Transgenic Tall Fescue (Festuca arundinacea) and Red Fescue (F. rubra) Plants from Microprojectile Bombardment of Embryogenic Suspension Cells

Spangenberg, Germán; Wang, Z.Y.; Wu, Xiaolin; Nagel, J.; Iglesias, Victor A.; Potrykus, Ingo

doi:10.1016/s0176-1617(11)81283-6

Cited by 77 publications

(30 citation statements)

References 28 publications

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“…Because biolistic transformation is a physical process that involves only one biological system, it is a fairly reproducible procedure that can be easily adapted from one laboratory to another laboratory. Transgenic forage plants have been obtained by microprojectile bombardment of embryogenic cells in tall fescue (Spangenberg et al, 1995a;Cho et al, 2000;Wang et al, 2001bWang et al, , 2003aChen et al, 2003Chen et al, , 2004, perennial ryegrass (Spangenberg et al, 1995b;Dalton et al, 1999;Altpeter et al, 2000;Xu et al, 2001;Petrovska et al, 2004;Chen et al, 2005), Italian ryegrass (Ye et al, 1997(Ye et al, , 2001Dalton et al, 1999;Petrovska et al, 2004), orchardgrass (Denchev et al, 1997;Cho et al, 2001), Kentucky bluegrass (Ha et al, 2001;Gao et al, 2006), and Russian wildrye (Wang et al, 2004a) (Table 1).…”

Section: Methods Employed For Transformationmentioning

confidence: 99%

Cool‐Season Forage Grasses

Wang

Yamada

2008

Compendium of Transgenic Crop Plants

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Cool‐season grasses provide most of the forage consumed by ruminant animals in temperate areas of the world. Transgenic plants have been obtained for a number of cool‐season grass species, including tall fescue, perennial ryegrass, Italian ryegrass, orchardgrass, Kentucky bluegrass, and Russian wildrye. This chapter describes the origin, taxonomy, cytological features, economic importance, and biotechnological improvement of these species. Progresses in genetic transformation techniques and the use of transgenic technology for improvement of these species are summarized.

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Section: Methods Employed For Transformationmentioning

confidence: 99%

Cool‐Season Forage Grasses

Wang

Yamada

2008

Compendium of Transgenic Crop Plants

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“…The pAcH1 vector was previously constructed and has been used successfully in plant transformation experiments [30][31][32][33][34]. The GUS marker gene was also cloned as a positive control.…”

Section: Production Of Transgenic Ryegrass Plantsmentioning

confidence: 99%

“…Following agarose gel electrophoresis, the resulting DNA fragment was purified from the agarose gel prior to being used for plant transformation to produce DNA without vector backbone sequences. The pAcH1 vector previously constructed and used successfully in plant transformation experiments was also digested with restriction enzymes to produce a DNA fragment for the expression of the selectable marker only [30][31][32][33][34].…”

Section: Production Of Transgenic Ryegrass Plantsmentioning

confidence: 99%

Re-Programming Photosynthetic Cells of Perennial Ryegrass (Lolium perenne L) for Fructan Biosynthesis through Transgenic Expression of Fructan Biosynthetic Genes under the Control of Photosynthetic Promoters

et al. 2017

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High molecular weight fructans are the main class of water-soluble carbohydrate used for energy storage in many temperate grass species including perennial ryegrass (Lolium perenne L.). As well as being important readily mobilisable energy reserves for the plant, fructans are also involved in stress tolerance. Fructans are also readily digested by grazing ruminants and hence are a valuable source of energy for sheep, beef and dairy production systems in temperate regions. This paper describes the re-programming of the expression of fructan biosynthesis genes through the transgenic manipulation of 6-glucose fructosyltransferase (6G-FFT) and sucrose:sucrose 1-fructosyl-transferase (1-SST) in perennial ryegrass. Transgenic events were developed with altered fructan accumulation patterns with increases in fructan accumulation and greatly increased accumulation of fructan in leaf blades as opposed to the traditional site of fructan accumulation in the pseudostem. This altered site of fructan accumulation has potential benefits for animal production as leaf blades form the major part of the diet of grazing ruminants. Some of the transgenic events also exhibited enhanced biomass production. This combination of high quality and enhanced yield is of great interest to forage plant breeders and whilst the expression of these phenotypes needs to be confirmed under field conditions, the identification and characterisation of the transgenic events described in this paper validate the potential for the manipulation of fructan biosynthesis in perennial ryegrass.

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“…Paromomycin selection has been established in various turf and forage grasses such as perennial ryegrass [42], red fescue [59], bahiagrass [60] and cereals (rye; [61,62]). The hph gene encoding hygromycin phosphotransferase, allows effective selection and is used at a range of concentrations from 20 mg/l in orchardgrass [63] to 250 mg/l in transformed tall fescue [57,[64][65][66]. Phosphomannose isomerase (PMI), an enzyme not present in plants, catalyses the reversible interconversion of mannose 6-phosphate and fructose 6-phosphate.…”

Section: Selection and Plant Regenerationmentioning

confidence: 99%