Transgenic lines of melon (Cucumis melo L. var. makuwa cv. ‘Silver Light’) expressing antifungal protein and chitinase genes exhibit enhanced resistance to fungal pathogens

Bezirganoğlu, İsmail; Hwang, Shih Ying; Fang, Tony J.; Shaw, Jei‐Fu

doi:10.1007/s11240-012-0227-5

Cited by 42 publications

(25 citation statements)

References 39 publications

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“…Antifungal protein (AFP) and chitinase (CHI) fusion (AFP-CHI) gene protects transgenic oriental melon plants against diseases caused by the fungi R. solani and F. oxysporum (Bezirganoglu et al 2013 Overexpression of full-length genes encoding two Petunia flower defensins, PhDef1 and PhDef2, in transgenic banana plants was achieved by employing embryogenic cells as explants for Agrobacterium-mediated genetic transformation. High-level constitutive expression of these defensins in banana plants reinforced resistance against F. oxysporum infection in vitro as well as ex vivo (Ghag et al 2012).…”

Section: Application In Other Plantsmentioning

confidence: 99%

Plant antifungal proteins and their applications in agriculture

Yan

Yuan

Jiang

et al. 2015

Appl Microbiol Biotechnol

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Fungi are far more complex organisms than viruses or bacteria and can develop numerous diseases in plants that cause loss of a substantial portion of the crop every year. Plants have developed various mechanisms to defend themselves against these fungi which include the production of low-molecular-weight secondary metabolites and proteins and peptides with antifungal activity. In this review, families of plant antifungal proteins (AFPs) including defensins, lectins, and several others will be summarized. Moreover, the application of AFPs in agriculture will also be analyzed.

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Section: Application In Other Plantsmentioning

confidence: 99%

Plant antifungal proteins and their applications in agriculture

Yan

Yuan

Jiang

et al. 2015

Appl Microbiol Biotechnol

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“…Nilai bobot buah yang lebih kecil ini tidak selalu berarti sebuah kekurangan, meskipun pada umumnya petani maupun konsumen mengharapkan ukuran dan bobot buah melon yang besar (Wagiono dan Hamrah, 2007). Varietas melon berukuran kecil sudah dipasarkan di negara lain seperti di Taiwan yang ditujukan untuk konsumsi pribadi dan dapat dihabiskan dalam satu atau dua kesempatan (Bezirganoglu et al, 2013). Daya hasil per hektar dari melon tipe kecil diharapkan dapat diimbangi dengan produksi dua sampai tiga buah per tanaman, dimana penelitian lebih lanjut diperlukan untuk hal ini.…”

Section: Evaluasi Terhadap Karakter Kuantitatif Tanamanunclassified

Evaluasi Karakteristik Hortikultura Empat Genotipe Melon (Cucumis melo L.) Pusat Kajian Hortikultura Tropika IPB

Khumaero¹,

Efendi²,

Suwarno³

et al. 2015

J Hort Indonesia

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Center for Tropical Horticulture Studies (CTHS) have conducted melon breeding to meet the need of the expanding melon production in ABSTRAKPusat Kajian Hortikultura Tropika (PKHT) telah melakukan penelitian pemuliaan tanaman untuk mengetahui kebutuhan pengembangan produksi melon di Indonesia. Empat genotipe melon yakni IPB Meta 3, IPB Meta 4, IPB Meta 6, IPB Meta 8H menunjukkan penampilan baik pada percobaan sebelumnya. Sebelum dilepas atau didaftarkan, diperlukan evaluasi karakteristik utama dari empat genotipe tersebut. Pengujian keempat genotipe potensial dengan dua varietas pembanding yakni Action 434 dan Sky Sweet disusun berdasarkan Rancangan Kelompok Lengkap Teracak (RKLT) dengan 4 ulangan. Hasilnya menunjukkan bahwa genotipe melon IPB Meta 4 memiliki diameter batang dan ukuran daun yang lebih besar dibandingkan Action 434 dan Sky Sweet. Selain itu, daging buah melon genotipe IPB Meta 3, IPB Meta 6, dan IPB Meta 8H berwarna jingga, dimana kedua varietas pembanding berwarna hijau. Hasil ini menunjukkan bahwa genotipe-genotipe melon yang dikembangkan di PKHT memiliki karakteristik yang unik, dimana dapat berpotensi bagi segmen pasar khusus.Kata kunci: karakteristik morfologi, kualitas buah, melon PENDAHULUAN Melon (Cucumis melo L.) merupakan salah satu komoditas buah-buahan yang banyak digemari oleh masyarakat karena melon memiliki berbagai keunggulan berupa rasa yang manis dan warna daging buah yang bervariasi. Selain itu melon memiliki nilai ekonomi dan prospek yang menjanjikan dalam aspek pemasaran (Sudiyarto, 2011).Agribisnis melon memiliki peluang besar karena harganya yang cukup tinggi (Prajnanta, 2004). Melon dikenal juga sebagai buah yang menyehatkan karena mengandung berbagai vitamin dan mineral yang diperlukan tubuh

