Transgenic mice expressing surface markers for IFN-γ and IL-4 producing cells

A Distal Conserved Sequence Element Controls Ifng Gene Expression by T Cells and NK Cells

“…Similarly, comparable frequencies of Th2 cells derived from transgenic and nontransgenic mice expressed IL-4, demonstrating that the transgene had no untoward effects on Th1 or Th2 development. As anticipated from prior reports of allelic utilization of cytokine genes (Hsieh et al, 2000;Kelly and Locksley, 2000), or perhaps because of position effect variegation of the transgenic allele, the fraction of IFNg+ T cells that coexpressed Thy1.1 was approximately one-third of the total of IFNg+ Th1 cells in hemizygous transgenic mice (14.1% Thy1.1+/IFNg+ versus 50.0% total IFNg+, Figure 5, and see Figure 6). Importantly, however, essentially all of the cells that expressed the Thy1.1 reporter also expressed IFNg, validating the model for accurate identification of IFNg+ CD4 + T cells.…”

Immunopathogenic Mechanism and Therapeutic Intervention in an Experimental Murine Model of Membranous Nephropathy

“…T1/T2 TG mice still has advantages of stable and stronger signal expression, a wider time window for detection, lack of requirement of restimulation or permeabilization, and possible isolation of viable cells of given Th phenotypes. Thus, these T1/T2 TG mice are useful in providing a feasible and direct in vivo monitoring system for dissecting the changes in proportions of pathogenic T cells during the pathogenic or therapeutic processes using flow cytometry with surface immunofluorescent staining (Hsieh et al, 2000;Hung et al, 2005;Sung et al, 2004). A progressive increase in Th2 cells were observed in splenocytes as well as in peripheral blood and kidney cells.…”

“…However, Penny et al found that the progressive development of infiltrates of activated T cells-principally Th1 and cytotoxic effector cells-as well as macrophages identified within glomeruli of Lews rats with HN, coincides with the development of proteinuria (Penny et al, 1998). Early studies suggested that classical T cell effector responses are involved in HN, including passive transfer of HN to tolerant rats by lymphoid cells but not serum, and lymphoid cells in culture (Heymann et al, 1962;Hsieh et al, 2000). Furthermore, it has also been found that permanent CD8 + T cell depletion both early and late in the course of disease prevents proteinuria in active Heymann Nephritis.…”

“…To directly investigate immunoregulatory process and immunopathological mechanisms in cBSA-induced MN, we used TH1/TH2 double transgenic mouse (T1/T2 TG mice) which provides the best in vivo model to study the differentiation of helper T cell subsets during the Th process was used (Wu et al, 2007). T1/T2 TG mice, originally in BALB/c background, bear two transgenes expressing two distinct cell surface markers, one is human Thy1 protein (human CD90) under the murine IFNγ promoter control, the other is murine Thy1.1 protein (murine CD90.1) under the murine IL-4 promoter control, designated as TH1/TH2 transgenes respectively (Hsieh et al, 2000;Hung et al, 2005). T1/T2 TG MN mice showed overt proteinuria, hypoalbuminemia, and hypercholesterolemia characteristically.…”

Kinetics of adaptive immunity to Haemophilus influenzae type b meningitis in transgenic mice: Evidence from diverse expression of double T1/T2 transgenes and Th1/Th2-related cytokines

“…These mice carry two transgenes that express two distinct cell-surface markers: a human Thy1 transgene (hThy1) under the control of the murine IFN-γ promoter, and a murine Thy1.1 transgene (mThy1.1) under the control of the murine IL-4 promoter, designated T1 and T2, respectively. These transgenic mice have been used previously as a model to monitor the in vivo development of Th1 or Th2 cells during Listeria monocytogenes or Schistosoma mansoni infections [14]. These T1/T2 doubly transgenic mice have currently been used as a monitor system in autoimmune diabetes [15,16].…”