Transgenic mouse models generated by hydrodynamic transfection for genetic studies of liver cancer and preclinical testing of anti‐cancer therapy

Ju, Hye Lim; Han, Kwang Hyub; Lee, Jong Doo; Ro, Simon Weonsang

doi:10.1002/ijc.29703

Cited by 28 publications

(18 citation statements)

References 104 publications

(176 reference statements)

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“…However, it is very expensive and time-consuming to generate a GEM model. Recently, HGD was applied to generate many models of HCC by over-expressing candidate oncogenes, coupled with the sleeping beauty transposon/transposase system (Ju et al, 2016 ). The sleeping beauty transposon /transposase vectors induced stable chromosomal integration and persistent transgene expression.…”

Section: Establishment Of Hepatocellular Carcinoma Models By Hgdmentioning

confidence: 99%

Technical Improvement and Application of Hydrodynamic Gene Delivery in Study of Liver Diseases

et al. 2017

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Development of an safe and efficient in vivo gene delivery method is indispensable for molecular biology research and the progress in the following gene therapy. Over the past few years, hydrodynamic gene delivery (HGD) with naked DNA has drawn increasing interest in both research and potential clinic applications due to its high efficiency and low risk in triggering immune responses and carcinogenesis in comparison to viral vectors. This method, involving intravenous injection (i.v.) of massive DNA in a short duration, gives a transient but high in vivo gene expression especially in the liver of small animals. In addition to DNA, it has also been shown to deliver other substance such as RNA, proteins, synthetic small compounds and even viruses in vivo. Given its ability to robustly mimic in vivo hepatitis B virus (HBV) production in liver, HGD has become a fundamental and important technology on HBV studies in our group and many other groups. Recently, there have been interesting reports about the applications and further improvement of this technology in other liver research. Here, we review the principle, safety, current application and development of hydrodynamic delivery in liver disease studies, and discuss its future prospects, clinical potential and challenges.

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Section: Establishment Of Hepatocellular Carcinoma Models By Hgdmentioning

confidence: 99%

Technical Improvement and Application of Hydrodynamic Gene Delivery in Study of Liver Diseases

et al. 2017

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“…To investigate hepatic carcinogenesis induced by the MAPK/ERK signaling pathway, many researchers have used a transgenic approach. Various transgenic mouse models have been developed using this method, which show high similarity to the pathological and molecular properties of human HCC [ 82 , 83 ]. A selection of genetically engineered mouse (GEM) models is summarized in Table 2 , with emphasis on the activation of MAPK/ERK signaling.…”

Section: Mapk/erk Signaling Pathway In Hccmentioning

confidence: 99%

MAPK/ERK Signaling Pathway in Hepatocellular Carcinoma

Moon

2021

Cancers

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126

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Hepatocellular carcinoma (HCC) is a major health concern worldwide, and its incidence is increasing steadily. Recently, the MAPK/ERK signaling pathway in HCC has gained renewed attention from basic and clinical researchers. The MAPK/ERK signaling pathway is activated in more than 50% of human HCC cases; however, activating mutations in RAS and RAF genes are rarely found in HCC, which are major genetic events leading to the activation of the MAPK/ERK signaling pathway in other cancers. This suggests that there is an alternative mechanism behind the activation of the signaling pathway in HCC. Here, we will review recent advances in understanding the cellular and molecular mechanisms involved in the activation of the MAPK/ERK signaling pathway and discuss potential therapeutic strategies targeting the signaling pathway in the context of HCC.

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“…However, so far these models have been used to study the effects of gene changes, e.g. , mutations, deletions, or overexpression, during the onset of hepatoma, rather than at the point of hepatocarcinogenesis 89. A significant breakthrough in the application of mouse models to study hepatoma in this area is the development of ribonucleic acid (RNA) microarray technology.…”

Section: Rodent Models Of Hepatomamentioning

confidence: 99%

Evaluation of Polymer Nanoformulations in Hepatoma Therapy by Established Rodent Models

Wang

Zhang

et al. 2019

Theranostics

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Hepatoma is one of the most severe malignancies usually with poor prognosis, and many patients are insensitive to the existing therapeutic agents, including the drugs for chemotherapy and molecular targeted therapy. Currently, researchers are committed to developing the advanced formulations with controlled drug delivery to improve the efficacy of hepatoma therapy. Numerous inoculated, induced, and genetically engineered hepatoma rodent models are now available for formulation screening. However, animal models of hepatoma cannot accurately represent human hepatoma in terms of histological characteristics, metastatic pathways, and post-treatment responses. Therefore, advanced animal hepatoma models with comparable pathogenesis and pathological features are in urgent need in the further studies. Moreover, the development of nanomedicines has renewed hope for chemotherapy and molecular targeted therapy of advanced hepatoma. As one kind of advanced formulations, the polymer-based nanoformulated drugs have many advantages over the traditional ones, such as improved tumor selectivity and treatment efficacy, and reduced systemic side effects. In this article, the construction of rodent hepatoma model and much information about the current development of polymer nanomedicines were reviewed in order to provide a basis for the development of advanced formulations with clinical therapeutic potential for hepatoma.

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Transgenic mouse models generated by hydrodynamic transfection for genetic studies of liver cancer and preclinical testing of anti‐cancer therapy

Cited by 28 publications

References 104 publications

Technical Improvement and Application of Hydrodynamic Gene Delivery in Study of Liver Diseases

Technical Improvement and Application of Hydrodynamic Gene Delivery in Study of Liver Diseases

MAPK/ERK Signaling Pathway in Hepatocellular Carcinoma

Evaluation of Polymer Nanoformulations in Hepatoma Therapy by Established Rodent Models

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