Transgenic expression of a gene encoding a synthetic antimicrobial peptide results in inhibition of fungal growth in vitro and in planta

Cary, Jeffrey W.; Rajasekaran, Kanniah; Jaynes, Jesse M.

doi:10.1016/s0168-9452(00)00189-8

Cited by 111 publications

(70 citation statements)

References 32 publications

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“…Small size facilitates prospects for both large-scale synthetic production and natural expression in a genetically modified mosquito. A precedence for the latter has already been demonstrated with a genetically modified tobacco plant, which expressed a synthetic antimicrobial peptide active against pathogenic fungi (10).…”

Section: Discussionmentioning

confidence: 99%

Design and Activity of Antimicrobial Peptides against Sporogonic-Stage Parasites Causing Murine Malarias

Arrighi

Nakamura

Miyake

et al. 2002

Antimicrob Agents Chemother

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Insects produce several types of peptides to combat a broad spectrum of invasive pathogenic microbes, including protozoans. However, despite this defense response, infections are often established. Our aim was to design novel peptides that produce high rates of mortality among protozoa of the genus Plasmodium, the malaria parasites. Using existing antimicrobial peptide sequences as templates, we designed and synthesized three short novel hybrids, designated Vida1 to Vida3. Each has a slightly different predicted secondary structure. The peptides were tested against sporogonic stages of the rodent malaria parasites Plasmodium berghei (in vitro and in vivo) and P. yoelii nigeriensis (in vitro). The level of activity varied for each peptide and according to the parasite stage targeted. Vida3 (which is predicted to have large numbers of ␤ sheets and coils but no ␣ helices) showed the highest level of activity, killing the early sporogonic stages in culture and causing highly significant reductions in the prevalence and intensity of infection of P. berghei after oral administration or injection in Anopheles gambiae mosquitoes. The secondary structures of these peptides may play a crucial role in their ability to interact with and kill sporogonic forms of the malaria parasite.

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Section: Discussionmentioning

confidence: 99%

Design and Activity of Antimicrobial Peptides against Sporogonic-Stage Parasites Causing Murine Malarias

Arrighi

Nakamura

Miyake

et al. 2002

Antimicrob Agents Chemother

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“…The expression of synthetic peptides in transgenic plants offers advantages over natural analogs, including greater specificity, efficiency at low concentrations and a smaller size compared with natural peptides, without contamination or toxicity for the transgenic plant tissue; furthermore, it better resists degradation compared with natural cecropin (Cary et al, 2000). Transgenic plants from a Populus tremula L. x P. alba L. hybrid that expressed the D4E1 peptide showed a decrease in symptom severity for diseases caused by Agrobacterium tumefaciens and Xanthomonas populi pv.…”

Section: Introductionmentioning

confidence: 99%

“…populi (Mentag et al, 2003). The genetic transformation of tobacco with a gene encoding the synthetic antimicrobial peptide D4E1 controlled the growth of Aspergillus flavus and Verticillium dahliae fungi (Cary et al, 2000); whereas the D4E1 gene expression in cotton plants reduced disease symptoms caused by Thielaviopsis basicola (Rajasekaran et al, 2005). In a recent work, Stover et al (2013) reported excellent results regarding in vitro control by the D4E1 peptide of Proteobacteria, which belong to the same family of HLB-associated bacteria (Ca.…”

Section: Introductionmentioning

confidence: 99%

Genetic transformation of sweet oranges with the D4E1 gene driven by the AtPP2 promoter

Attílio

Filho

Harakava

et al. 2013

Pesq. agropec. bras.

