Transgenic Sweet Orange expressing hairpin CP-mRNA in the interstock confers tolerance to citrus psorosis virus in the non-transgenic scion

Francesco, Agustina De; Simeone, Melina; Gómez, Cristina; Costa, N. B.; García, María Laura

doi:10.1007/s11248-020-00191-1

Cited by 13 publications

(12 citation statements)

References 29 publications

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“…It is established, using a triple plant system generated by grafting, that ihpCP-transcripts translocate through the graft, conferring silencing induction in the scion by the expression of ihpCP in the interstock and the protection to the non-transgenic scion against CPsV. These results corroborate the idea that grafting is a promising biotechnological alternative to protect woody plants against viral infection in vegetatively propagated material [ 102 ].…”

Section: Strategies To Control the Diseasesupporting

confidence: 57%

“…This means that resistance could be transmitted bi-directionally in a triple plant system generated by grafting. This approach is therefore considered as a promising biotechnological alternative to protect woody plants against viral infections in vegetatively propagated plants [ 102 ]. Currently, genome editing ensured by clustered regularly interspaced short palindromic repeats (CRISPR)-based systems is considered as a crucial genetic manipulation tool that can be applied to numerous crops, including citrus.…”

Section: Discussionmentioning

confidence: 99%

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Citrus Psorosis Virus: Current Insights on a Still Poorly Understood Ophiovirus

et al. 2020

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Citrus psorosis was reported for the first time in Florida in 1896 and was confirmed as a graft-transmissible disease in 1934. Citrus psorosis virus (CPsV) is the presumed causal agent of this disease. It is considered as a type species of the genus Ophiovirus, within the family Aspiviridae. CPsV genome is a negative single-stranded RNA (-ssRNA) with three segments. It has a coat protein (CP) of 48 kDa and its particles are non-enveloped with naked filamentous nucleocapsids existing as either circular open structures or collapsed pseudo-linear forms. Numerous rapid and sensitive immuno-enzymatic and molecular-based detection methods specific to CPsV are available. CPsV occurrence in key citrus growing regions across the world has been spurred the establishment of the earliest eradication and virus-free budwood programs. Despite these efforts, CPsV remains a common and serious challenge in several countries and causes a range of symptoms depending on the isolate, the cultivar, and the environment. CPsV can be transmitted mechanically to some herbaceous hosts and back to citrus. Although CPsV was confirmed to be seedborne, the seed transmission is not efficient. CPsV natural spread has been increasing based on both CPsV surveys detection and specific CPsV symptoms monitoring. However, trials to ensure its transmission by a soil-inhabiting fungus and one aphid species have been unsuccessful. Psorosis disease control is achieved using CPsV-free buds for new plantations, launching budwood certification and indexing programs, and establishing a quarantine system for the introduction of new varieties. The use of natural resistance to control CPsV is very challenging. Transgenic resistance to at least some CPsV isolates is now possible in at least some sweet orange varieties and constitutes a promising biotechnological alternative to control CPsV. This paper provides an overview of the most remarkable achievements in CPsV research that could improve the understanding of the disease and lead the development of better control strategies.

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Section: Strategies To Control the Diseasesupporting

confidence: 57%

Section: Discussionmentioning

confidence: 99%

Citrus Psorosis Virus: Current Insights on a Still Poorly Understood Ophiovirus

et al. 2020

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“…rootstock or interstock) has the potential to spread into the grafted parts of the plant so as to confer resistance to disease to the whole plant, including fruit. This results in fruits that are not genetically modified (GM), but protected by the presence of target-specific degradable small RNA molecules (De Francesco et al 2020;Limera et al 2017;Zhao and Song 2014).…”

Section: The Importance Of Rnai In Sustainable Agriculturementioning

confidence: 99%

RNAi: What is its position in agriculture?

et al. 2020

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RNA interference (RNAi) is being developed and exploited to improve plants by modifying endogenous gene expression as well as to target pest and pathogen genes both within plants (i.e. host-induced gene silencing) and/or as topical applications (e.g. spray-induced gene silencing). RNAi is a natural mechanism which can be exploited to make a major contribution towards integrated pest management and sustainable agricultural strategies needed worldwide to secure current and future food production. RNAi plants are being assessed and regulated using existing regulatory frameworks for GMO. However, there is an urgent need to develop appropriate science-based risk assessment procedures for topical RNAi applications within existing plant protection products legislation. Keywords RNAi • dsRNA • Biosafety • Agriculture • Regulations • HIGS • SIGS Key message • RNAi is a natural mechanism found in most eukaryotic organisms in nature and can be exploited to improve plant health. • RNAi-based technology is already being exploited, and the realized examples confirm its great potential in a range of areas of crop production and protection.

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“…In addition, we further analysed whether symptom onset occurs only above a threshold viral load. For this purpose, we compared the symptom score (SS) on a scale ranging from 0 to 9, as considered by De Francesco et al (2020), and the viral load estimated by RT‐qPCR as the number of CP gene fragments per 100 Ubiquitin gene fragments, used as an endogenous reference, in all 29 positive samples (Figure 2). Although five samples with less than 100 CP fragments were found with no symptoms (SS = 0), no significant association was observed between viral load and SS, probably due to the variable distribution of viral RNA in the small piece of leaf tissue taken for the analysis.…”

Section: Discussionmentioning

confidence: 99%

Detection of citrus psorosis virus by RT‐qPCR validated by diagnostic parameters

et al. 2021

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Citrus psorosis virus (CPsV) is the causal agent of psorosis, an important disease of citrus. Sanitary and certification programmes helped reduce disease damage caused by psorosis and other graft‐transmissible diseases in many citrus‐growing regions. For quarantine and certification programmes, most of these diseases are currently diagnosed using biological indexing (BI) on sensitive indicator plants. In the case of citrus psorosis, CPsV can be detected by molecular methods such as quantitative reverse transcription PCR (RT‐qPCR), which is cheaper and faster than BI, but sensitivity, reliability, and reproducibility of both procedures have not been compared so far. In this work, 128 plants from Argentina and Uruguay were analysed using BI and CPsV detection by the RT‐qPCR assay. Almost perfect agreement between both diagnostic procedures and sensitivity, specificity, and estimated likelihood ratios indicate that RT‐qPCR is equivalent to BI for citrus psorosis diagnosis, thus providing confidence in the quick diagnostic procedure to monitor the sanitary status of citrus trees.

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Transgenic Sweet Orange expressing hairpin CP-mRNA in the interstock confers tolerance to citrus psorosis virus in the non-transgenic scion

Cited by 13 publications

References 29 publications

Citrus Psorosis Virus: Current Insights on a Still Poorly Understood Ophiovirus

Citrus Psorosis Virus: Current Insights on a Still Poorly Understood Ophiovirus

RNAi: What is its position in agriculture?

Detection of citrus psorosis virus by RT‐qPCR validated by diagnostic parameters

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