Transgenic Potato Virus Y Resistance in Potato: Evidence for an RNA-Mediated Cellular Response

Smith, Holly A.; Powers, H. R.; Swaney, S; Brown, Chuck; Dougherty, William G.

doi:10.1094/phyto-85-864

Cited by 52 publications

(20 citation statements)

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“…The presented results well agree with those obtained for RNA virus resistant plants by many other groups , Lindbo & Dougherty 1993, Sijen et al 1996, SilvaRosales et al 1994. They also very well fit to the post-transcriptional gene silencing models proposed last few years by Smith (Smith et al 1995) and .…”

Section: Genes Directed Against Insectssupporting

confidence: 71%

Transgenes in plants: protection against viruses and insects

1997

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Protective transgenes introduced into plants can be classified as directed against insects, fungi, bacteria or viruses. Mechanisms by which they protect plants in some cases are relatively simple and understood while in most cases they present only the field of rapidly progressing investigations. A brief review of the recent concepts of the resistance induced in plants against viruses by virus-derived transgenes is presented with emphasising the RNA mediated resistance. The RNA mediated resistance seems to operate in Nicotiana benthamiana plants transformed in our laboratory with cDNA of the PPV CP gene: both translatable and tmtranslatable versions of the cDNA made the transformed plants resistant against PPV. The resistant plants contained more than one copy of the transgene. To protect against insects plants were in our laboratory transformed with potato proteinase inhibitor II gene (PPI-I/). The PPI-II gene expressed in model plants inhibited trypsin activity to an expected level. List of abbreviations: PPI-II -potato proteinase inhibitor II, P C R -polymerase chain reaction, PPVplum pox virus, CP -coat protein It is n o w well established that by introducing of some extra genes into a plant g e n o m e new plant lines can be created that show an increased resistance against various pathogenes. The genes that confer resistant plant phenotypes are in this review n a m e d ,,protective" genes. The ways by which they protect plants in some instances are relatively simple and self-evident, while in others induce natural defence systems in plants or make specific use of the existing mechanisms of the gene expression control. In this communication only genes directed against insects and viruses will be considered.

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Section: Genes Directed Against Insectssupporting

confidence: 71%

Transgenes in plants: protection against viruses and insects

1997

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“…Most of our susceptible transgenic plants have a single or many (more than eight) bands when examined in DMA gel blot hybridization studies (Smith et al, 1995;S. Swaney and W. G. Dougherty, unpublished data).…”

Section: Discussionmentioning

confidence: 94%

Genetic and Biochemical Dissection of Transgenic RNA-Mediated Virus Resistance

Goodwin¹,

Chapman²,

Swaney³

et al. 1996

The Plant Cell

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“…The CP transgenic papaya lines were washed out while the RP transgenic papaya lines were kept down during screening, which has been reported by other scientists (Carr et al, 1994;Smith et al, 1995;Brederode et al, 1995;Guo et al, 2001). The growth of transgenic papaya plants showed that the RP transgenic papaya lines had overcome the harm of PRSV and made the perennial fruit crop not only resume growth for many years but also bear more fruits with higher quality.…”

Section: Discussionmentioning

confidence: 85%

Analysis on virus resistance and fruit quality for T4 generation of transgenic papaya

Wei

Lan

et al. 2007

Front. Biol. China

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Molecular biological characterization, fruit characters, and nutrients were analyzed for T4 generation of transgenic papaya. All transgenic papaya plants with the mutated replicase (RP) gene from papaya ringspot virus (PRSV) showed high resistance or immunity against PRSV in the field. The RP transgene can be steadily inherited to, and expressed at RNA level, the progenies. The growth characteristics of transgenic papaya were much better than nontransgenic papaya in the field. The non-transgenic papaya seedlings began to show typical symptoms caused by PRSV after being inoculated with PRSV. They died quickly and never grew to produce fruit. The adult trees developed yellow leaves and produced smaller fruits and were doomed to a slow death after some time, while most of transgenic papaya plants (about 91.8%) did not show any symptoms caused by PRSV, and produced more, bigger, and high quality fruits. Compared with non-transgenic plants, the fresh fruit length of T4 generation of transgenic papaya increased 2.6%-5%, and the diameter decreased 0.6%-1.5%. The flesh thickness of fresh fruit increased 12%-15%, which made it fitter for eating. Although the fresh fruit quality changed, there was no significant difference between transgenic and non-transgenic papaya. The quality characteristics of dry fruit including the contents of water, lipid, N, protein, reduced sugar, vitamin A, vitamin C, and carotene in the T4 generation of transgenic papaya were all the same as their non-transgenic parents. This means that transgenic plants and non-transgenic plants are substantially equivalent, and the transgene has no effect on dry fruit quality. In this study, we found that vitamin A and vitamin C in red-fleshed papaya were 1.4-1.8 and 1.78-2.07 times more than the yellow-fleshed ones, respectively, while N and protein were only 84.2%-92.1% and 82.1%-98.9% of the yellow-fleshed ones.

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Transgenic Potato Virus Y Resistance in Potato: Evidence for an RNA-Mediated Cellular Response

Cited by 52 publications

References 0 publications

Transgenes in plants: protection against viruses and insects

Transgenes in plants: protection against viruses and insects

Genetic and Biochemical Dissection of Transgenic RNA-Mediated Virus Resistance

Analysis on virus resistance and fruit quality for T4 generation of transgenic papaya

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