Transgenic perennial ryegrass (Lolium perenne) plants from microprojectile bombardment of embryogenic suspension cells

Spangenberg, Germán; Wang, Zeng-yu; Wu, Xiaolin; Nagel, J.; Potrykus, Ingo

doi:10.1016/0168-9452(95)04135-h

Cited by 108 publications

(50 citation statements)

References 29 publications

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“…The plasmid pAcH1 was used to select transformed cells (Spangenberg et al 1995). This plasmid bears a hygromycin phosphotransferase (HPT) gene under the control of a rice (Oryza sativa L.) actin 1 (Act1) promoter (McElroy et al 1990).…”

Section: Expression Vectorsmentioning

confidence: 99%

Production of transgenic Italian ryegrass expressing the betaine aldehyde dehydrogenase gene of zoysiagrass

Takahashi

Oishi²,

Ebina

et al. 2010

Breed. Sci.

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Transgenic Italian ryegrass plants expressing the betaine aldehyde dehydrogenase gene of zoysiagrass (ZBD1) were produced via particle bombardment of embryogenic calluses. Although the growth of both non-transgenic and transgenic plants expressing ZBD1 was inhibited under salt stress, isolated tillers of the transgenic plants showed better re-rooting ability in vitro in the presence of 300 mM NaCl. Chlorophyll fluorescence values of the transgenic plants declined under salt stress, but were significantly higher than those of non-transgenic plants under salt stress, indicating the improved salt stress tolerance of transgenic plants.

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Section: Expression Vectorsmentioning

confidence: 99%

Production of transgenic Italian ryegrass expressing the betaine aldehyde dehydrogenase gene of zoysiagrass

Takahashi

Oishi²,

Ebina

et al. 2010

Breed. Sci.

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“…Promoter-GUS fusions were made to localize the activity of the selected promoters in perennial ryegrass. Hygromycin selection of transformants was as described by Spangenberg et al (1995). Transgene presence in primary transformants was confirmed by polymerase chain reaction (PCR) analysis.…”

Section: Methodsmentioning

confidence: 99%

“…Three plasmids (pVDH636, pVDH743, pVDH728) containing the ATH1 cDNA were used for transformations. These plasmids contained the hygromycin phosphotransferase marker gene driven by the rice Act promoter (Spangenberg et al 1995). The following promoters were used to drive ATH1 gene expression: (1) the maize Ubi-promoter (Christensen et al 1992), (2) the rice Actpromoter (McElroy et al 1991), and (3) the rice OSH1-promoter (Matsuoka et al 1993).…”

Section: Methodsmentioning

confidence: 99%

“…Plant material, tissue culture, growth conditions and flowering experiments: Seeds of the varieties ÔMondialÕ and ÔKelvinÕ of the outbreeding grass species L. perenne L. were used for the production of embryogenic callus as described by Spangenberg et al (1995). Extensive screening of a large number of seeds resulted in the identification of a few highly embryogenic genotypes.…”

Section: Methodsmentioning

confidence: 99%

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Late heading of perennial ryegrass caused by introducing an Arabidopsis homeobox gene

et al. 2004

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Perennial ryegrass (Lolium perenne L.) is the most important temperate forage grass species. Unfortunately, the nutritional value of perennial ryegrass declines as maturity progresses, mainly because of a high concentration of poorly digestible compounds in inflorescences. Therefore, the development of forage-type ryegrass varieties with extended vegetative growth is of interest for agriculture. To delay floral transition in perennial ryegrass the Arabidopsis ATH1 gene driven by the maize ubiquitin promoter, the rice actin promoter or the rice OSH1 promoter, respectively was introduced. In ATH1-expressing plants heading was delayed, and in a number of cases the plants never flowered at all. Such non-or late-heading was accompanied by the outgrowth of normally quiescent lateral meristems into extra leaves, resulting in a leafy growth habit. When eventually heading, these plants generally produced a reduced number of inflorescences. These observations suggest that ATH1-mediated delay of heading may be useful to improve fodder quality of perennial ryegrass.

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“…Since the first transgenic Lolium perenne (perennial ryegrass) plants were generated by Spangenberg et al (1995), efforts in Lolium transformation have resulted in successful transformation of L. multiflorum, L. perenne and L. temulentum by biolistic delivery (Ye et al 1997;Dalton et al 1999) and Agrobacterium tumefaciens-mediated methods (Bettany et al 2003;Bajaj et al 2006;Cao et al 2006;Altpeter 2006;Sato and Takamizo 2006;Ge et al 2007;Zhang et al 2012). This has resulted in progress in the development of traits such as improved digestibility (Tu et al 2010), salt tolerance (Wu et al 2005) and cold tolerance (Hisano et al 2004).…”

Section: Introductionmentioning

confidence: 99%

Agrobacterium-mediated transformation of Lolium rigidum Gaud.

Ran

Patron

et al. 2014

Plant Cell Tiss Organ Cult

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Lolium rigidum Gaud. is an annual grass grown for forage but also an economically damaging crop weed. A single genotype somatic embryogenic callus line, VLR1-60, was identified from a herbicide susceptible L. rigidum population, VLR1, and proved to be amenable to Agrobacterium tumefaciens-mediated transformation. Somatic embryogenic calli were continuously induced from the meristematic region of VLR1-60 plants multiplied in vitro and the basic tolerance level of VLR1-60 to hygromycin B was determined. A hygromycin phosphotransferase gene was used as a selectable marker for hygromycin B selection. Somatic embryogenic calli derived from in vitro grown vegetative tillers were co-cultivated with the A. tumefaciens strain EHA105 harbouring binary vector carrying reporter genes and selectable marker in the presence of acetosyringone for 3 days. Inoculated calli were recovered on callus proliferation medium containing Timentin TM but lacking hygromycin and were then subcultured onto media with hygromycin concentrations increased progressively through time for selection of transformed plant cells. Putative transgenic plants were recovered and integration of transgenes was confirmed by Southern hybridization analysis and by detection of DsRed or GUS activity in transgenic plants. The frequency of plant transformation was 1.3 %. The ability to transform L. rigidum will provide opportunities for functional characterization of genes to improve forage quality and increase our understanding of the evolution of herbicide resistance and of the basic genetics underlying traits that make L. rigidum a damaging crop weed.

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Transgenic perennial ryegrass (Lolium perenne) plants from microprojectile bombardment of embryogenic suspension cells

Cited by 108 publications

References 29 publications

Production of transgenic Italian ryegrass expressing the betaine aldehyde dehydrogenase gene of zoysiagrass

Production of transgenic Italian ryegrass expressing the betaine aldehyde dehydrogenase gene of zoysiagrass

Late heading of perennial ryegrass caused by introducing an Arabidopsis homeobox gene

Agrobacterium-mediated transformation of Lolium rigidum Gaud.

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