Transgenic rice plants that overexpress transcription factors RF2a and RF2b are tolerant to rice tungro virus replication and disease

Dai, Shaotao; Wei, Xiaoping; Alfonso, Antonio A.; Pei, Liping; Duque, U.G.; Zhang, Zhihong; Babb, Gina M.; Beachy, Roger N.

doi:10.1073/pnas.0810303105

Cited by 62 publications

(42 citation statements)

References 20 publications

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“…Expression of the RF2a and RF2b genes provided RTBV resistance (Dai et al 2008). There are also several cases of improvement of RYMV resistance by a transgenic approach (Pinto et al 1999;Kouassi et al 2006).…”

Section: Conclusion and Recommendations For Future Transgenic Approamentioning

confidence: 99%

Potential Candidate Genes for Improving Rice Disease Resistance

Delteil

Zhang²,

Lessard³

et al. 2010

Rice

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Diseases caused by fungal and bacterial pathogens like Magnaporthe oryzae and Xanthomonas oryzae pv. oryzae are responsible for considerable yield loss. Up to now, in rice, the modification of the expression of more than 60 genes from diverse origins has shown beneficial effects with respect to disease resistance. In this paper, we review this large set of data to identify the best genes and strategies to achieve disease resistance by transgenic approaches. Altered expression of genes involved in signal transduction and transcription may lead to many unwanted side effects, like lesion mimic phenotypes. Moreover, modification of resistance to abiotic stress has been neglected and should be carefully examined in the future. Genes like resistance genes and pathogenesis-related genes can confer broad spectrum and high levels of resistance to several pathogens. Preformed expression of defense is often observed but does not necessarily lead to detrimental effects. Although examples of gene pyramiding are scarce, they suggest that this is a very promising strategy. More field evaluation of the transgenic plants is required to draw final conclusions on the usefulness of these genes for improving disease resistance.

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Section: Conclusion and Recommendations For Future Transgenic Approamentioning

confidence: 99%

Potential Candidate Genes for Improving Rice Disease Resistance

Delteil

Zhang²,

Lessard³

et al. 2010

Rice

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“…Several members have been studied for their functions potentially related to stress responses, such as LIP19 (Shimizu et al, 2005), OsBZ8 (Nakagawa et al, 1996;Mukherjee et al, 2006), and RF2a and RF2b (Dai et al, 2004(Dai et al, , 2008. Noticeably, in contrast to the comprehensive studies of ABFs in Arabidopsis, mainly three members of the third subfamily in rice, TRAB1, OsABI5, and OsbZIP23, have been studied for their roles in ABA-mediated stress responses (Hobo et al, 1999;Xiang et al, 2008;Zou et al, 2008).…”

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confidence: 99%

Constitutive Activation of Transcription Factor OsbZIP46 Improves Drought Tolerance in Rice

et al. 2012

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OsbZIP46 is one member of the third subfamily of bZIP transcription factors in rice (Oryza sativa). It has high sequence similarity to ABA-responsive element binding factor (ABF/AREB) transcription factors ABI5 and OsbZIP23, two transcriptional activators positively regulating stress tolerance in Arabidopsis (Arabidopsis thaliana) and rice, respectively. Expression of OsbZIP46 was strongly induced by drought, heat, hydrogen peroxide, and abscisic acid (ABA) treatment; however, it was not induced by salt and cold stresses. Overexpression of the native OsbZIP46 gene increased ABA sensitivity but had no positive effect on drought resistance. The activation domain of OsbZIP46 was defined by a series of deletions, and a region (domain D) was identified as having a negative effect on the activation. We produced a constitutive active form of OsbZIP46 (OsbZIP46CA1) with a deletion of domain D. Overexpression of OsbZIP46CA1 in rice significantly increased tolerance to drought and osmotic stresses. Gene chip analysis of the two overexpressors (native OsbZIP46 and the constitutive active form OsbZIP46CA1) revealed that a large number of stress-related genes, many of them predicted to be downstream genes of ABF/ AREBs, were activated in the OsbZIP46CA1 overexpressor but not (even down-regulated) in the OsbZIP46 overexpressor. OsbZIP46 can interact with homologs of SnRK2 protein kinases that phosphorylate ABFs in Arabidopsis. These results suggest that OsbZIP46 is a positive regulator of ABA signaling and drought stress tolerance of rice depending on its activation. The stress-related genes activated by OsbZIP46CA1 are largely different from those activated by the other rice ABF/AREB homologs (such as OsbZIP23), further implying the value of OsbZIP46CA1 in genetic engineering of drought tolerance.

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“…RF2a and RF2b, two close VIP1 homologs in rice (Oryza sativa), also are involved in regulating vascular gene expression and in regulating responses to the Rice tungro virus (Yin et al, 1997;Petruccelli et al, 2001;Dai et al, 2004Dai et al, , 2006Dai et al, , 2008. RSG, a close VIP1 homolog in tobacco (Nicotiana tabacum), regulates the biosynthesis of a phytohormone, GA (Fukazawa et al, 2000(Fukazawa et al, , 2011Igarashi et al, 2001;Ishida et al, 2004Ishida et al, , 2008Ito et al, 2010Ito et al, , 2014.…”

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confidence: 99%

The bZIP protein VIP1 is involved in touch responses in Arabidopsis roots

Tsugama¹,

Liu²,

Takano³

2016

Plant Physiol.

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VIP1 is a bZIP transcription factor in Arabidopsis (Arabidopsis thaliana). VIP1 transiently accumulates in the nucleus when cells are exposed to hypoosmotic conditions, but its physiological relevance is unclear. This is possibly because Arabidopsis has approximately 10 close homologs of VIP1 and they function redundantly. To examine their physiological roles, transgenic plants overexpressing a repression domain-fused form of VIP1 (VIP1-SRDXox plants), in which the gene activation mediated by VIP1 is expected to be repressed, were generated. Because hypoosmotic stress can mimic mechanical stimuli (e.g. touch), the touchinduced root-waving phenotypes and gene expression patterns in those transgenic plants were examined. VIP1-SRDXox plants exhibited more severe root waving and lower expression of putative VIP1 target genes. The expression of the VIP1-green fluorescent protein (GFP) fusion protein partially suppressed the VIP1-SRDX-induced increase in root waving when expressed in the VIP1-SRDXox plants. These results suggest that VIP1 can suppress the touch-induced root waving. The VIP1-SRDX-induced increase in root waving was also suppressed when the synthetic auxin 2,4-dichlorophenoxy acetic acid or the ethylene precursor 1-aminocyclopropane-1-carboxylic acid, which is known to activate auxin biosynthesis, was present in the growth medium. Root cap cells with the auxin marker DR5rev::GFP were more abundant in the VIP1-SRDXox background than in the wild-type background. Auxin is transported via the root cap, and the conditions of outermost root cap layers were abnormal in VIP1-SRDXox plants. These results raise the possibility that VIP1 influences structures of the root cap and thereby regulates the local auxin responses in roots.

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Transgenic rice plants that overexpress transcription factors RF2a and RF2b are tolerant to rice tungro virus replication and disease

Cited by 62 publications

References 20 publications

Potential Candidate Genes for Improving Rice Disease Resistance

Potential Candidate Genes for Improving Rice Disease Resistance

Constitutive Activation of Transcription Factor OsbZIP46 Improves Drought Tolerance in Rice

The bZIP protein VIP1 is involved in touch responses in Arabidopsis roots

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