Transgenesis Using Nuclear Transfer in Goats

Lazaris, Anthoula; Keyston, Rebecca; Karatzas, C.N.; Keefer, Carol L.

doi:10.1385/1-59745-154-1:213

Cited by 2 publications

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“…The possibility to multiply by cloning the Bperfect functioning^transgenic animal could overcome this problem (Li et al, 2006;Lazaris et al, 2006).…”

Section: Common Problems Related To Transgenesis Transformation Of Famentioning

confidence: 99%

A Brief Overview of Transgenic Farm Animals

Bacci

2007

Vet Res Commun

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Transgenesis offers new possibilities to rapidly modify the genome of living organisms. The application of transgenesis to farm animals faces many problems, more than those observed in the transgenesis of laboratory animals, as there are currently many different techniques available to obtain transgenic animals, which all have problems regarding low efficiency and high costs. When these techniques are applied to farm animals the problems concerning transgenesis are multiplied. Two main techniques, male pronuclear microinjection and sperm mediated gene transfer, utilised in farm animal transgenesis, are briefly presented. The improvement of these techniques and the employment of other biotechnologies such as cloning, could expand the uses of transgenic farm animals for human health.

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“…The possibility to multiply by cloning the Bperfect functioning^transgenic animal could overcome this problem (Li et al, 2006;Lazaris et al, 2006).…”

Section: Common Problems Related To Transgenesis Transformation Of Famentioning

confidence: 99%

A Brief Overview of Transgenic Farm Animals

Bacci

2007

Vet Res Commun

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“…Two important aspects of SCNT technology in producing transgenic livestock are the source and preparation of the somatic donor cells (Lee et al, 2005). Fetal fibroblasts have been used for transgenic goat production because of their rapid growth and potential for multiple cell divisions before becoming senescent in vitro (Lazaris et al, 2006). However, Cho et al (2004) reported that the developmental competence and GFP (green fluorescent protein) expression in bovine transgenic SCNT embryos reconstructed with cumulus cells were significantly higher than those from fetal and ear fibroblasts.…”

Section: Introductionmentioning

confidence: 99%

Cell cycle distribution, cellular viability and mRNA expression of hGCase‐gene‐transfected cells in dairy goat

Wan

Wang

et al. 2010

Cell Biology International

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Nuclear transfer using transgenic donor cells is an efficient way of generating transgenic goats, wherein the preparation of competent transgenic donor cells is the pivotal upstream step. We have measured the efficiency of transfection with a plasmid containing hGCase (human lysosomal acid beta-glucosidase) gene into goat FFC (fetal-derived fibroblast cells), MEC (mammary epithelial cells) and AEFC (adult ear skin-derived fibroblast cells), and the characteristics of cell cycle, apoptosis and chromosome abnormalities after transfection. The expression of genes involved in imprinting [IGF2 (insulin-like growth factor 2), IGF2R (IGF2 receptor)], apoptosis (Bax), stress (heat-shock protein, Hsp70.1), cellular connections [Cx43 (connexin 43)] and DNA methylation [DNMT1 (DNA methyltransferase 1)] in transgenic fetal cells has been investigated. The hGCase transgene was successfully detected in the transfected cell lines, and chromosomal stability remained similar in FFC and transgenic FFC (70.9 compared with 66.8%), whereas a smaller percentage (P<0.05) of cells at G(0)/G(1) in the transgenic FFC, MEC and AEFC (T-FFC, T-MEC and T-AEFC), and higher percentage (P<0.05) of apoptotic cells in T-FFC than the non-transfected controls were detected by flow cytometric analysis. Among the genes tested, the relative expressions of IGF2, IGF2R and transcripts of Cx43 were significantly higher (P<0.05) in T-FFC compared with non-transfected FFC. These novel findings on gene expression in transgenic fetal cells may have certain implications in the biopharming industry and in our understanding the low efficiency of transgenic cloning.

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Transgenesis Using Nuclear Transfer in Goats

Cited by 2 publications

References 0 publications

A Brief Overview of Transgenic Farm Animals

A Brief Overview of Transgenic Farm Animals

Cell cycle distribution, cellular viability and mRNA expression of hGCase‐gene‐transfected cells in dairy goat

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