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-The objective of this work was to produce transgenic 'Pêra' and 'Valência' sweet orange plants using the D4E1 gene driven by the Arabidopsis thaliana phloem protein (AtPP2) promoter and to quantify transgene expression in different transformation events. Genetic transformation experiments were carried out with epicotyl segments co-cultivated with Agrobacterium tumefaciens. Six plants from 'Pêra' sweet orange and seven plants from 'Valência' sweet orange were confirmed as different transgenic events by means of the polymerase chain reaction (PCR) and the Southern blot techniques. Transgene expression was quantified using real-time quantitative PCR. D4E1 gene expression levels vary from 5 up to 50 times among different transformation events.Index terms: Citrus sinensis, antimicrobial peptide, tissue-specific promoter. Transformação genética de laranjas doces com o gene D4E1 dirigido pelo promotor AtPP2Resumo -O objetivo deste trabalho foi obter plantas transgênicas de laranja 'Pêra' e 'Valência' por meio do gene D4E1 dirigido pelo promotor Arabidopsis thaliana phloem protein (AtPP2) e quantificar a expressão dos transgenes nos diferentes eventos de transformação. Os experimentos de transformação genética foram realizados com segmentos de epicótilo cocultivados com Agrobacterium tumefaciens. Seis plantas de laranja 'Pêra' e sete plantas de laranja 'Valência' foram confirmadas como distintos eventos transgênicos por meio das técnicas de reação em cadeia da polimerase (PCR) e Southern blot. A expressão dos transgenes foi quantificada por PCR quantitativo em tempo real. Os níveis de expressão do gene D4E1 variam de 5 a 50 vezes entre os diferentes eventos de transformação.Termos para indexação: Citrus sinensis, peptídeo antimicrobiano, promotor tecido-específico.

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“…We utilized this strain as a visual marker to understand the mode of entry, fungal growth, and colonization by A. flavus in cottonseed during boll and seed development. We also utilized this A. flavus strain to successfully evaluate, both qualitatively and quantitatively, resistance to the fungus by cottonseed from transgenic plants expressing the antifungal protein CPO-P [14,15] or the synthetic peptide D4E1 [16][17][18].…”

Section: Introductionmentioning

confidence: 99%

Development of a GFP-Expressing Aspergillus flavus Strain to Study Fungal Invasion, Colonization, and Resistance in Cottonseed

et al. 2008

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Cotton bolls were inoculated with a green fluorescent protein (GFP)-expressing Aspergillus flavus (strain 70) to monitor fungal growth, mode of entry, colonization of cottonseeds, and production of aflatoxins. The GFP strain and the wild-type did not differ significantly in pathogen aggressiveness as indicated by similar reductions in inoculated locule weight. GFP fluorescence was at least 10 times higher than the blue green yellow fluorescence (BGYF) produced in response to infection by A. flavus. The GFP produced by the strain made it possible to identify and monitor specific plant tissues colonized by the fungus. For example, the inner seed coat and cotyledon were colonized by the fungus within 72 h of inoculation and the mode of entry was invariably through the porous chalazal cap in intact seeds. The amount of GFP fluorescence was shown to be an indicator of fungal growth, colonization and, to some extent, aflatoxin production. The A. flavus strain expressing GFP should be very useful for rapidly identifying cotton lines with enhanced resistance to A. flavus colonization developed through genetic engineering or traditional plant breeding. In addition, development of GFP expressing A. flavus strain provides an easy and rapid assay procedure for studying the ecology, etiology, and epidemiology of cotton boll rot caused by A. flavus resulting in aflatoxin contamination.

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Transgenic expression of a gene encoding a synthetic antimicrobial peptide results in inhibition of fungal growth in vitro and in planta

Cited by 111 publications

References 32 publications

Design and Activity of Antimicrobial Peptides against Sporogonic-Stage Parasites Causing Murine Malarias

Design and Activity of Antimicrobial Peptides against Sporogonic-Stage Parasites Causing Murine Malarias

Genetic transformation of sweet oranges with the D4E1 gene driven by the AtPP2 promoter

Development of a GFP-Expressing Aspergillus flavus Strain to Study Fungal Invasion, Colonization, and Resistance in Cottonseed

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