Transgenic Tall Fescue and Maize with Resistance to ALS-Inhibiting Herbicides

Sato, Hiroko; Takamizo, Tadashi; Horita, Junko; Kawai, Kiyoshi; Kaku, Koichiro; Shimizu, Tsutomu

doi:10.5772/13099

Cited by 1 publication

(1 citation statement)

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“… Frame et al (2011) described modification of culture media to elevate the transformation frequency of inbred B104. Sato et al (2011) reported the selection of transformants of Japanese inbred line Mi29 by the combination of mutant acetolactate synthase (ALS) and a herbicide, Bispyribac-sodium; transformants were obtained from 30% of the immature embryos infected. Akoyi et al (2013) adjusted plant growth regulators in culture media for immature embryos from five tropical maize varieties.…”

Section: Progress In Maize Transformationmentioning

confidence: 99%

Progress of cereal transformation technology mediated by Agrobacterium tumefaciens

2014

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Monocotyledonous plants were believed to be not transformable by the soil bacterium Agrobacterium tumefaciens until two decades ago, although convenient protocols for infection of leaf disks and subsequent regeneration of transgenic plants had been well established in a number of dicotyledonous species by then. This belief was reinforced by the fact that monocotyledons are mostly outside the host range of crown gall disease caused by the bacterium and by the failures in trials in monocotyledons to mimic the transformation protocols for dicotyledons. However, a key reason for the failure could have been the lack of active cell divisions at the wound sites in monocotyledons. The complexity and narrow optimal windows of critical factors, such as genotypes of plants, conditions of the plants from which explants are prepared, tissue culture methods and culture media, pre-treatments of explants, strains of A. tumefaciens, inducers of virulence genes, transformation vectors, selection marker genes and selective agents, kept technical hurdles high. Eventually it was demonstrated that rice and maize could be transformed by co-cultivating cells of callus cultures or immature embryos, which are actively dividing or about to divide, with A. tumefaciens. Subsequently, these initial difficulties were resolved one by one by many research groups, and the major cereals are now transformed quite efficiently. As many as 15 independent transgenic events may be regenerated from a single piece of immature embryo of rice. Maize transformation protocols are well established, and almost all transgenic events deregulated for commercialization after 2003 were generated by Agrobacterium-mediated transformation. Wheat, barley, and sorghum are also among those plants that can be efficiently transformed by A. tumefaciens.

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Section: Progress In Maize Transformationmentioning

confidence: 99%