Transgenic switchgrass (<i>Panicum virgatum</i> L.) biomass is increased by overexpression of switchgrass sucrose synthase (<i>PvSUS1</i>)

Poovaiah, Charleson R.; Mazarei, Mitra; Decker, Stephen R.; Turner, Geoffrey B.; Sykes, Robert; Davis, Mark F.; Stewart, C. Neal

doi:10.1002/biot.201400499

Cited by 46 publications

(32 citation statements)

References 50 publications

(69 reference statements)

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“…Poovaiah et al . (Poovaiah et al ., ) overexpressed a SuSy transgene in the biofuel feedstock switchgrass ( Panicum virgatum ), achieving increased height and biomass in some transformants. In Populus alba x grandidentata (hybrid poplar), overexpression of SuSy caused increases in soluble carbohydrates and cellulose, and a decrease in cell wall‐derived arabinose (Coleman et al ., ).…”

Section: Discussionmentioning

confidence: 99%

Predicting future biomass yield in Miscanthus using the carbohydrate metabolic profile as a biomarker

et al. 2017

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In perennial energy crop breeding programmes, it can take several years before a mature yield is reached when potential new varieties can be scored. Modern plant breeding technologies have focussed on molecular markers, but for many crop species, this technology is unavailable. Therefore, prematurity predictors of harvestable yield would accelerate the release of new varieties. Metabolic biomarkers are routinely used in medicine, but they have been largely overlooked as predictive tools in plant science. We aimed to identify biomarkers of productivity in the bioenergy crop, Miscanthus, that could be used prognostically to predict future yields. This study identified a metabolic profile reflecting productivity in Miscanthus by correlating the summer carbohydrate composition of multiple genotypes with final yield 6 months later. Consistent and strong, significant correlations were observed between carbohydrate metrics and biomass traits at two separate field sites over 2 years. Machine‐learning feature selection was used to optimize carbohydrate metrics for support vector regression models, which were able to predict interyear biomass traits with a correlation (R) of >0.67 between predicted and actual values. To identify a causal basis for the relationships between the glycome profile and biomass, a 13C‐labelling experiment compared carbohydrate partitioning between high‐ and low‐yielding genotypes. A lower yielding and slower growing genotype partitioned a greater percentage of the 13C pulse into starch compared to a faster growing genotype where a greater percentage was located in the structural biomass. These results supported a link between plant performance and carbon flow through two rival pathways (starch vs. sucrose), with higher yielding plants exhibiting greater partitioning into structural biomass, via sucrose metabolism, rather than starch. Our results demonstrate that the plant metabolome can be used prognostically to anticipate future yields and this is a method that could be used to accelerate selection in perennial energy crop breeding programmes.

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Section: Discussionmentioning

confidence: 99%

Predicting future biomass yield in Miscanthus using the carbohydrate metabolic profile as a biomarker

et al. 2017

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“…This is most likely due to the reduction in photosynthate partitioning towards generative development, relative to wild-type plants, under floral induction conditions. Enhanced biomass production as a result of the flowerless trait could be useful in various energy crops (Kalluri et al, 2014;Poovaiah et al, 2015;Torney et al, 2007).…”

Section: Discussionmentioning

confidence: 99%

AnAGAMOUSintron‐driven cytotoxin leads to flowerless tobacco and produces no detrimental effects on vegetative growth of either tobacco or poplar

Wei

Fang

et al. 2016

Plant Biotechnology Journal

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SummaryFlowerless trait is highly desirable for poplar because it can prevent pollen‐ and seed‐mediated transgene flow. We have isolated the second intron of PTAG2, an AGAMOUS (AG) orthologue from Populus trichocarpa. By fusing this intron sequence to a minimal 35S promoter sequence, we created two artificial promoters, fPTAG2I (forward orientation of the PTAG2 intron sequence) and rPTAG2I (reverse orientation of the PTAG2 intron sequence). In tobacco, expression of the β‐glucuronidase gene (uidA) demonstrates that the fPTAG2I promoter is non‐floral‐specific, while the rPTAG2I promoter is active in floral buds but with no detectable vegetative activity. Under glasshouse conditions, transgenic tobacco plants expressing the Diphtheria toxin A (DT‐A) gene driven by the rPTAG2I promoter produced three floral ablation phenotypes: flowerless, neuter (stamenless and carpel‐less) and carpel‐less. Further, the vegetative growth of these transgenic lines was similar to that of the wild‐type plants. In field trials during 2014 and 2015, the flowerless transgenic tobacco stably maintained its flowerless phenotype, and also produced more shoot and root biomass when compared to wild‐type plants. In poplar, the rPTAG2I::GUS gene exhibited no detectable activity in vegetative organs. Under field conditions over two growing seasons (2014 to the end of 2015), vegetative growth of the rPTAG2I::DT‐A transgenic poplar plants was similar to that of the wild‐type plants. Our results demonstrate that the rPTAG2I artificial promoter has no detectable activities in vegetative tissues and organs, and the rPTAG2I::DT‐A gene may be useful for producing flowerless poplar that retains normal vegetative growth.

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“…Taking together, the function of SuS is for the sucrose synthesis only in August, however for sucrose decomposition for other months. Poovaiah et al (2015) increased the biomass of switchgrass through overexpression of switchgrass sucrose synthase gene PvSUS1. This suggested that cassava starch might be increased through similar approach.…”

Section: The Time Course Of Cassava Leaf Sus Synthesis Direction and mentioning

confidence: 99%

Time Course of Starch Biosynthesis Enzymes Activity and Root Tuber Starch of Four Cassava Cultivars

Huang¹,

Luo²,

Huang³

et al. 2017

JAS

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To understand the accumulation rule of cassava root tuber starch, the amylose, amylopectin and total starch content of fresh root tuber, the enzyme activities of sucrose synthase (SuS, EC 2.4.1.13) and sucrose phosphate synthase (SPS, EC 2.4.1.14) of leaves, the enzyme activities of ADP-glucose pyrophosphorylase (AGPase, EC 2.7.7.27), soluble starch synthase (SSS, EC 2.4.1.21) and starch branching enzyme (SBE, EC. 2.4.1.18) of tubers were assessed by using cultivars SC201, SC205, GR891 and GR911 leaves and root tubers during their growth period, respectively. The results as follows: the enzyme activity of leaf SPS and the synthesis direction of SuS showed the highest at August, 2010, however the enzyme activity of the decomposing direction of SuS formed parabolic curve; the enzyme activity of tuber AGPase showed an increased then decreased single peak curve, the enzyme activity of tuber SSS oscillating decreased, while the enzyme activity of SBE was relatively stable; the amylose, amylopectin and total starch contents of fresh cassava tuber were all gradually increased along with the growth period. This research would enrich our knowledge of the time course of amylose, amylopectin and total starch contents of fresh cassava tuber, and above related enzyme activities.

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Transgenic switchgrass (Panicum virgatum L.) biomass is increased by overexpression of switchgrass sucrose synthase (PvSUS1)

Cited by 46 publications

References 50 publications

Predicting future biomass yield in Miscanthus using the carbohydrate metabolic profile as a biomarker

Predicting future biomass yield in Miscanthus using the carbohydrate metabolic profile as a biomarker

AnAGAMOUSintron‐driven cytotoxin leads to flowerless tobacco and produces no detrimental effects on vegetative growth of either tobacco or poplar

Time Course of Starch Biosynthesis Enzymes Activity and Root Tuber Starch of Four Cassava Cultivars

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