Transgenic chickpeas (Cicer arietinum L.) expressing a sequence-modified cry2Aa gene

Acharjee, Sumita; Sarmah, B. C.; Kumar, Polumetla Ananda; Olsen, Karen; Mahon, R. J.; Moar, William J.; Moore, Andy; Higgins, T. J. V.

doi:10.1016/j.plantsci.2010.02.001

Cited by 78 publications

(35 citation statements)

References 39 publications

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“…Feeding trays consisted of 32 wells (1 cm diameter × 1 cm height). For each line, 16 wells were partially filled with 5 mL of 1% water agar containing 0.1% (w/v) sorbic acid to avoid fungal contamination (Acharjee et al 2010). Approximately 20 mg of fresh leaf material was placed on the agar in each well.…”

Section: Insect Bioassays Using Transgenic Leaf Materialsmentioning

confidence: 99%

Transgenic cowpeas (Vigna unguiculata L. Walp) expressing Bacillus thuringiensis Vip3Ba protein are protected against the Maruca pod borer (Maruca vitrata)

Bett

Gollasch

Moore

et al. 2017

Plant Cell Tiss Organ Cult

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A vip3Ba gene reconstructed for plant expression was used in transforming cowpea via Agrobacterium tumefaciens. Transgenic lines expressing Vip3Ba protein were used in insect feeding trials to assess protection against Maruca and were found to be completely protected from this pest. We propose that the vip-cowpea lines could be combined with existing cry-transgenic cowpea to introgress this additional resistance trait and thus avoid or greatly delay the development of resistance in Maruca.

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Section: Insect Bioassays Using Transgenic Leaf Materialsmentioning

confidence: 99%

Transgenic cowpeas (Vigna unguiculata L. Walp) expressing Bacillus thuringiensis Vip3Ba protein are protected against the Maruca pod borer (Maruca vitrata)

Bett

Gollasch

Moore

et al. 2017

Plant Cell Tiss Organ Cult

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“…Other types of chewing insect pests of legumes can attack the pod and cause considerable damage, for example, Nezara viriolvla in soybean (Brier et al, 1991) or various Helicoverpa spp. such as H. armigera (War et al, 2013) which attack chickpea and pigeon pea (Acharjee et al, 2010) and Maruca vitrata on cowpea (Higgins et al, 2012).…”

Section: G Insectsmentioning

confidence: 99%

“…Most of the transgenic lines generated so far are still at the laboratory stage except the Bt soybean (Miklos et al, 2007) which is close to commercialisation in Brazil, the BGMV resistant bean (Bonfim et al, 2007) which is in advanced field trials in Brazil, the pod borer resistant chickpea (Acharjee et al, 2010) which is in early field trials in India, and the pod borer resistant cowpea (Higgins et al, 2012) which is in multi-location field trials in Nigeria. This is partly due to social (public) and political concerns (lower rate of investment in legume crops compared to other crops such as cotton, canola and maize) that have nearly completely blocked the release of transgenic cultivars of any kind in Europe.…”

Section: G Genetic Transformationmentioning

confidence: 99%

Achievements and Challenges in Legume Breeding for Pest and Disease Resistance

Rubiales

Fondevilla

Cao

et al. 2014

Critical Reviews in Plant Sciences

176

132

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“…Several approaches such as overexpression, RNAi, virus-induced gene silencing and TILLING have been applied for this purpose. Optimised protocols with higher efficiency are already available in chickpea (Acharjee et al 2010). Validation of genes through genetic transformation, RNAi or virus-induced gene silencing is a time-consuming process in legumes, mainly due to the lack of efficient transformation systems in legumes.…”

Section: Transcriptome Sequencing In Chickpeamentioning

confidence: 99%

Genomics-assisted breeding for drought tolerance in chickpea

Thudi

Gaur

Krishnamurthy

et al. 2014

Functional Plant Biol.

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Abstract.Terminal drought is one of the major constraints in chickpea (Cicer arietinum L.), causing more than 50% production losses. With the objective of accelerating genetic understanding and crop improvement through genomicsassisted breeding, a draft genome sequence has been assembled for the CDC Frontier variety. In this context, 544.73 Mb of sequence data were assembled, capturing of 73.8% of the genome in scaffolds. In addition, large-scale genomic resources including several thousand simple sequence repeats and several million single nucleotide polymorphisms, high-density diversity array technology (15 360 clones) and Illumina GoldenGate assay genotyping platforms, high-density genetic maps and transcriptome assemblies have been developed. In parallel, by using linkage mapping approach, one genomic region harbouring quantitative trait loci for several drought tolerance traits has been identified and successfully introgressed in three leading chickpea varieties (e.g. JG 11, Chefe, KAK 2) by using a marker-assisted backcrossing approach. A multilocation evaluation of these marker-assisted backcrossing lines provided several lines with 10-24% higher yield than the respective recurrent parents.Modern breeding approaches like marker-assisted recurrent selection and genomic selection are being deployed for enhancing drought tolerance in chickpea. Some novel mapping populations such as multiparent advanced generation intercross and nested association mapping populations are also being developed for trait mapping at higher resolution, as well as for enhancing the genetic base of chickpea. Such advances in genomics and genomics-assisted breeding will accelerate precision and efficiency in breeding for stress tolerance in chickpea.

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Transgenic chickpeas (Cicer arietinum L.) expressing a sequence-modified cry2Aa gene

Cited by 78 publications

References 39 publications

Transgenic cowpeas (Vigna unguiculata L. Walp) expressing Bacillus thuringiensis Vip3Ba protein are protected against the Maruca pod borer (Maruca vitrata)

Transgenic cowpeas (Vigna unguiculata L. Walp) expressing Bacillus thuringiensis Vip3Ba protein are protected against the Maruca pod borer (Maruca vitrata)

Achievements and Challenges in Legume Breeding for Pest and Disease Resistance

Genomics-assisted breeding for drought tolerance in chickpea

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