensis to citrus bacterial canker (Li et al, 2020).…”

Section: Discussionmentioning

confidence: 99%

Efficacy of Dimethyl Trisulfide on the Suppression of Ring Rot Disease Caused by Botryosphaeria dothidea and Induction of Defense-Related Genes on Apple Fruits

et al. 2022

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Apple ring rot caused by Botryosphaeria dothidea is prevalent in main apple-producing areas in China, bringing substantial economic losses to the growers. In the present study, we demonstrated the inhibitory effect of dimethyl trisulfide (DT), one of the main activity components identified in Chinese leek (Allium tuberosum) volatile, on the apple ring rot on postharvest fruits. In in vitro experiment, 250 μL/L DT completely suppressed the mycelia growth of B. dothidea. In in vivo experiment, 15.63 μL/L DT showed 97% inhibition against the apple ring rot on postharvest fruit. In addition, the soluble sugar content, vitamin C content, and the soluble sugar/titratable acidity ratio of the DT-treated fruit were significantly higher than those of the control fruit. On this basis, we further explored the preliminary underlying mechanism. Microscopic observation revealed that DT seriously disrupted the normal morphology of B. dothidea. qRT-PCR determination showed the defense-related genes in DT-treated fruit were higher than those in the control fruit by 4.13–296.50 times, which showed that DT inhibited apple ring rot on postharvest fruit by suppressing the growth of B. dothidea, and inducing the defense-related genes in apple fruit. The findings of this study provided an efficient, safe, and environment-friendly alternative to control the apple ring rot on apple fruit.

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“…Transgenic Arabidopsis plants expressing grapevine b-1,3-glucanase (VvGHF17) confers resistance to Colletotrichum higginsianum and Botrytis cinerea [73]. Furthermore, tea with transgenic overexpression of the endo-1,3-D-glucanase gene, which expresses a potato glucanase, significantly improved tolerance to the blister blight fungus Exobasidium vexans [74]. Recently, oil palm resistance to G. boninense was improved by transgenic overexpression of M. sativa glucanase (AGLU1) [75].…”

Section: Transgenic Plants Expressing Antifungal Activitiesmentioning

confidence: 99%

Pathogenesis-Related Proteins and Their Transgenic Expression for Developing Disease-Resistant Crops: Strategies Progress and Challenges

Kaur¹,

Kaur²,

Kaur³

et al. 2023

Case Studies of Breeding Strategies in Major Plant Species

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Various pathogenic microorganisms (such as fungi, bacteria, viruses and nematodes) affect plant viability and productivity. However, plants combat these pathogens by inducing their defense mechanism to sustain their fitness. The aggregation of pathogenesis-related (PR) proteins in response to invading pathogens is a crucial component of a plant’s self-defense mechanism. PR proteins induce innate resistance in plants through fungal cell wall disintegration, membrane permeabilization, transcriptional suppression, and ribosome inactivation. Earlier studies have demonstrated their crucial role in determining resistance against phytopathogens, making them a promising candidate for developing disease-resistant crop varieties. Plant genetic engineering is a potential approach for developing disease-resistant transgenic crops by employing several PR genes (thaumatin, osmotin-like proteins, chitinases, glucanases, defensins, thionins, oxalate oxidase, oxalate oxidases like proteins/germin-like proteins and LTPs). Furthermore, the overexpression of PR proteins enhances the resistance against phytopathogens. As a result, this chapter gives an overview of PR proteins, including their classification, functional characterization, signaling pathways, mode of action and role in defense against various phytopathogens. It also highlights genetic engineering advances in utilizing these genes singly or synergistically against various phytopathogens to impart disease resistance. Various challenges faced with the products of transgenic technology and synergistic expression of different groups of PR proteins were also discussed.

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Transgenic Tea Over-expressing Solanum tuberosum Endo-1,3-beta-d-glucanase Gene Conferred Resistance Against Blister Blight Disease

Cited by 19 publications

References 39 publications

De novo assembly, annotation, marker discovery, and genetic diversity of the Stipa breviflora Griseb. (Poaceae) response to grazing

De novo assembly, annotation, marker discovery, and genetic diversity of the Stipa breviflora Griseb. (Poaceae) response to grazing

Efficacy of Dimethyl Trisulfide on the Suppression of Ring Rot Disease Caused by Botryosphaeria dothidea and Induction of Defense-Related Genes on Apple Fruits

Pathogenesis-Related Proteins and Their Transgenic Expression for Developing Disease-Resistant Crops: Strategies Progress and Challenges

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