Transgenic Potato Plants Overexpressing SOD and APX Exhibit Enhanced Lignification and Starch Biosynthesis with Improved Salt Stress Tolerance

Shafi, Amrina; Pal, Awadhesh Kumar; Sharma, Vishal; Kalia, Sonika; Kumar, Sanjay; Ahuja, Paramvir Singh; Singh, Anil Kumar

doi:10.1007/s11105-017-1041-3

Cited by 74 publications

(32 citation statements)

References 68 publications

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“…Thus, transgenic plants can be engineered to enhance stress tolerance and the activities of antioxidant enzymes. An overview of transgenic plants with enhanced activities of antioxidant defense systems under several stresses is presented in [265]. Transgenic tobacco exhibited tolerance to heavy metals and overexpression of SbMYB15 enhanced the activities of CAT and SOD, also increasing the expression of MnSOD at 100 µM (2-fold) and 300 µM (3-fold) of CdCl 2 , as well as CAT1 by 62-and 9-fold at 100 and 300 µM of CdCl 2 [266].…”

Section: The Transgenic Approach In Enhancing Antioxidant Defense In mentioning

confidence: 99%

“…Transgenic plants demonstrated enhanced activities of SOD and APX in dual transgenic plants (DTP). Superoxide dismutase and APX genes may serve as a positive regulator to increased salt tolerance by the regulation of ROS and lignin biosynthesis signaling pathways in transgenic plants [ 265 ]. Transgenic tobacco exhibited tolerance to heavy metals and overexpression of SbMYB15 enhanced the activities of CAT and SOD, also increasing the expression of MnSOD at 100 μM (2-fold) and 300 μM (3-fold) of CdCl 2 , as well as CAT1 by 62- and 9-fold at 100 and 300 μM of CdCl 2 [ 266 ].…”

Section: The Transgenic Approach In Enhancing Antioxidant Defensementioning

confidence: 99%

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Reactive Oxygen Species and Antioxidant Defense in Plants under Abiotic Stress: Revisiting the Crucial Role of a Universal Defense Regulator

et al. 2020

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Global climate change and associated adverse abiotic stress conditions, such as drought, salinity, heavy metals, waterlogging, extreme temperatures, oxygen deprivation, etc., greatly influence plant growth and development, ultimately affecting crop yield and quality, as well as agricultural sustainability in general. Plant cells produce oxygen radicals and their derivatives, so-called reactive oxygen species (ROS), during various processes associated with abiotic stress. Moreover, the generation of ROS is a fundamental process in higher plants and employs to transmit cellular signaling information in response to the changing environmental conditions. One of the most crucial consequences of abiotic stress is the disturbance of the equilibrium between the generation of ROS and antioxidant defense systems triggering the excessive accumulation of ROS and inducing oxidative stress in plants. Notably, the equilibrium between the detoxification and generation of ROS is maintained by both enzymatic and nonenzymatic antioxidant defense systems under harsh environmental stresses. Although this field of research has attracted massive interest, it largely remains unexplored, and our understanding of ROS signaling remains poorly understood. In this review, we have documented the recent advancement illustrating the harmful effects of ROS, antioxidant defense system involved in ROS detoxification under different abiotic stresses, and molecular cross-talk with other important signal molecules such as reactive nitrogen, sulfur, and carbonyl species. In addition, state-of-the-art molecular approaches of ROS-mediated improvement in plant antioxidant defense during the acclimation process against abiotic stresses have also been discussed.

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Section: The Transgenic Approach In Enhancing Antioxidant Defense In mentioning

confidence: 99%

Section: The Transgenic Approach In Enhancing Antioxidant Defensementioning

confidence: 99%

Reactive Oxygen Species and Antioxidant Defense in Plants under Abiotic Stress: Revisiting the Crucial Role of a Universal Defense Regulator

et al. 2020

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“…Copper, zinc superoxide dismutase (CuZnSOD) and ascorbate peroxidase (APX) overexpression together enhance the tolerance of sweet potato under salt stress 8 . Overexpressing SOD and APX improves salt stress tolerance 9 . Therefore, studying the physiological processes and molecular genetic mechanisms associated with salt stress resistance will certainly help clarify the complex biological responses of plants to high salinity and contribute to the genetic engineering of stress-resistant plants.…”

