Transgenic Chicken, Mice, Cattle, and Pig Embryos by Somatic Cell Nuclear Transfer into Pig Oocytes

Gupta, Mukesh Kumar; Das, Ziban Chandra; Heo, Young-Tae; Joo, Jin Young; Chung, Hak-Jae; Song, Hyuk; Kim, Jaehwan; Kim, Nam‐Hyung; Lee, Hoon Taek; Ko, Dae Hwan; Uhm, Sang Jun

doi:10.1089/cell.2012.0074

Cited by 14 publications

(8 citation statements)

References 51 publications

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“…Over the past several years, many animal trials of MRT have been undertaken ranging from mice to primates to assess safety and ethical questions supporting the many pending requests to initiate human clinical trials in the USA [ 30 , 31 ]. Most recently, the success of primate studies provides strong evidence supporting the effectiveness of MRT in eliminating mitochondrial disease in humans [ 32 – 34 ].…”

Section: Introductionmentioning

confidence: 99%

Overcoming bioethical, legal, and hereditary barriers to mitochondrial replacement therapy in the USA

Pompei

2018

J Assist Reprod Genet

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The purpose of the paper is to explore novel means to overcome the controversial ban in the USA against mitochondrial replacement therapy, a form of IVF, with the added step of replacing a woman’s diseased mutated mitochondria with a donor’s healthy mitochondria to prevent debilitating and often fatal mitochondrial diseases. Long proven effective in non-human species, MRT recently performed in Mexico resulted in the birth of a healthy baby boy. We explore the ethics of the ban, the concerns over hereditability of mitochondrial disease and its mathematical basis, the overlooked role of Mitochondrial Eve, the financial burden of mitochondrial diseases for taxpayers, and a woman’s reproductive rights. We examine applicable court cases, particularly protection of autonomy within the reproductive rights assured by Roe v Wade. We examine the consequences of misinterpreting MRT as genetic engineering in the congressional funding prohibitions causing the MRT ban by the FDA. Allowing MRT to take place in the USA would ensure a high standard of reproductive medicine and safety for afflicted women wishing to have genetically related children, concurrently alleviating the significant financial burden of mitochondrial diseases on its taxpayers. Since MRT does not modify any genome, it falls outside the “heritable genetic modification” terminology of concern to Congress and the FDA. Correcting this terminology, the IOM’s conclusion that MRT is ethical, the continuing normalcy of the first MRT recipient, and increasing public awareness of the promising benefits might be all that is required to modify the FDA’s position on MRT.

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Section: Introductionmentioning

confidence: 99%

Overcoming bioethical, legal, and hereditary barriers to mitochondrial replacement therapy in the USA

Pompei

2018

J Assist Reprod Genet

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“…Studies have used pig oocytes as recipients to reconstruct dog [ 120 , 121 ], cattle [ 120 , 122 ], mouse [ 120 , 122 ], rat [ 31 ], chicken [ 122 ], and whale iSCNT embryos [ 123 ], among others. In fact, it has been postulated that the pig oocyte could be used as a universal recipient due to its high demethylation capacity that is able to support the initiation of nucleoli formation and subsequent early embryo development in several species [ 120 ].…”

Section: Introductionmentioning

confidence: 99%

“…In 2011, for example, Lagutina et al used mSOFaa when using pig oocytes as recipients for multiple species. In the study by Gupta et al (2013), NCSU medium designed for pig oocytes was able to support embryonic development of different donors such as bovine, rodents and chicken [ 122 ]. Studies to reconstruct pig iSNT embryos using pig as nuclear donor are scarce.…”

Section: Introductionmentioning

confidence: 99%

Choosing a culture medium for SCNT and iSCNT reconstructed embryos: from domestic to wildlife species

