Transgenesis in farm animals-A review

Mehta, Parul; Sharma, Ankur; Kaushik, Ravinder

doi:10.18805/ag.v38i02.7944

Cited by 6 publications

(5 citation statements)

References 66 publications

(67 reference statements)

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“…The germline transmission of the chimeric animals is tested, and pure transgenic animals are created using various breeding procedures (Miao, 2013). ES cells were previously only used in mice as a means of transgenesis, however, attempts to isolate Embryonic Stem cells in farm animals have also been made (Mehta et al, 2017). However, in these species, this approach fails due to demanding culture conditions, the need to maintain a culture in an undifferentiated state, and the need for complicated genetic manipulation.…”

Section: Embryonic Stem Cells-mediated Transgenesis: Es Cells Are Plu-mentioning

confidence: 99%

A review on molecular breeding techniques: Crucial approach in livestock improvement

Kandel,

Kadariya,

Bohara

et al. 2023

Arch. Agric. Environ. Sci.

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For underdeveloped countries, molecular breeding (MB) has a lot of promise. However, the implementation in developing countries is far from uniform. Livestock improvement programs aim to improve the genetics of domesticated animal populations by selecting males and females who, when mated, will produce progeny that perform better than the current generation's average. The amount of genetic progress made through conventional selection and breeding methods for quantitative traits in livestock is successful, but limitations such as routinely recording phenotypes, animal sacrifice for meat quality traits, recording in particular sex for sex-limited traits, and so on the limit the amount of genetic progress made through conventional selection and breeding methods. Marker-assisted selection (MAS), genome-wide selection (GWS), marker-assisted recurrent selection (MARS), and genome-wide sequencing (GS) are examples of modern breeding procedures. Molecular genetics technology may provide a technique to choose breeding animals at an early age (even embryos), to select for a wide variety of features and to improve the accuracy of forecasting an individual's mature phenotype. This paper examines the challenges and potential of applying molecular breeding techniques to improve livestock in developing countries.

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Section: Embryonic Stem Cells-mediated Transgenesis: Es Cells Are Plu-mentioning

confidence: 99%

A review on molecular breeding techniques: Crucial approach in livestock improvement

Kandel,

Kadariya,

Bohara

et al. 2023

Arch. Agric. Environ. Sci.

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“…In our case, the offspring resulting from injected eggs did not require mating to generate homozygosity. This is an efficient, rapid and low-cost method for preparing genetically edited large animals with identical genetic modification (Zheng et al 2017;Zhou et al 2015;Mehta et al, 2017).…”

Section: 1mentioning

confidence: 99%

Generation of ETV5 Knockout Pigs with CRISPR/Cas9

Zhang¹,

GengYuan²,

Zhou³

et al. 2021

IJAR

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Background: Ets variant factor 5 (ETV5) plays an important regulatory role in mouse Spermatogonial stem cells (SSCs) self-renewal. ETV5 knockout (KO) mice exhibit a progressive loss of SSCs and resulting in a Sertoli cell-only phenotype. The current study was aimed to use gene editing technology to obtain ETV5-KO pigs as a model for studying the apoptosis mechanism of SSCs and further clarify the function of ETV5 gene in pigs.Methods: A gene editing plasmid for the porcine ETV5 gene was constructed, transfected into porcine fetal fibroblasts by electroporation to obtain ETV5-KO cells. ETV5-KO cells were used as donors to prepare ETV5-KO pigs by somatic cell nuclear transfer (SCNT). Testis tissues were collected for hematoxylin and eosin (HE), immunohistochemistry (IHC), RT-PCR testing and blood for ELISA testing from ETV5-KO pig.Result: In the present study, we used the CRISPR/Cas9 system and SCNT to generate homozygous ETV5-KO pigs. We observed 3 phenotypes in these pigs: normal testis development after birth, the SSCs in the seminiferous tubules did not show obviously extinction at sexual maturity and normal spermatogenesis.

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“…At present, mammalian SCNT is imperfect and terminations are frequent, so there are few live births (Mehta et al 2017) and frequent postpartum mortality, and these problems are attributed to placental abnormalities, TE, or placenta dysplasia associated with fetal growth and development during pregnancy. The relevance of the placenta to SCNT embryo development has been reported, but SCNT pig birth weight is also tied to survival.…”

Section: Main Findingmentioning

confidence: 99%

Porcine somatic cell nuclear transfer donor sources affect transplant success: A meta-analysis

Guo¹,

Lv²,

Liu³

et al. 2018

IJAR

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Herd boars, male domestic pigs used for stud, are economically important, and somatic cell nuclear transfer (SCNT) is a promising technology to expand herd boar yields. However, live births are dictated by donor cell source, and fetal donors may offer more advantages than adult donors. A meta-analysis was conducted to better understand how donor sources affect SCNT outcomes. Of the 1,431 records viewed, 10 were selected for review. Blastocyst formation rates, successful pregnancies, and live births were assessed to measure efficacy. SCNT blastocyst formation differed between adult and fetal donors among the studies. SCNT pigs had more malformed fetuses as well, which negatively affected the post-birth mortality. Organs of porcine fetuses are limited by deficiencies of maternal nutrient and growth hormones, which compromise postbirth adaptations. SCNT pregnancy success is neither determined by donor source nor by live births. Live births are also tied to donor age. Embryos from fetal donors are more frequently healthy likely due to less differentiation and less reprogramming of reconstructed embryos. Adult donors in contrast have more cell differentiation and as such accumulate more mutations and damage. This may reduce reconstructed embryo viability. Finally, SCNT efficiency may be improved with more in vitro passages, but more work is required to validate this concept.

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Transgenesis in farm animals-A review

Cited by 6 publications

References 66 publications

A review on molecular breeding techniques: Crucial approach in livestock improvement

A review on molecular breeding techniques: Crucial approach in livestock improvement

Generation of ETV5 Knockout Pigs with CRISPR/Cas9

Porcine somatic cell nuclear transfer donor sources affect transplant success: A meta-analysis

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