Transgenic insect resistance traits increase corn yield and yield stability

Edgerton, Michael D.; Fridgen, J. J.; Anderson, John R.; Ahlgrim, Jenne; Criswell, Monty; Dhungana, Prabhakar; Gocken, Tom; Li, Zheng; Mariappan, Sadayappan; Pilcher, Clinton D.; Rosielle, A. A.; Stark, Steven B.

doi:10.1038/nbt.2259

Cited by 92 publications

(56 citation statements)

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“…Biotech crops engineered to express proteins from the bacterium Bacillus thuringiensis (Bt) have become a major tool for managing corn, cotton and soybean insect pests worldwide . Environmental and economic benefits associated with the adoption of Bt crops have been well recognized in numerous studies . However, evolution of resistance to Bt proteins in insects is a threat to the sustainable use of Bt crop technology .…”

Section: Introductionmentioning

confidence: 99%

F₂ screen, inheritance and cross‐resistance of field‐derived Vip3A resistance in Spodoptera frugiperda (Lepidoptera: Noctuidae) collected from Louisiana, USA

Yang

Morsello

Head

et al. 2018

Pest Management Science

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Section: Introductionmentioning

confidence: 99%

F₂ screen, inheritance and cross‐resistance of field‐derived Vip3A resistance in Spodoptera frugiperda (Lepidoptera: Noctuidae) collected from Louisiana, USA

Yang

Morsello

Head

et al. 2018

Pest Management Science

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“…The area planted to Bt crops worldwide increased to 76 million hectares (ha) in 2013, with a cumulative total of >560 million ha since 19964. Although Bt crops have provided substantial environmental and economic benefits5678910 evolution of resistance to Bt proteins by pests can cut short these benefits11121314.…”

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confidence: 99%

Mis-splicing of the ABCC2 gene linked with Bt toxin resistance in Helicoverpa armigera

Xiao

Zhang

Liu

et al. 2014

Sci Rep

151

147

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Toxins from the bacterium Bacillus thuringiensis (Bt) are used widely for insect control in sprays and transgenic plants, but their efficacy is reduced when pests evolve resistance. Previous work showed that mutations in a gene encoding the transporter protein ABCC2 are linked with resistance to Bt toxins Cry1Ab, Cry1Ac or both in four species of Lepidoptera. Here we compared the ABCC2 gene of Helicoverpa armigera (HaABCC2) between susceptible strains and a laboratory-selected strain with >1,000-fold resistance to Cry1Ac relative its susceptible parent strain. We discovered a 73-base pair (bp) insertion in the cDNA of the resistant strain that generates a premature stop codon expected to yield a truncated ABCC2 protein. Sequencing of genomic DNA revealed that this insertion is an intron that is not spliced out because of a 6-bp deletion at its splicing site. Analysis of progeny from crosses revealed tight genetic linkage between HaABCC2 and resistance to Cry1Ac. These results provide the first evidence that mis-splicing of a gene encoding an ABCC2 protein confers resistance to a Bt toxin.

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“…In 2013, 90% of all corn acres in the United States were planted with some type of transgenic corn hybrid (USDA-NASS, 2013). Transgenic insect resistance traits have been demonstrated to increase corn yields by an average of 0.5 Mg ha -1 compared to non-traited isolines, a value which increases under conditions of stress (Edgerton et al, 2012). There are two general types of transgenic insect resistance traits available in corn hybrids; both are obtained from soilborne bacteria called Bacillius thuringiensis and are referred to as "Bt" genes (Nielsen, 2010).…”

