Transgenic oil palm: production and projection

Parveez, Ghulam Kadir Ahmad; Masri, Masnita Mohd; Zainal, Alizah; Majid, Na'imatulapidah Abdul; Masani, Mat Yunus Abdul; Fadilah, Haliza Haris; Rasid, Omar Abdul; Cheah, S. C.

doi:10.1042/bst0280969

Cited by 33 publications

(12 citation statements)

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“…Taken together, it took nearly 2 years to regenerate transformed oil palm plantlets that were of approximately 15 cm in height from the day of transformation. Previously, Parveez et al (2000) reported that it took almost 3 years to generate Basta-resistant transgenic oil palm plantlets after transformation using biolistic. The plantlets obtained were all phenotypically normal.…”

Section: Resultsmentioning

confidence: 99%

“…Even though regeneration of transgenic oil palm from EC was first reported more than 10 years ago, the transformation efficiencies were rather low, between 0.7 and 1.5% only (Parveez et al, 2000; Masli et al, 2009). These reports used EC as target tissue and the efficiency was calculated based on percentage of transient over stable transformation.…”

Section: Introductionmentioning

confidence: 99%

“…One of the approaches to increase transformation efficiency is to evaluate selection agents used to select transformed cells from the majority of untransformed cells. Transformation of oil palm has initially been carried out using herbicide Basta, as selection agents (Parveez et al, 2000). From minimal inhibition studies, it was found that Basta and hygromycin were the most efficient selection agents for oil palm transformation (Parveez et al, 1996).…”

Section: Introductionmentioning

confidence: 99%

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Evaluation on the effectiveness of 2-deoxyglucose-6-phosphate phosphatase (DOGR1) gene as a selectable marker for oil palm (Elaeis guineensis Jacq.) embryogenic calli transformation mediated by Agrobacterium tumefaciens

et al. 2015

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DOGR1, which encodes 2-deoxyglucose-6-phosphate phosphatase, has been used as a selectable marker gene to produce transgenic plants. In this study, a transformation vector, pBIDOG, which contains the DOGR1 gene, was transformed into oil palm embryogenic calli (EC) mediated by Agrobacterium tumefaciens strain LBA4404. Transformed EC were exposed to 400 mg l-1 2-deoxyglucose (2-DOG) as the selection agent. 2-DOG resistant tissues were regenerated into whole plantlets on various regeneration media containing the same concentration of 2-DOG. The plantlets were later transferred into soil and grown in a biosafety screenhouse. PCR and subsequently Southern blot analyses were carried out to confirm the integration of the transgene in the plantlets. A transformation efficiency of about 1.0% was obtained using DOGR1 gene into the genome of oil palm. This result demonstrates the potential of using combination of DOGR1 gene and 2-DOG for regenerating transgenic oil palm.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Evaluation on the effectiveness of 2-deoxyglucose-6-phosphate phosphatase (DOGR1) gene as a selectable marker for oil palm (Elaeis guineensis Jacq.) embryogenic calli transformation mediated by Agrobacterium tumefaciens

et al. 2015

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“…Investigating methylation patterns of these target genes will pave the way for understanding the epigenetic mechanisms underlying the induction and maintenance of somaclonal variation in oil palm regenerants. Since the initial transformation of oil palm with a marker gene has been reported by Parveez et al (2000), genetic engineering in oil palm is progressing at the slow pace imposed by public acceptance, biosafety issues, and the genetic complexity of desired traits (from the production of bioplastics to the introduction of resistance to major pasts and diseases). Subhi et al (2010) have recently identified the ubiquitin extension protein (uep1) gene as a constitutively expressed gene in oil palm.…”

Section: Biotechnologies Already Impacts Oil Palm Productivitymentioning

confidence: 99%

Oil palm biotechnologies are definitely out of infancy

Rival¹,

Jaligot²

2010

OCL

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Although biotechnologies and sustainable development are often considered as antagonists, there is increasing evidence for a role for this approach in the ecological intensification of oil palm cultivation. Ecological intensification is based on the understanding of how nature functions so as to exploit its resources without destroying it. Living organisms are supported by the genome (DNA) through the action of the transcriptome (RNAs), proteome, metabolome, and ionome, the four basic pillars of functional genomics. These pillars represent the sum of all the expressed genes, proteins, metabolites, and elements within an organism. The dynamic response and interaction of these biochemical ''omes'' defines how a living system functions, and its study, systems biology, is now one of the biggest challenges in life sciences. In oil palm, as in many major crops, functional genomics is still at its beginning, although there are no reasons why oil palm should not rapidly benefit from the fast progresses generated by automated and high-throughput technologies. The success of sequencing projects on model plants has created a widespread interest in exploring the structure and expression patterns of the genome. Indeed, several institutions have now achieved the full sequencing of the oil palm genome, paving the way for the rapid evolution of various genomics-based approaches. Oil palm breeding has provided an average 1% of genetic gain per year since the early 1960s and such an impressive increase in oil yield will be maintained in future generations with a major contribution from biotechnology. Indeed, the recent adoption of biotechnological approaches has already proven very useful in major areas such as cloning of outstanding material, identity checking of progenies/mother palms, identification and characterization of genes underlying agricultural traits, etc. Phenotypic differences among individuals are partly the result of quantitative differences in transcript abundance. Using microarray technology, it is now possible to assess the abundance of many transcripts-and, indeed, of the entire known transcriptome-simultaneously. Studies which attempt to localize the genetic regulators of gene expression have been carried out in several species, including maize and eucalyptus. Microarrays have been used to determine gene expression levels in segregating populations and identify genomic regions (gene expression QTLs, or eQTLs) explaining transcript variation in co-regulated genes. Our article will focus on recent developments in various plant biotechnology areas in which applications for improving sustainability already exist or will be developed in the near future.Key words: palm technologies, oil palm genome, phenotypic differences, microarray technology, gene expression QTLs, improving sustainability Biotechnologies and sustainable development are often considered as antagonists, especially in Europe, where GMOs (Genetically Modified Organisms) and agricultural biotechnology are opposed to more environment-friendl...

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“…Various genetically modified plants are being developed, such as shorter palms that can produce fruits for longer than 25 years, but commercial plantings are unlikely before 2020 (Parveez et al 2000). By May 2008, the first phase of sequencing the oil palm genome was complete, which may provide significant future improvements in both quality and production (Crowley 2008).…”

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confidence: 99%