Transgenic plants that express genes including the 3′ untranslated region of the turnip yellow mosaic virus (TYMV) genome are partially protected against TYMV infection

Zaccomer, Bruno; Cellier, Françoise; Boyer, Jean-Christophe; Haenni, Anne‐Lise; Tepfer, Mark

doi:10.1016/0378-1119(93)90451-8

Cited by 44 publications

(19 citation statements)

References 29 publications

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“…A 100 bp noncoding terminal sequence from the turnip yellow mosaic virus (TYMV) genome was placed downstream of the chloramphenicol acetyl transferase {cat) gene and transformed into B. napus conferring partial resistance to TYMV (Zaccomer et al 1993). A. rhizogenes transformation of oilseed rape has increased TYMV resistance (Spak etal.…”

Section: Virus Resistancementioning

confidence: 99%

Genetic transformation of Brassica

Poulsen

1996

Plant Breeding

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Tissue culture regeneration of plants from Brassica species can be achieved from various explant types. Their performance, however, varies with the genotype and age of the explant and is sensitive to medium supplements and Agrobacterium cocultivation. Most Brassica transformations were carried out using Agrobacterium and it was established that nopaline and agropine strains of .4. tumefaciens and A. rhizogenes, respectively, are the most efficient. Application of virulence gene induction is ambiguous. Binary vectors are mostly apphed in spite of indications that cointegrate vectors are more efficient. Cotransformation is successful with two different bacteria or with one bacterium carrying both plasmids, however, it has proved problematic to integrate the two transgenes into separate loci. Transformations with wild type rol genes develop hairy roots, from which transgenic plants can be regenerated. Vectorless transformation is feasible with microprojectile bombardment of microspore derived embryos being the most promising.Antibiotic markers providing resistance to kanamycin, hygromycin, and spectinomycin are applicable as selectable markers, while chloramphenicol is not suitable. Among herbicides, phosphinotricin gives good results while chlorsulfuron is unsuitable for in vitro selection. Major therapeutic antibiotics being successfully applied are carbenicillin, cefotaxime, amoxycillin, and ticarcillin. Standard methods for evaluating transgenic plants also have been applied to Brassica species. Some breeding objectives obtained through genetic transformation are presented.

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Section: Virus Resistancementioning

confidence: 99%

Genetic transformation of Brassica

Poulsen

1996

Plant Breeding

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“…For the detection of CMV CP (k)-strand transcripts, 35 PCR cycles were performed following the reverse transcription step, using primers R(j) and R(k) (the latter at position 1898-1879 on CMV-R RNA3), each cycle consisting of denaturation (94 mC, 30 s), annealing (52 mC, 30 s) and extension (72 mC, 30 s). For the detection of LMV CP (k)-strand transcripts, PCR conditions using primers O(j) and O(k) (the latter at position 9868-9849 on LMV-O genomic RNA) were as described by Zaccomer et al (1993), except that the annealing temperature was 51 mC. (k)-and (j)-strand in vitro T7 transcripts of LMV CP were used as reverse transcription positive and negative controls, respectively.…”

Section: Bbccmentioning

confidence: 99%

“…Total RNA was extracted from young leaves showing systemic symptoms, treated twice with RNase-free DNase I, and used in RT-PCR experiments as described by Zaccomer et al (1993) to detect RNA complementary to the transgene mRNAs. Primers R(j) (position 1348-1365 on CMV-R RNA3) and O(j) (position 9072-9091 on LMV-O genomic RNA) used in the reverse transcription step were specific to (k)-strand transcripts from CMV-R and LMV-O transgenes respectively.…”

Section: Bbccmentioning

confidence: 99%

“…Homologous recognition was first shown to take place in plants expressing the TYMV 3h UTR when infected by TYMV (Zaccomer et al, 1993). This could favour recombination between transcripts of a viral transgene and the genome of an infecting virus (Greene & Allison, 1994), and thus it is now generally suggested to delete the 3h UTR from the viral transgene in order to reduce the frequency of recombination (Greene & Allison, 1996).…”

