Transgenic expression of Hyp-1 gene from Hypericum perforatum L. alters expression of defense-related genes and modulates recalcitrance to Agrobacterium tumefaciens

Hou, Weina; Singh, Rupesh Kumar; Zhao, Pan; Martins, Viviana; Aguilar, Emmanuel; Canto, Tomás; Tenllado, Francisco; Dias, Alberto Carlos Pires

doi:10.1007/s00425-019-03310-3

Cited by 5 publications

(20 citation statements)

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“…An analog has also been isolated in lupine, and was found to form a complex with both melatonin and the cytokinin zeatin (Sliwiak et al, 2018a,b). Overexpression of Hyp-1 in both tobacco (N. tabacum) and lettuce (Lactuca sativa) found that Hyp-1 localized to the nucleus, plasma membrane and cytoplasm of epidermal cells and was found to confer resistance to bacterial infection with Agrobacterium tumefaciens (Hou et al, 2019). Together these results suggest that Hyp-1 may be a mediator of melatonin-phytohormone cross talk to control responses to biotic challenge.…”

Section: Major Research Theme -Phytohormone and Plant Signalingmentioning

confidence: 92%

“…In some cases, pathogens have also been found to be capable of inhibition of host melatonin production, potentially enhancing melatonin susceptibility to infection, and indicating an important role for melatonin in pathogen response (Nehela and Killiny, 2018). This phenomenon has been investigated as an approach for enhancing efficiency of Agrobacterium-mediated transformation in recalcitrant species (Dan et al, 2015;Hou et al, 2019).…”

Section: Major Theme -Interkingdom Communicationmentioning

confidence: 99%

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A Systematic Review of Melatonin in Plants: An Example of Evolution of Literature

Murch

Erland

2021

Front. Plant Sci.

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Melatonin (N-acetyl-5-methoxy-tryptamine) is a mammalian neurohormone, antioxidant and signaling molecule that was first discovered in plants in 1995. The first studies investigated plant melatonin from a human perspective quantifying melatonin in foods and medicinal plants and questioning whether its presence could explain the activity of some plants as medicines. Starting with these first handful of studies in the late 1990s, plant melatonin research has blossomed into a vibrant and active area of investigation and melatonin has been found to play critical roles in mediating plant responses and development at every stage of the plant life cycle from pollen and embryo development through seed germination, vegetative growth and stress response. Here we have utilized a systematic approach in accordance with the preferred reporting items for systematic reviews and meta-analyses (PRISMA) protocols to reduce bias in our assessment of the literature and provide an overview of the current state of melatonin research in plants, covering 1995–2021. This review provides an overview of the biosynthesis and metabolism of melatonin as well as identifying key themes including: abiotic stress responses, root development, light responses, interkingdom communication, phytohormone and plant signaling. Additionally, potential biases in the literature are investigated and a birefringence in the literature between researchers from plant and medical based which has helped to shape the current state of melatonin research. Several exciting new opportunities for future areas of melatonin research are also identified including investigation of non-crop and non-medicinal species as well as characterization of melatonin signaling networks in plants.

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Section: Major Research Theme -Phytohormone and Plant Signalingmentioning

confidence: 92%

Section: Major Theme -Interkingdom Communicationmentioning

confidence: 99%

A Systematic Review of Melatonin in Plants: An Example of Evolution of Literature

Murch

Erland

2021

Front. Plant Sci.

