Transgenic Pea Seeds Expressing the α-Amylase Inhibitor of the Common Bean are Resistant to Bruchid Beetles

Shade, Richard E.; Schroeder, H. E.; Pueyo, José Javier; Tabe, Linda; Murdock, Larry L.; Higgins, Thomas J.; Chrispeels, Maarten J.

doi:10.1038/nbt0894-793

Cited by 256 publications

(149 citation statements)

References 14 publications

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“…That many defenses of legumes are proteinaceous made these plants attractive for studies of chemical coevolution in that such defenses are the direct products of protein-coding genes; moreover, such forms of resistance were attractive candidates for genetically engineering pestprotected crop plants. A substantial body of literature accumulated documenting the production of proteinase inhibitors and the counter adaptations to these defenses (Murdock et al, 1988;Chrispeels and Raikhel, 1991;Shade et al, 1994). As well, studies of lima beans (Phaseolus lunatus) and spider mites compellingly demonstrated the role of plant volatiles, including widely conserved defense signaling substances, as signals to third trophic level enemies of herbivores (Dicke et al, 1990;Dicke and Sabelis, 1990;Vet and Dicke, 1992;de Boer et al, 2005).…”

Section: 1)mentioning

confidence: 99%

Facing the Future of Plant-Insect Interaction Research: Le Retour à la “Raison d'Être”

Berenbaum

Zangerl

2008

Plant Physiology

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Section: 1)mentioning

confidence: 99%

Facing the Future of Plant-Insect Interaction Research: Le Retour à la “Raison d'Être”

Berenbaum

Zangerl

2008

Plant Physiology

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“…The recent development of procedures for genetic manipulation of peas (Pisum sativum) (Schroeder et al, 1993) provided an opportunity to test the insecticidal activity of a-AI protein against these insects in vivo. Transgenic peas that expressed the bean aai gene in their seeds at the same level as normally found in bean seeds indeed showed complete resistance to both C. maculatus and C. chinensis (Shade et al, 1994).…”

mentioning

confidence: 98%

“…Construction of the plasmid gene (pMCP3) containing the chimeric aai gene was described in detail elsewhere (Shade et al, 1994). The protein-coding region of the crai gene was equipped with 5' and 3' flanking regions from the bean PHA gene dlec2.…”

Section: Chimeric Cene Constructionmentioning

confidence: 99%

Bean [alpha]-Amylase Inhibitor Confers Resistance to the Pea Weevil (Bruchus pisorum) in Transgenic Peas (Pisum sativum L.)

et al. 1995

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Bruchid larvae cause major losses of grain legume crops throughout the world. Some bruchid species, such as the cowpea weevil and the azuki bean weevil, are pests that damage stored seeds. Others, such as the pea weevil (Bruchus pisorum), attack the crop growing i n the field. We transferred the cDNA encoding the a-amylase inhibitor (a-AI) found in the seeds of the common bean (Phaseolus vulgaris) into pea (Pisum sativum) using Agrobacferium-mediated transformation. Expression was driven by the promoter of phytohemagglutinin, another bean seed protein. The a-amylase inhibitor gene was stably expressed in the transgenic pea seeds at least to the T, seed generation, and a-AI accumulated i n the seeds up to 3% of soluble protein. This level is somewhat higher than that normally found in beans, which contain 1 to 2 % a-AI. In the 1 , seed generation the development of pea weevil larvae was blocked at an early stage. Seed damage was minimal and seed yield was not significantly reduced in the transgenic plants. These results confirm the feasibility of protecting other grain legumes such as lentils, mungbean, groundnuts, and chickpeas against a variety of bruchids using the same approach. Although a-AI also inhibits human a-amylase, cooked peas should not have a negative impact on human energy metabolism.The common bean (Phaseolus vulgaris L.) contains a family of structurally related seed proteins: PHA-E and -L, arcelin, and a-AI. PHA-E and PHA-L are strong agglutinins, i.e. classical lectins that bind carbohydrate, and arcelin, which is found only in certain wild accessions of the common bean, may be a weak agglutinin (Hartweck et al., 1991). The bean a-AI has 65 to 70% amino acid sequence identity with the other three but lacks at least one of the conserved residues needed for lectin activity. Its biochemical mode of action is to form a one-to-one complex with certain amylases (for reviews, see Chrispeels and Raikhel, 1991;Rouge et al., 1993).

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“…The bean (Phaseolus vulgaris) a-amylase inhibitor gene was expressed in seeds of transgenic garden pea (Pisum sativum) and other grain legumes, using a strong seed-specific promoter (Shade et al, 1994). The resulting seeds contained up to 3% of the foreign protein and were resistant to stored product pests, such as larvae of bruchid beetles, and field pests, such as larvae of the pea weevil Bruchus pisorum (Morton et al, 2000).…”

Section: Bean A-amylase Inhibitors and Stored Product Pestsmentioning

confidence: 99%

Biotechnological Prospects for Engineering Insect-Resistant Plants

Gatehouse

2008

Plant Physiology

191

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Insect-resistant crops have been one of the major successes of applying plant genetic engineering technology to agriculture; cotton (Gossypium hirsutum) resistant to lepidopteran larvae (caterpillars) and maize (Zea mays) resistant to both lepidopteran and coleopteran larvae (rootworms) have become widely used in global agriculture and have led to reductions in pesticide usage and lower production costs (Toenniessen et al., 2003;Brookes and Barfoot, 2005).The source of the insecticidal toxins produced in commercial transgenic plants is the soil bacterium Bacillus thuringiensis (Bt). Bt strains show differing specificities of insecticidal activity toward pests, and constitute a large reservoir of genes encoding insecticidal proteins, which are accumulated in the crystalline inclusion bodies produced by the bacterium on sporulation (Cry proteins, Cyt proteins) or expressed during bacterial growth (Vip proteins). The threedomain Cry proteins have been extensively studied; their mechanism of action involves a proteolytic activation step, which occurs in the insect gut after ingestion, followed by interaction of one or both of domains II and III with ''receptors'' on the surface of cells of the insect gut epithelium. This interaction leads to oligomerization of the protein, and domain I is then responsible for the formation of an open channel through the cell membrane . The resulting ionic leakage destroys the cell, leading to breakdown of the gut, bacterial proliferation, and insect death.However, not all pests are adequately targeted by the Bt toxins used at present, and there is still a need to develop solutions to specific problems, such as resistance to sap-sucking pests and pests of stored products. This Update will review some developments to the basic Bt strategy and selected alternative methods for engineering insect resistance.

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Transgenic Pea Seeds Expressing the α-Amylase Inhibitor of the Common Bean are Resistant to Bruchid Beetles

Cited by 256 publications

References 14 publications

Facing the Future of Plant-Insect Interaction Research: Le Retour à la “Raison d'Être”

Facing the Future of Plant-Insect Interaction Research: Le Retour à la “Raison d'Être”

Bean [alpha]-Amylase Inhibitor Confers Resistance to the Pea Weevil (Bruchus pisorum) in Transgenic Peas (Pisum sativum L.)

Biotechnological Prospects for Engineering Insect-Resistant Plants

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