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“…Chitinases are capable of degrading fungal cell walls and are therefore thought to play a major role in the plant's response (Collinge et al 1993). Antifungal proteins (AFPs) belong to a group of plant defense proteins, present in different plant tissues ranging from leaves, stems, and roots to seeds (Bezirganoglu et al 2013). The production of transgenic plants' overexpression of antifungal proteins (AFP) and chitinase gene (CHI) may confer protection to pathogen attack, and this protection is increased due to the synergic effect of the expression of two or more genes (Fiocchetti et al 2008).…”

Section: Introductionmentioning

confidence: 99%

“…Development of disease resistance by transfer of resistance genes into crops is valuable in terms of cost and time saving. In recent years, traditional melon breeding in combination with transgenic technology has been applied to address melon fungal disease, which lead to production of some disease tolerant melon plants (Bezirganoglu et al 2013). Using Agrobacterium tumefaciens method, AFPCHI gene was transferred to the Cucumis melo L. Silver light and a disease resistant transgenic melon line named D1 was developed.…”

Section: Introductionmentioning

confidence: 99%

Impact of transgenic AFPCHI (Cucumis melo L. Silver Light) fungal resistance melon on soil microbial communities and enzyme activities

Bezirganoğlu

Uysal

2017

J Plant Biotechnol

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This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.Abstract A greenhouse experiment was conducted for evaluation of ecological effects of transgenic melon plants in the rhizospheric soil in terms of soil properties, enzyme activities and microbial communities. Organic matter content of soil under transgenic melon plants was significantly higher than that of soil with non-transgenic melon plants. Significant variations were observed in organic matter, total P and K in soil cultivation with transgenic melon plants. There were also significant variations in the total numbers of colony forming units of fungi, actinomycetes and bacteria between soils treated with transgenic and non-transgenic melon plants. Transgenic and non-transgenic melon significantly enhanced several enzymes activities including urease, acid phosphatase, alkalin phosphatase, arysulphtase, β glucosidase, dehydrogenase, protease and catalase. Soil polyphenoloxidase activity of T 1 transgenic melon was lower than that of T 0 transgenic melon and a non-melon plant during the same period. The first generation transgenic melon plants (T 0 ) showed significantly greater (p<0.05) effect on the activitiy of arylsulfatase, which increased from 2.540x10 6 CFU g -1 (control) to 19.860x10 6 CFU g -1 (T 0 ). These results clearly indicated that transgenic melon might change microbial communities, enzyme activities and soil chemical properties.

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Transgenic lines of melon (Cucumis melo L. var. makuwa cv. ‘Silver Light’) expressing antifungal protein and chitinase genes exhibit enhanced resistance to fungal pathogens

Cited by 42 publications

References 39 publications

Plant antifungal proteins and their applications in agriculture

Plant antifungal proteins and their applications in agriculture

Evaluasi Karakteristik Hortikultura Empat Genotipe Melon (Cucumis melo L.) Pusat Kajian Hortikultura Tropika IPB

Impact of transgenic AFPCHI (Cucumis melo L. Silver Light) fungal resistance melon on soil microbial communities and enzyme activities

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