Transgenic Rootstock Protein Transmission in Grapevines

Li, Z. T.; Kelley, Karen; Dhekney, Sadanand A.; Aman, M. Van; Tattersall, J.; Gray, D. J.

doi:10.17660/actahortic.2007.738.99

Cited by 23 publications

(15 citation statements)

References 8 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The results reported so far have been inconsistent in this regard. Dutt et al (2007) and Bortolotti et al (2005) have reported that transgene proteins were found in sap and phloem of non-transgenic scions grafted onto transgenic rootstock in grape and tobacco, respectively, but Youk et al (2009) reported that no transgenic products were translocated from transgenic rootstock to non-transgenic scion in watermelon.…”

Section: Introductionmentioning

confidence: 99%

Effects of transgenic rootstocks on growth and development of non-transgenic scion cultivars in apple

Smolka

Heikelt

et al. 2010

Transgenic Res

View full text Add to dashboard Cite

Although cultivation of genetic modified (GM) annual crops has been steadily increasing in the recent 10 years, the commercial cultivation of GM fruit tree is still very limited and reports of field trials on GM fruit trees are rare. This is probably because development and evaluation of GM fruit trees require a long period of time due to long life cycles of trees. In this study, we report results from a field trial on three rolB transgenic dwarfing apple rootstocks of M26 and M9 together with non-transgenic controls grafted with five non-transgenic scion cultivars. We intended to investigate the effects of transgenic rootstock on non-transgenic scion cultivars under natural conditions as well as to evaluate the potential value of using the rolB gene to modify difficult-to-root rootstocks of fruit trees. The results showed that all rolB transgenic rootstocks significantly reduced vegetative growth including tree height regardless of scion cultivar, compared with the non-transgenic rootstocks. Flowering and fruiting were also decreased for cultivars grown on the transgenic rootstocks in most cases, but the fruit quality was not clearly affected by the transgenic rootstocks. Cutting experiment and RT-PCR analysis showed that the rolB gene was stably expressed under field conditions. PCR and RT-PCR analyses displayed that the rolB gene or its mRNA were not detectable in the scion cultivars, indicating no translocation of the transgene or its mRNA from rootstock to scion. Our results suggest that rolB modified rootstocks should be used in combination with vigorous scion cultivars in order to obtain sufficient vegetative growth and good yield. Alternatively, the rolB gene could be used to dwarf vigorous rootstocks of fruit trees or produce bonzai plants as it can significantly reduce the vegetative growth of plants.

show abstract

Section: Introductionmentioning

confidence: 99%

Effects of transgenic rootstocks on growth and development of non-transgenic scion cultivars in apple

Smolka

Heikelt

et al. 2010

Transgenic Res

View full text Add to dashboard Cite

show abstract

“…To test this hypothesis, transgenic Thompson Seedless grape expressing the Shiva-1 lytic peptide gene was treated as a rootstock. Nontransgenic Cabernet Sauvignon and Thompson Seedless grape scions were grafted onto this rootstock (Dutt et al 2007 ). The presence of Shiva-1 peptide in xylem sap of the scion was confi rmed by ELISA and demonstrates the potential to protect fruit from Pierce ' s disease which is caused by the xylem-limited bacteria, Xylella fastidiosa (Dutt et al 2007 ).…”

Section: Other New Breeding Techniquesmentioning

confidence: 99%

“…Nontransgenic Cabernet Sauvignon and Thompson Seedless grape scions were grafted onto this rootstock (Dutt et al 2007 ). The presence of Shiva-1 peptide in xylem sap of the scion was confi rmed by ELISA and demonstrates the potential to protect fruit from Pierce ' s disease which is caused by the xylem-limited bacteria, Xylella fastidiosa (Dutt et al 2007 ). The edible parts of the plant are non-GM but they are exposed to a recombinant protein produced by GM roots.…”

Section: Other New Breeding Techniquesmentioning

confidence: 99%

Challenging regulations: Managing risks in crop biotechnology

Jones¹

2015

Food and Energy Security

View full text Add to dashboard Cite

show abstract

“…Examples include the Cry1Ab protein (about 100 kDa) for insect resistance and the bar protein (about 22 kDa) for herbicide resistance) where there is no documentation of long-distance transport of these proteins. Consequently, little has been achieved in using transported transgene proteins to improve grafted woody plants, although the potential has been reported in grapevine (Vitis vinifera), where transgenic peptides/proteins produced in rootstocks for disease resistance were present in nontransgenic scions (Agüero et al, 2005;Dutt et al, 2007). However, rootstock-to-scion movement of transgenic proteins was not detected in transgenic plum containing the Gastrodia antifungal protein (GAFP-1) (Nagel et al, 2010) or from the 'M26' apple rootstock containing the root-promoting rolB gene (Smolka et al, 2010), indicating that engineering rootstocks with low mobility transgenic proteins may result in less concern about transgenic products in nontransgenic scions.…”

Section: Mobility Of Ge Proteins/peptidesmentioning

confidence: 99%

Grafting of Genetically Engineered Plants

Song

Walworth

Loescher

2015

J. Amer. Soc. Hort. Sci.

View full text Add to dashboard Cite

Grafting is a well-established agricultural practice, and it now has implications for the commercialization of transgenic plants. In transgrafted plants, only one part (scion or rootstock) is transgenic with the other part untransformed. However, transgenes may affect both mobile and immobile endogenous metabolites (e.g., RNAs, proteins, and phytohormones) and mobility has implications for transgrafting. In the phloem, long-distance transport of mobile metabolites can play important roles in plant development and signaling. In a transgrafted plant, an immobile transgene product (ITP) is not likely to be translocated across the graft union. In contrast, mobile transgene products (MTP) may be translocated across the graft. Regardless of the mobility of transgene products (TP), interaction of transgenic and nontransgenic parts in transgrafted plants through either the MTP or ITP has been demonstrated to be effective in facilitating changes in nontransgenic portions of the plant. Consequently, and particularly in fruit crops, transgrafting provides the potential for improving products from their nontransgenic parts with the possibility of minimizing the controversy over transgenic crops. This review focuses mainly on the mobility of TP and effects on the whole transgrafted plant.

show abstract

Transgenic Rootstock Protein Transmission in Grapevines

Cited by 23 publications

References 8 publications

Effects of transgenic rootstocks on growth and development of non-transgenic scion cultivars in apple

Effects of transgenic rootstocks on growth and development of non-transgenic scion cultivars in apple

Challenging regulations: Managing risks in crop biotechnology

Grafting of Genetically Engineered Plants

Contact Info

Product

Resources

About