Transgenic Nicotiana debneyii expressing viral coat protein are resistant to potato virus S infection

MacKenzie, Donald J.; Tremaine, J. H.

doi:10.1099/0022-1317-71-9-2167

Cited by 40 publications

(10 citation statements)

References 30 publications

(27 reference statements)

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“…When plants of the same tobacco line 3404 were inoculated with naked TMV RNA at 0.5-1.0 μg/ml which could easily initiated infection on susceptible controls, failed to infect CPtransgenic plants (Reimann-Philipp and Beachy, 1993) indicating a degree of resistance to TMV RNA challenge inoculation. Similar results have also been reported in 1987 using potato viruses X or S (PVX or PVS) CP transgenic plants and PVX or PVS RNA inocula (Hemenway et al, 1988;MacKenzie and Tremaine, 1990;MacKenzie et al, 1991) (Table 3). Occasionally, an antisense CP gene construct would confer some protection against the cognate virus in transgenic test plants (Hemenway et al, 1988: Cuozzo et al, 1988.…”

Section: Pathogen-derived Resistance Strategies In Crop Protectionsupporting

confidence: 76%

Pathogen-Derived Resistance Against Plant Viruses: Postscript and Prospects

Arif¹,

Hassan²

1999

Pakistan J. of Biological Sciences

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Pathogen-derived resistance strategies using coat proteins, movement proteins, non-structural proteins. replicases, antisense and satellite RNAs for the production of virus-resistant transgenic plants are briefly discussed. The concept of recombination of viral RNA with transgene products is critically analyzed and potential risks associated with commercialization of transgenic plants with viral inserts and its effects on bio-safety, are highlighted.

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Section: Pathogen-derived Resistance Strategies In Crop Protectionsupporting

confidence: 76%

Pathogen-Derived Resistance Against Plant Viruses: Postscript and Prospects

Arif¹,

Hassan²

1999

Pakistan J. of Biological Sciences

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“…Higher concentrations of virus inoculum or (partially) unencapsidated viral RNA overcame CPMP. However, these simple rules derived from the first model systems [TMV, tobacco rattle virus, tobacco streak virus, or AIMV in tobacco (4,(44)(45)(46)] were quickly broken by cucumber mosaic virus (CMV) and potato virus X (PVX), where antisense CP mRNA provided protection (47,48), and for PVX and potato virus S [a carlavirus (49)], where resistance to naked RNA inocula occurred.…”

Section: On Cp-mediated Protection and Proposed Mechanismsmentioning

confidence: 99%

Strategies to protect crop plants against viruses: pathogen-derived resistance blossoms.

Wilson¹

1993

Proc. Natl. Acad. Sci. U.S.A.

229

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Since 1986, the ability to confer resistance against an otherwise devastating virus by introducing a single pathogen-derived or virus-targeted sequence into the DNA of a potential host plant has had a marked influence on much of the research effort, focus, and shortterm objectives of plant virologists throughout the world. The vast literature on coat protein-mediated protection, for example, attests to our fascination for unraveling fundamental molecular mechanism(s), our (vain) Despite extensive and sometimes elegant experimentation, the molecular mechanism(s) of this viral "crossprotection" have remained elusive and controversial. In some cases, the coat protein (CP) of the protectant virus was thought to be primarily responsible, either by preventing particle disassembly or by re-encapsidating the incoming genome of the more severe challenge virus. However, viroids [240-to 380-nt-long, naked circular single-stranded (ss)RNA pathogens] and mutant viruses making assembly-defective or no detectable CP could also cross-protect against their more severe relatives. Such observations prompted models based on inhibitory interactions between sense and antisense RNAs or between the replicational machineries ofthe two competing pathogens (6). Controversy arose largely because available molecular technology could not resolve which regulatory or coding sequence(s) or polypeptide product(s) from the actively replicating genome of the primary (protectant) pathogen were responsible for interfering with the many replicative processes essential to establish infection by the secondary, related, and more severe virus. In contrast, multiple infections by unrelated viruses sharing a common host are very prevalent in nature. Many aspects of this older story have their parallels in current hypotheses and lack of a unified model for pathogenderived resistance in transgenic plants.The advent of improved cell and tissue culture techniques, efficient protocols for Agrobacterium tumefaciens-mediated transformation and plantlet regeneration in dicotyledonous species (7) [and more recent methods for monocot crops (8-10)], has permitted, among other applications (11,12), the theory of pathogenderived virus resistance to be tested in practice.Collaboration between researchers at Monsanto and Washington University (St. Louis) led to the first report of CPmediated protection (CPMP) against tobacco mosaic virus (TMV) in tobacco in 1986 (4). Since then, CPMP has been reported for over 20 viruses in at least 10 different taxonomic groups, in a wide 3134 variety of dicot plant species, and the list is increasing rapidly.

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“…sidered as a way to develop PRSV resistant cucurbits and papaya varieties. Coat protein mediated resistance (Abel et al, 1986) has been successfully applied to a wide range of host-virus combinations (Nelson et al, 1988;Kaniewski et al, 1990;MacKenzie & Tremaine, 1990;Quemada et al, 1991;Brault et al, 1993) including several potyviruses (Da Camara Machado et al, 1992;Stark & Beachy, 1989;Namba et al, 1992;Ling et al, 1991) including PRSV in papaya (Fitch et al, 1992). However, this resistance can be very strain or isolate specific, probably depending on the relatedness of the transgene and the challenge virus (Sanders et al, 1992;Nelson et al, 1988;Quemada et al, 1991;Nakajima et al, 1993).…”

Section: Introductionmentioning

confidence: 99%

Papaya ringspot potyvirus: isolate variability and the origin of PRSV type P (Australia)

Bateson

Chaleeprom

et al. 1994

Journal of General Virology

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We have sequenced the coat protein gene of nine isolates of papaya ringspot virus (PRSV) including six Australian and three Asian isolates and compared these with four previously reported sequences of PRSV. There was up to 12 % sequence variation between isolates at the nucleotide level. However, there was no significant difference between the sequences obtained from Australian isolates irrespective of whether they were PRSV type P (cucurbit or papaya infecting) or PRSV type W (cucurbit infecting) and these isolates were more closely related to one another than to any other isolate. These results imply that PRSV-P, first recorded in Australia in 1991, arose locally from PRSV-W (first recorded in Australia in 1978) rather than being introduced. Further, there was no consistent sequence difference between PRSV-P and PRSV-W isolates that would obviously account for their host range difference.

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Transgenic Nicotiana debneyii expressing viral coat protein are resistant to potato virus S infection

Cited by 40 publications

References 30 publications

Pathogen-Derived Resistance Against Plant Viruses: Postscript and Prospects

Pathogen-Derived Resistance Against Plant Viruses: Postscript and Prospects

Strategies to protect crop plants against viruses: pathogen-derived resistance blossoms.

Papaya ringspot potyvirus: isolate variability and the origin of PRSV type P (Australia)

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