Transgenic Plant Cell Cultures: A Promising Approach for Secondary Metabolite Production

Satish, Lakkakula; Rency, Arockiam Sagina; Muthubharathi, Balasubramanian Chellammal; Shamili, Sasanala; Ramesh, Manikandan; Swamy, Mallappa Kumara

doi:10.1007/978-981-13-7438-8_4

Cited by 9 publications

(5 citation statements)

References 328 publications

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“…( Ochoa‐Villarreal et al., 2016 ; Georgiev et al., 2018 ). Because of its ability to minimize wrinkles in the crow’s feet area of the face, a liposome-encapsulated extract of cultured apple stem cells is employed as an ideal ingredient in anti-aging products ( Satish et al., 2019 ; Krasteva et al., 2020 ; Krol et al., 2020 ). Chemicals isolated from Catharanthusroseus are currently used in the manufacturing of both ordinary consumer and professional care cosmetics, demonstrating significant potential in protecting skin from heavy metal toxicity ( Mallurwar and Pathak, 2008 ; Schmid and Zülli, 2012 ; Blum et al., 2013 ; Hayta et al., 2017 ; Meir et al., 2018 ).…”

Section: Applications Of Plant Tissue Culturementioning

confidence: 99%

Plants in vitro propagation with its applications in food, pharmaceuticals and cosmetic industries; current scenario and future approaches

et al. 2022

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Plant tissue culture technique employed for the identification and isolation of bioactive phytocompounds has numerous industrial applications. It provides potential benefits for different industries which include food, pharmaceutical and cosmetics. Various agronomic crops i.e., cereals, fruits, vegetables, ornamental plants and forest trees are currently being used for in vitro propagation. Plant tissue culture coupled with biotechnological approaches leads towards sustainable agricultural development providing solutions to major food security issues. Plants are the rich source of phytochemicals with medicinal properties rendering them useful for the industrial production of pharmaceuticals and nutraceuticals. Furthermore, there are numerous plant compounds with application in the cosmetics industry. In addition to having moisturizing, anti‐ageing, anti‐wrinkle effects; plant-derived compounds also possess pharmacological properties such as antiviral, antimicrobial, antifungal, anticancer, antioxidant, anti-inflammatory, and anti-allergy characteristics. The in vitro propagation of industrially significant flora is gaining attention because of its several advantages over conventional plant propagation methods. One of the major advantages of this technique is the quick availability of food throughout the year, irrespective of the growing season, thus opening new opportunities to the producers and farmers. The sterile or endangered flora can also be conserved by plant micro propagation methods. Hence, plant tissue culture is an extremely efficient and cost-effective technique for biosynthetic studies and bio-production, biotransformation, or bioconversion of plant-derived compounds. However, there are certain limitations of in-vitro plant regeneration system including difficulties with continuous operation, product removal, and aseptic conditions. For sustainable industrial applications of in-vitro regenerated plants on a large scale, these constraints need to be addressed in future studies.

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Section: Applications Of Plant Tissue Culturementioning

confidence: 99%

Plants in vitro propagation with its applications in food, pharmaceuticals and cosmetic industries; current scenario and future approaches

et al. 2022

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“…These results shown that radical scavenging activity of A. longiloba extracts could be mostly related to their TPC. The anti-free-radicals property of phenolic compounds depends on their molecular structures, particularly the availability of phenolic hydrogens leads to the formation of phenoxyl radicals by hydrogen donation and confers to phenolic compounds the ability of in-activating free radicals [ 54 , 55 , 56 ]. Using the DPPH assay, the antioxidant activities of A. longiloba field-grown plant parts and in vitro-derived callus extract were ranked in the order in vitro-derived callus > petiole > fruit.…”

Section: Resultsmentioning

confidence: 99%

Micropropagation of Alocasia longiloba Miq and Comparative Antioxidant Properties of Ethanolic Extracts of the Field-Grown Plant, In Vitro Propagated and In Vitro-Derived Callus