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Synthesis of (−)-strand RNA from the 3′ untranslated region of plant viral genomes expressed in transgenic plants upon infection with related viruses

Teycheney¹,

Aaziz

Dinant³

et al. 2000

Journal of General Virology

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Potyviruses and cucumoviruses are agronomically important groups of (j)-stranded plant RNA viruses, but they are strikingly different both structurally and in their modes of expression and replication. The potyviruses (Potyviridae) are members of the Picorna-associated supergroup, and have a monopartite, polyadenylated RNA genome, which is covalently linked to a small peptide (VPg) at its 5h end. The viral RNA is encapsidated in flexuous rod-shaped particles, and encodes a single polyprotein which is co-and post-translationally processed by three virus-encoded proteases (Dougherty & Semler, 1993). Cucumoviruses (Bromoviridae) are members of the Alpha-associated supergroup, and their genome is composed of one monocistronic and two bicistronic RNAs, which are encapsidated in icosahedral particles. The 5h

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“…In TYMV it was shown that parts of the minus-strand promoter elements are located within the 3h-terminal 38 nucleotides of the gRNA (Gargouri-Bouzid et al, 1991), which encompasses this pseudoknot structure. Further, transgenic rapeseed plants expressing high levels of a sense transcript containing the 3h-terminal 100 nucleotides of the TYMV noncoding region showed partial protection against infection by TYMV RNA or virions (Zaccomer et al, 1993). The pseudoknot structure has been shown to be important for virus replication in the case of TYMV (Deiman et al, 1997 ;Singh & Dreher, 1997) and brome mosaic bromovirus (BMV) (Lahser et al, 1993).…”

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Interference with Physalis mottle tymovirus replication and coat protein synthesis by transcripts corresponding to the 3'-terminal region of the genomic RNA--role of the pseudoknot structure.

Ranjith-Kumar

Haenni

Savithri

1998

Journal of General Virology

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The role of the 3h noncoding (NC) region of Physalis mottle tymovirus genomic RNA in the multiplication of the virus was examined using an in vivo protoplast assay system. Coat protein (CP) synthesis was specifically inhibited by sense 3h NC region transcripts. To establish the role of the pseudoknot structure present in the NC region in virus multiplication, four site-specific mutants, two of which disrupted the pseudoknot structure while the other two restored the structure, were constructed. Interestingly, none of the four sense mutant transcripts inhibited CP synthesis, suggesting that the specific sequence representing the 3h terminal pseudoknot structure may play an important role in virus multiplication. However, the wild-type antisense 3h NC transcript as well as the four antisense mutant transcripts inhibited CP synthesis, suggesting that the inhibitions by antisense transcripts could be due to the formation of RNA-RNA hybrids at the 3h end of the genomic RNA.Physalis mottle tymovirus (PhMV) belongs to the tymovirus group of plant viruses, which contain a single positivesense RNA genome of approximately 6 kb. As demonstrated for the type member, turnip yellow mosaic virus (TYMV), the 5h end of the tymoviral genome is capped (Briand et al., 1978) while the 3h end has a tRNA-like structure (TLS), which can be aminoacylated in vitro Yot et al., 1970) and in vivo (Joshi et al., 1982). Two extensively overlapping reading frames separated by 7 nucleotides code for the overlapping protein and the replicase protein (RdRp) in tymoviruses (Morch et al

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Transgenic plants that express genes including the 3′ untranslated region of the turnip yellow mosaic virus (TYMV) genome are partially protected against TYMV infection

Cited by 44 publications

References 29 publications

Genetic transformation of Brassica

Genetic transformation of Brassica

Synthesis of (−)-strand RNA from the 3′ untranslated region of plant viral genomes expressed in transgenic plants upon infection with related viruses

Interference with Physalis mottle tymovirus replication and coat protein synthesis by transcripts corresponding to the 3'-terminal region of the genomic RNA--role of the pseudoknot structure.

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