Transgenic Plant Production from Embryogenic Callus of Sweet Potato (Ipomoea batatas (L.) Lam.) Using Agrobacterium tumefaciens

Otani, Motoyasu; Shimada, Takehiko; Kimura, Takashi; Saito, Akira

doi:10.5511/plantbiotechnology.15.11

Cited by 48 publications

(46 citation statements)

References 10 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The plasmids were introduced into Agrobacterium tumefaciens strain EHA101, and used to transform embryogenic calli induced from shoot meristems as described by Otani et al (1998) with some modification: Agrobacterium was co-cultured at 23 C and on the medium containing 3% (w/v) maltose and 1% (w/v) glucose instead of 3% (w/v) sucrose.…”

Section: Transformationmentioning

confidence: 99%

Increase of amylose content of sweetpotato starch by RNA interference of the starch branching enzyme II gene (IbSBEII)

Shimada

Otani

Hamada

et al. 2006

Plant Biotechnology

Self Cite

View full text Add to dashboard Cite

In the storage roots of sweetpotato (Ipomoea batatas (L.) Lam. cv. Kokei 14), 10 to 20% of the starch is essentially unbranched linear amylose and the other major component is branched amylopectin. The starch branching enzymes, which are responsible for production of amylopectin to form a-1,6-linkages in the glucan can be divided into two classes, class A (e.g. potato and maize SBEII, pea SBEI) and class B (e.g. potato and maize SBEI, pea SBEII). On the bases of the registered cDNA of sweetpotato SBEII (IbSBEII) encoding class A branching enzyme, we constructed doublestranded RNA (dsRNA) interference vectors and introduced them into sweetpotato genome via Agrobacterium-mediated gene transformation. We obtained eight independent transgenic plants by using two kinds of RNA interference (RNAi) constructs, encoding GBSSI 1st intron-spliced RNA or a GUS fragment-spliced RNA, respectively. All transgenic plants were confirmed not to express IbSBEII by RT-PCR and to have the starch with a higher amylose content than the nontransgenic control (up to 25% compared to 10% in the control). Both constructs induced the same level of silencing of IbSBEII in all transgenic plants. The morphological characters showed no significant differences between the transgenic and control plants. Starch yield of transgenic tubers was slightly lower than that of non-transgenic tubers. The starch granules of the transgenic plants were similar to those of typical sweetpotato starchs in shape and the distribution in granule size, but slightly different in grain structure.

show abstract

Section: Transformationmentioning

confidence: 99%

Increase of amylose content of sweetpotato starch by RNA interference of the starch branching enzyme II gene (IbSBEII)

Shimada

Otani

Hamada

et al. 2006

Plant Biotechnology

Self Cite

View full text Add to dashboard Cite

show abstract

“…To date, several reports on the Agrobacterium-mediated transformation of sweet potato have been published. However majority of the most efficient and robust Agrobacterium transformation systems succeed in transformation of marker genes such as gfp, gusA, nptII, bar [5,10,[34][35][36][37]. Therefore, genetic transformation of sweet potato remains cumbersome and labor-intensive due to the difficulty in plant regeneration of several recalcitrant cultivars, and the reproducibility of the used protocol [15,17,34,[36][37][38][39] .…”

Section: Biological Test Resultsmentioning

confidence: 99%

Agrobacterium-Mediated Transformation of Cry8db Gene in Vietnam Sweet Potato Cultivar

Ngoc¹,

Lan²,

Trang³

et al. 2015

JLS

View full text Add to dashboard Cite

Sweet potato [Ipomoea batatas (L.) Lam.] is an important food crop in the world as well as in Vietnam. It is well known as a recalcitrant crop for gene transformation and tissue culture because of its genotype dependent in vitro responses. In present study, Agrobacterium-mediated transformation of cry8Db from Bacillus thuringiensis into KB1 sweet potato variety has been studied. The C58cv strain carrying a pBI121 backbone which contained cry8Db delta-endotoxin gene regulated under 35S CaMV promoter, and the selection marker gene, neomycin phosphotransferase (nptII) gene, was subjected for plant transformation. Callus induced from shoot tips and leaf explants were inoculated and cocultured with A. tumefaciens. The selection occurred during callus producing and plant regenerating steps. A total of 201 transgenic putative plant lines were produced, and 21 transgenic lines were positively confirmed by PCR and finalized by Southern blot. Four putative transgenic lines confirming a single copy of the cry8Db gene were transferred into soil pots in greenhouse. Biological activity evaluation for the insecticidal capacity of these transgenic lines under controlled conditions showed that the level of infestation by sweet potato weevils (Cylas formicarius) in untransformed plants was higher than that of transgenic lines.

show abstract

“…As shown in Figure 3a, although there was no significant difference between 3 and 4 days of cocultivation for M. zumi callus, 4 days of co-cultivation caused overgrowth of A. tumefaciens which were difficult to completely eliminate by antibiotic and therefore were harmful to the callus. Prakash et al (1991) indicated that the co-cultivation period should be not less than 4 days, and Otani et al (1998) indicated that 2 days was suggested for co-cultivation period. Xing et al (2007) maintained that the difference in co-cultivation period was due to the different species and explants.…”

Section: Optimization Of Conditions For Transformation and Plant Regementioning

confidence: 99%

Efficient Agrobacterium tumefaciens-mediated transformation of Malus zumi (Matsumura) Rehd using leaf explant regeneration system

Xu¹,

Wang²,

Yin³

et al. 2009

Electron. J. Biotechnol.

View full text Add to dashboard Cite

Transgenic Plant Production from Embryogenic Callus of Sweet Potato (Ipomoea batatas (L.) Lam.) Using Agrobacterium tumefaciens

Cited by 48 publications

References 10 publications

Increase of amylose content of sweetpotato starch by RNA interference of the starch branching enzyme II gene (IbSBEII)

Increase of amylose content of sweetpotato starch by RNA interference of the starch branching enzyme II gene (IbSBEII)

Agrobacterium-Mediated Transformation of Cry8db Gene in Vietnam Sweet Potato Cultivar

Efficient Agrobacterium tumefaciens-mediated transformation of Malus zumi (Matsumura) Rehd using leaf explant regeneration system

Contact Info

Product

Resources

About