Section: Introductionmentioning

confidence: 99%

iTRAQ-based quantitative proteomic analysis of salt stress in Spica Prunellae

Liu

Zou

Chen

et al. 2019

Sci Rep

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Spica Prunellae is an important Chinese herbal medicine. Because of its good curative effect on various diseases, this herb is consumed in large quantities in clinical applications. The metabolites of Spica Prunellae are known to change under salt stress; however, the difference in protein levels of Spica Prunellae between saline and normal conditions is unclear. In this study, we used proteomics techniques to identify differentially expressed proteins in Spica Prunellae under different saline conditions. (iTRAQ) MS/MS was used to detect statistically significant changes in protein between salt stress and normal conditions. Ultimately, we detected 1,937 proteins, 89 of which were detected in two different comparison. Based on GO, STRING and KEGG analyses, 35 significantly differentially expressed proteins were selected for further analysis. The results of functional and signal pathway analyses indicated that the cellular protein and carbohydrate metabolism of Spica Prunellae was weaker, calcium ion transport was higher, photosynthesis was higher, and protein production was faster under saline conditions than under normal conditions. This study provides useful information for studying the causes of differences in secondary metabolites in Spica Prunellae under salt stress and the protein mechanisms related to their quality.

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“…Else, there might be some unknown cis-acting elements within the promoter region that have not yet been identified or their functions not known. Higher expression noticed in the PgApx pro transgenic plants could be because of increased Apx activity with the elevation in the level of stress as was also noticed earlier in diverse plant species [21,61,62]. Halder et al [63] demonstrated that dehydrin (SbDhn2) driven under the influence of CaMV35 s pro confers tolerance to oxidative damage caused by different abiotic stresses.…”

Section: Discussionmentioning

confidence: 59%

“…APX is a potential candidate in eliminating H 2 O 2 and other reactive oxygen species (ROS). Simultaneous overexpression of SOD and APX has been shown to enable the plants to withstand severe saline conditions [21]. Increased levels of APX from Pisum sativum in transgenic tomato plants play a significant role in ameliorating oxidative stress activated upon chilling and salt stresses [22].…”

Section: Introductionmentioning

confidence: 99%

Isolation and functional characterization of three abiotic stress-inducible (Apx, Dhn and Hsc70) promoters from pearl millet (Pennisetum glaucum L.)

et al. 2019

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Pearl millet is a C 4 cereal crop that grows in arid and semi-arid climatic conditions with the remarkable abiotic stress tolerance. It contributed to the understanding of stress tolerance not only at the physiological level but also at the genetic level. In the present study, we functionally cloned and characterized three abiotic stress-inducible promoters namely cytoplasmic Apx1 (Ascorbate peroxidase), Dhn (Dehydrin), and Hsc70 (Heat shock cognate) from pearl millet. Sequence analysis revealed that all three promoters have several cis-acting elements specific for temporal and spatial expression. PgApx pro, PgDhn pro and PgHsc70 pro were fused with uidA gene in Gateway-based plant transformation pMDC164 vector and transferred into tobacco through leaf-disc method. While PgApx pro and PgDhn pro were active in seedling stages, PgHsc70 pro was active in stem and root tissues of the T 2 transgenic tobacco plants under control conditions. Higher activity was observed under high temperature and drought, and less in salt and cold stress conditions. Further, all three promoters displayed higher GUS gene expression in the stem, moderate expression in roots, and less expression in leaves under similar conditions. While RT-qPCR data showed that PgApx pro and PgDhn pro were expressed highly in high temperature, salt and drought, PgHsc70 pro was fairly expressed during high temperature stress only. Histochemical and RT-qPCR assays showed that all three promoters are inducible under abiotic stress conditions. Thus, these promoters appear to be immediate candidates for developing abiotic stress tolerant crops as these promoter-driven transgenics confer high degree of tolerance in comparison with the wild-type (WT) plants.

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Transgenic Potato Plants Overexpressing SOD and APX Exhibit Enhanced Lignification and Starch Biosynthesis with Improved Salt Stress Tolerance

Cited by 74 publications

References 68 publications

Reactive Oxygen Species and Antioxidant Defense in Plants under Abiotic Stress: Revisiting the Crucial Role of a Universal Defense Regulator

Reactive Oxygen Species and Antioxidant Defense in Plants under Abiotic Stress: Revisiting the Crucial Role of a Universal Defense Regulator

iTRAQ-based quantitative proteomic analysis of salt stress in Spica Prunellae

Isolation and functional characterization of three abiotic stress-inducible (Apx, Dhn and Hsc70) promoters from pearl millet (Pennisetum glaucum L.)

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