Cordova¹,

King²,

Mastromonaco³

2017

J Anim Sci Technol

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Over the past decades, in vitro culture media have been developed to successfully support IVF embryo growth in a variety of species. Advanced reproductive technologies, such as somatic cell nuclear transfer (SCNT), challenge us with a new type of embryo, with special nutritional requirements and altered physiology under in vitro conditions. Numerous studies have successfully reconstructed cloned embryos of domestic animals for biomedical research and livestock production. However, studies evaluating suitable culture conditions for SCNT embryos in wildlife species are scarce (for both intra- and interspecies SCNT). Most of the existing studies derive from previous IVF work done in conventional domestic species. Extrapolation to non-domestic species presents significant challenges since we lack information on reproductive processes and embryo development in most wildlife species. Given the challenges in adapting culture media and conditions from IVF to SCNT embryos, developmental competence of SCNT embryos remains low. This review summarizes research efforts to tailor culture media to SCNT embryos and explore the different outcomes in diverse species. It will also consider how these culture media protocols have been extrapolated to wildlife species, most particularly using SCNT as a cutting-edge technical resource to assist in the preservation of endangered species.

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“…Although no offspring have been produced from iSCNT embryos reconstructed with interfamily, interorder, or interclass cell-oocyte couplets, oocytes derived from some species have been frequently used as recipient cytoplasm regardless of the donor cell species to produce hybrid blastocysts for studying the interaction between an oocyte and a somatic nucleus. For example, interfamily SCNT embryos reconstructed by the nuclear transfer of rabbit fetal fibroblasts or cattle fibroblasts into porcine oocytes developed into blastocysts with 5.3% [ 5 ] or 6.3% [ 6 ] efficiency, respectively. Moreover, the success of interorder SCNT blastocysts ranging from 0.6 to 24.0% efficiency were observed when Siberian tiger skin fibroblasts [ 7 ], dog dewclaw fibroblasts [ 8 ], or mouse fibroblasts [ 6 ] were injected into pig oocytes.…”

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confidence: 99%

Suberoylanilide hydroxamic acid during <i>in vitro</i> culture improves development of dog-pig interspecies cloned embryos but not dog cloned embryos

Kim

Choi

et al. 2018

Journal of Reproduction and Development

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This study was conducted to investigate whether the treatment of dog to pig interspecies somatic cell nuclear transfer (iSCNT) embryos with a histone deacetylase inhibitor, to improve nuclear reprogramming, can be applied to dog SCNT embryos. The dog to pig iSCNT embryos were cultured in fresh porcine zygote medium-5 (PZM-5) with 0, 1, or 10 µM suberoylanilide hydroxamic acid (SAHA) for 6 h, then transferred to PZM-5 without SAHA. Although there were no significant differences in cleavage rates, the rates of 5-8-cell stage embryo development were significantly higher in the 10 µM group (19.5 ± 0.8%) compared to the 0 µM groups (13.4 ± 0.8%). Acetylation of H3K9 was also significantly higher in embryos beyond the 4-cell stage in the 10 µM group compared to the 0 or 1 µM groups. Treatment with 10 µM SAHA for 6 h was chosen for application to dog SCNT. Dog cloned embryos with 0 or 10 µM SAHA were transferred to recipients. However, there were no significant differences in pregnancy and delivery rates between the two groups. Therefore, it can be concluded that although porcine oocytes support nuclear reprogramming of dog fibroblasts, treatment with a histone deacetylase inhibitor that supports nuclear reprogramming in dog to pig iSCNT embryos was not sufficient for reprogramming in dog SCNT embryos.

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Transgenic Chicken, Mice, Cattle, and Pig Embryos by Somatic Cell Nuclear Transfer into Pig Oocytes

Cited by 14 publications

References 51 publications

Overcoming bioethical, legal, and hereditary barriers to mitochondrial replacement therapy in the USA

Overcoming bioethical, legal, and hereditary barriers to mitochondrial replacement therapy in the USA

Choosing a culture medium for SCNT and iSCNT reconstructed embryos: from domestic to wildlife species

Suberoylanilide hydroxamic acid during <i>in vitro</i> culture improves development of dog-pig interspecies cloned embryos but not dog cloned embryos

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