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confidence: 99%

Evaluating Management Factor Contributions to Reduce Corn Yield Gaps

Ruffo¹,

Gentry

Henninger

et al. 2015

Agronomy Journal

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ABSTRACTe need to intensify agricultural production due to a growing human population requires yield gaps to be closed. In 2009 and 2010, ve management factors were assessed for their individual and cumulative contributions to reducing the corn (Zea mays L.) yield gap and yield components in a corn-soybean [Glycine max (L.) Merr.] rotation. Five management factors (plant population, transgenic insect resistance, fungicide containing strobilurin, P-S-Zn fertility, and N fertility) were evaluated. An incomplete factorial design with these factors resulted in 12 treatments, including two controls: high technology (HT) and standard technology (ST), comprising all ve factors applied at the supplemental or the standard level, respectively. e HT control yielded 2.9 Mg ha -1 (2.12-3.50 Mg ha -1 across sites and years) more grain (28%) than the ST control, demonstrating the yield gap between traditional farm practice and attainable yield using available technologies. All management factors except plant population were necessary for reducing the yield gap. Fungicide and Bacillius thuringiensis gene (Bt) traits provided the greatest yield increases compared to the ST system. Averaged over sites and years, if each factor was withheld from the HT system, yield decreased by decreasing kernel number. Increased plant population reduced the yield gap when all other inputs were applied at the supplemental level. Kernel number was more signi cant for increasing yield than kernel weight. e yield contribution of each factor was greater when applied as part of a full complement of supplemental inputs than when added individually to the standard input system. Abbreviations: Bt, Bacillius thuringiensis gene; -Bt, HT control system with non-Bt variety; +Bt, ST control with Bt-containing variety added; CU, Champaign-Urbana study site; DS, Dixon Springs Research Center site; -Fung, HT control treatment without fungicide; +Fung, ST control treatment with added fungicide; HT, high technology level; +N, additional side-dress N to ST control; -N, HT control without side-dress application; +Pop, high population treatment added to ST control; -Pop, decreased population treatment within HT control; +P-S-Zn, P, S, and Zn combination fertilizer added to ST control; -P-S-Zn, HT control treatment without P-S-Zn combination fertilizer; ST, standard technology level; VT, tasseling.Numerous recent papers have established that agricultural production must increase substantially to meet the increasing per capita demand for food, feed, fuel, and fi ber of a burgeoning human population (Keyzer et al., 2005, Food and Agriculture Organization, 2009. From a global perspective, it is generally agreed that agricultural intensifi cation (increasing agricultural production per unit area) is preferable to extensifi cation (expanding agriculture onto new areas) as a means of increasing crop production Burney et al., 2010;McLaughlin, 2011;Tilman et al., 2011;Foley et al., 2011). In contrast to the question of whether yield increases are needed, the more practical i...

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Transgenic insect resistance traits increase corn yield and yield stability

Cited by 92 publications

References 28 publications

F₂ screen, inheritance and cross‐resistance of field‐derived Vip3A resistance in Spodoptera frugiperda (Lepidoptera: Noctuidae) collected from Louisiana, USA

F₂ screen, inheritance and cross‐resistance of field‐derived Vip3A resistance in Spodoptera frugiperda (Lepidoptera: Noctuidae) collected from Louisiana, USA

Mis-splicing of the ABCC2 gene linked with Bt toxin resistance in Helicoverpa armigera

Evaluating Management Factor Contributions to Reduce Corn Yield Gaps

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Transgenic insect resistance traits increase corn yield and yield stability

Cited by 92 publications

References 28 publications

F2 screen, inheritance and cross‐resistance of field‐derived Vip3A resistance in Spodoptera frugiperda (Lepidoptera: Noctuidae) collected from Louisiana, USA

F2 screen, inheritance and cross‐resistance of field‐derived Vip3A resistance in Spodoptera frugiperda (Lepidoptera: Noctuidae) collected from Louisiana, USA

Mis-splicing of the ABCC2 gene linked with Bt toxin resistance in Helicoverpa armigera

Evaluating Management Factor Contributions to Reduce Corn Yield Gaps

Contact Info

Product

Resources

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F₂ screen, inheritance and cross‐resistance of field‐derived Vip3A resistance in Spodoptera frugiperda (Lepidoptera: Noctuidae) collected from Louisiana, USA

F₂ screen, inheritance and cross‐resistance of field‐derived Vip3A resistance in Spodoptera frugiperda (Lepidoptera: Noctuidae) collected from Louisiana, USA