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“…Recently, a PR 10 gene from the Agrobacterium- recalcitrant plant Hypericum perforatum has been identified and named as phenolic oxidative coupling protein (Hyp-1). This defence gene has been found to hinder Agrobacterium infection in Tobacco (Hou et al 2020 ). During infection, Hyp-1 transcripts get upregulated and play an important role in plant defence (Karppinen et al 2016 ).…”

Section: Defence Responsive Genesmentioning

confidence: 99%

“…It has been suggested that Hyp-1 confers tolerance to Agrobacterium by downregulating auxin signalling pathway genes, such as NtaTIR1 and NtaAFR8 . During pathogen attack, Hyp-1 induces the expression of MiR160, which targets the ARF10 , ARF16 and ARF17 transcripts of the auxin signalling cascade and downregulates their expression (Hou et al 2020 ; Pinweha et al 2015 ; Wójcik et al 2017 ). Dunoyer found low levels of small RNAs associated with the iaaM (tryptophan 2 oxygenase) and ags (agropine synthase) genes of T-DNA in Nicotiana tabaccum after 3 days of Agrobacterium infiltration.…”

Section: Defence Responsive Genesmentioning

confidence: 99%

Agrobacterium-mediated gene transfer: recent advancements and layered immunity in plants

Tiwari

Mishra

Chakrabarty

2022

Planta

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Main conclusion Plant responds to Agrobacterium via three-layered immunity that determines its susceptibility or resistance to Agrobacterium infection. Abstract Agrobacterium tumefaciens is a soil-borne Gram-negative bacterium that causes crown gall disease in plants. The remarkable feat of interkingdom gene transfer has been extensively utilised in plant biotechnology to transform plant as well as non-host systems. In the past two decades, the molecular mode of the pathogenesis of A. tumefaciens has been extensively studied. Agrobacterium has also been utilised as a premier model to understand the defence response of plants during plant– Agrobacterium interaction. Nonetheless, the threat of Agrobacterium -mediated crown gall disease persists and is associated with a huge loss of plant vigour in agriculture. Understanding the molecular dialogues between these two interkingdom species might provide a cure for crown gall disease. Plants respond to A. tumefaciens by mounting a three-layered immune response, which is manipulated by Agrobacterium via its virulence effector proteins. Comparative studies on plant defence proteins versus the counter-defence of Agrobacterium have shed light on plant susceptibility and tolerance. It is possible to manipulate a plant’s immune system to overcome the crown gall disease and increase its competence via A. tumefaciens -mediated transformation. This review summarises the recent advances in the molecular mode of Agrobacterium pathogenesis as well as the three-layered immune response of plants against Agrobacterium infection.

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“…In our previous studies, two defense genes, Phenolic oxidative coupling protein (Hyp-1) and Polygalacturonase-inhibiting protein (PGIP) were selected from a forward subtractive cDNA library from H. perforatum cell suspension cultures elicited with A. tumefaciens. Overexpression of Hyp-1, a pathogenesis-related (PR) class 10 family gene, in tobacco and lettuce greatly decreased Agrobacterium-mediated expression efficiency in transgenic plants (Hou et al 2020a).…”

Section: Introductionmentioning

confidence: 99%

Transcriptional responses of

Hou

Singh²,

Martins

et al. 2021

Funct. Plant Biol.

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Hypericum perforatum L., known as St. John's wort, is a medicinal plant with anti-depressant, anti-inflammatory, and anti-cancer properties. H. perforatum is recalcitrant to transformation by Agrobacterium tumefaciens, which limits metabolic engineering studies. We explored the expression profiles of key genes involved in several plant defense responses to get insights into the molecular basis of this recalcitrance. Our results suggest that secondary metabolites could impair pathogen´s growth, making H. perforatum recalcitrant to A. tumefaciens-mediated transformation.

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Transgenic expression of Hyp-1 gene from Hypericum perforatum L. alters expression of defense-related genes and modulates recalcitrance to Agrobacterium tumefaciens

Abstract: Phenolic oxidative coupling protein (Hyp-1) isolated from Hypericum perforatum L. was characterized as a defense gene involved into H. perforatum L. recalcitrance to Agrobacterium tumefaciens-mediated transformation.

Cited by 5 publications

References 68 publications

A Systematic Review of Melatonin in Plants: An Example of Evolution of Literature

A Systematic Review of Melatonin in Plants: An Example of Evolution of Literature

Agrobacterium-mediated gene transfer: recent advancements and layered immunity in plants

Transcriptional responses of

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