Abdulhafiz

Mohammed

Kayat

et al. 2020

Plants

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In this study, an efficient micropropagation protocol was developed for A. longiloba and the antioxidant properties of field-grown plant, in vitro-derived greenhouse-grown plant and in vitro-derived callus extracts were compared. The A. longiloba seeds tested using tetrazolium chloride salt exhibited 89% viability. Due to poor germination capacity of A. longiloba seeds, the seeds were treated with gibberellic acid (GA3) or sulfuric acid (H2SO4). The maximum seed germination of 87% was observed at 30% H2SO4 treatment after 19.00 d, whereas GA3 treatment showed maximum germination of 53% after 22 d. In vitro shoot multiplication was carried out using various types of cytokinins alone or in combination with auxin. Among them, 6-benzyl amino purine (BAP) single treatment was found to be the best hormone. The highest shoot-length (7.26 cm) and maximum number of shoots per explant (18) were recorded at 3-mg L−1 BAP. For in vitro rooting, indole-3-acetic acid at 0.5-mg L−1 was found to be the optimum concentration. Callus was induced using various types of auxins alone or in combinations with cytokinins. The highest percentage of callus of 91 and fresh weight of 6 g was obtained with 3-mg L−1 IAA. The plantlets produced in the current study were subjected to acclimatization. The combination of topsoil and peat moss at 1:2 ratio was found to be the best soil media. In this study, in vitro-derived callus extract showed the highest phenolic content (538 mg GAE), followed by extracts of field-grown plant parts, i.e., fruit and petiole (504 and 300 mg GAE) while in vitro plant extract showed the lowest (98 mg GAE). Meanwhile, the highest flavonoids was recorded in petiole extract. Comparative antioxidant activity study shows, in vitro-derived callus exhibited better DPPH-radical-scavenging activity (IC50: 0.113-mg mL−1) whereas the extracts of petiole, fruit and in vitro plant showed 0.126-, 0.137- and 0.173-mg mL−1, respectively. At the same time, the fruit extract showed better (IC50: 0.088-mg mL−1) ABTS radical scavenging activity than all extracts tested. In conclusion, the in vitro-derived callus extract could be favored for high TPC and better DPPH scavenging activity. Hence, the present study was conducted to establish an efficient micropropagation protocol and to compare the antioxidant activity of the field-grown plant, in vitro plant and in vitro derived callus extracts of A. longiloba.

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“…Plant genetic transformation has a very wide application in expression of recombinant proteins [44]; these can have many therapeutic or diagnostic uses, increase plant resistance to biotic and abiotic stresses [45] or increase their nutritional or taste values [46]. Another approach is known as metabolic engineering: it can be used to increase the production of selected metabolites that are naturally synthesized in their tissues, or for the synthesis of completely new compounds [47,48].…”

Section: From Natural Gene Transfer To Plant Metabolic Engineeringmentioning

confidence: 99%

Transgenesis as a Tool for the Efficient Production of Selected Secondary Metabolites from Plant in Vitro Cultures

Kowalczyk

Wieczfińska

Skała

et al. 2020

Plants

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The plant kingdom abounds in countless species with potential medical uses. Many of them contain valuable secondary metabolites belonging to different classes and demonstrating anticancer, anti-inflammatory, antioxidant, antimicrobial or antidiabetic properties. Many of these metabolites, e.g., paclitaxel, vinblastine, betulinic acid, chlorogenic acid or ferrulic acid, have potential applications in medicine. Additionally, these compounds have many therapeutic and health-promoting properties. The growing demand for these plant secondary metabolites forces the use of new green biotechnology tools to create new, more productive in vitro transgenic plant cultures. These procedures have yielded many promising results, and transgenic cultures have been found to be safe, efficient and cost-effective sources of valuable secondary metabolites for medicine and industry. This review focuses on the use of various in vitro plant culture systems for the production of secondary metabolites.

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Transgenic Plant Cell Cultures: A Promising Approach for Secondary Metabolite Production

Cited by 9 publications

References 328 publications

Plants in vitro propagation with its applications in food, pharmaceuticals and cosmetic industries; current scenario and future approaches

Plants in vitro propagation with its applications in food, pharmaceuticals and cosmetic industries; current scenario and future approaches

Micropropagation of Alocasia longiloba Miq and Comparative Antioxidant Properties of Ethanolic Extracts of the Field-Grown Plant, In Vitro Propagated and In Vitro-Derived Callus

Transgenesis as a Tool for the Efficient Production of Selected Secondary Metabolites from Plant in Vitro Cultures

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