Transgenerational safety of nitrogen-doped graphene quantum dots and the underlying cellular mechanism in Caenorhabditis elegans

Zhao, Yunli; Liu, Qian; Shakoor, Shumaila; Gong, Jian; Wang, Dayong

doi:10.1039/c4tx00123k

Cited by 58 publications

(37 citation statements)

References 55 publications

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“…Locomotion behavior of nematodes was assessed by the endpoints of head thrash and body bend as described40. A head thrash was defined as a change in the direction of bending at the mid body.…”

Section: Methodsmentioning

confidence: 99%

Intestinal Insulin Signaling Encodes Two Different Molecular Mechanisms for the Shortened Longevity Induced by Graphene Oxide in Caenorhabditis elegans

Zhao

Yang

Rui

et al. 2016

Sci Rep

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Graphene oxide (GO) has been shown to cause multiple toxicities in various organisms. However, the underlying molecular mechanisms for GO-induced shortened longevity are still unclear. We employed Caenorhabditis elegans to investigate the possible involvement of insulin signaling pathway in the control of GO toxicity and its underlying molecular mechanisms. Mutation of daf-2, age-1, akt-1, or akt-2 gene induced a resistant property of nematodes to GO toxicity, while mutation of daf-16 gene led to a susceptible property of nematodes to GO toxicity, suggesting that GO may dysregulate the functions of DAF-2/IGF-1 receptor, AGE-1, AKT-1 and AKT-2-mediated kinase cascade, and DAF-16/FOXO transcription factor. Genetic interaction analysis suggested the involvement of signaling cascade of DAF-2-AGE-1-AKT-1/2-DAF-16 in the control of GO toxicity on longevity. Moreover, intestinal RNA interference (RNAi) analysis demonstrated that GO reduced longevity by affecting the functions of signaling cascade of DAF-2-AGE-1-AKT-1/2-DAF-16 in the intestine. DAF-16 could also regulate GO toxicity on longevity by functioning upstream of SOD-3, which encodes an antioxidation system that prevents the accumulation of oxidative stress. Therefore, intestinal insulin signaling may encode two different molecular mechanisms responsible for the GO toxicity in inducing the shortened longevity. Our results highlight the key role of insulin signaling pathway in the control of GO toxicity in organisms.

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Section: Methodsmentioning

confidence: 99%

Intestinal Insulin Signaling Encodes Two Different Molecular Mechanisms for the Shortened Longevity Induced by Graphene Oxide in Caenorhabditis elegans

Zhao

Yang

Rui

et al. 2016

Sci Rep

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“…[1][2][3][4] These superiorities make C-dots promising alternatives to heavy metal-based quantum dots for promising applications in bioimaging, 5-7 drug delivery, 8 sensors, [9][10][11][12] and photocatalysis. [1][2][3][4] These superiorities make C-dots promising alternatives to heavy metal-based quantum dots for promising applications in bioimaging, 5-7 drug delivery, 8 sensors, [9][10][11][12] and photocatalysis.…”

Section: Introductionmentioning

confidence: 99%

Oxygen-driven, high-efficiency production of nitrogen-doped carbon dots from alkanolamines and their application for two-photon cellular imaging

Yang

Tian

et al. 2015

RSC Adv.

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A novel oxygen-driven method has been developed for low-cost, large-scale, and high-efficiency production of nitrogen-doped carbon dots (N-C-dots) by bubbling pure oxygen into monoethanolamine (MEA) under heating conditions. We find that the addition of pure oxygen significantly increases the reaction rate, and makes feasible one-pot gram scale fabrication (3.36 g) of highly photoluminescent N-C-dots in a couple of hours (2.0 h). With an instantaneous nucleation and gradual growth mechanism, precise control over the particle size of the N-C-dots from 2.0 to 16.1 nm is achieved by simply prolonging the heating time from 0.5 to 4.0 h. The as-prepared N-C-dots contain aromatic CN heterocycles in the core and have plentiful hydrophilic groups on the surface. Practically, the oxygendriven method can be used to synthesize fluorescent N-C-dots from other alkanolamines such as diethanolamine (DEA) and triethanolamine (TEA), which shows general universality. Due to the strong up-conversion photoluminescence, good aqueous dispersibility, high photostability, excellent biocompatibility, and low cytotoxicity, the N-C-dots are demonstrated to be promising two-photon probes for high contrast bioimaging applications.

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“…The mean defecation cycle length was analyzed as described60. Individual nematodes were examined for their fixed number of cycles.…”

Section: Methodsmentioning

confidence: 99%

Wnt Ligands Differentially Regulate Toxicity and Translocation of Graphene Oxide through Different Mechanisms in Caenorhabditis elegans

Zhi

Ren

et al. 2016

Sci Rep

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In this study, we investigated the possible involvement of Wnt signals in the control of graphene oxide (GO) toxicity using the in vivo assay system of Caenorhabditis elegans. In nematodes, the Wnt ligands, CWN-1, CWN-2, and LIN-44, were found to be involved in the control of GO toxicity. Mutation of cwn-1 or lin-44 gene induced a resistant property to GO toxicity and resulted in the decreased accumulation of GO in the body of nematodes, whereas mutation of cwn-2 gene induces a susceptible property to GO toxicity and an enhanced accumulation of GO in the body of nematodes. Genetic interaction assays demonstrated that mutation of cwn-1 or lin-44 was able to suppress the susceptibility to GO toxicity shown in the cwn-2 mutants. Loss-of-function mutations in all three of these Wnt ligand genes resulted in the resistance of nematodes to GO toxicity. Moreover, the Wnt ligands might differentially regulate the toxicity and translocation of GO through different mechanisms. These findings could be important in understanding the function of Wnt signals in the regulation of toxicity from environmental nanomaterials.

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Transgenerational safety of nitrogen-doped graphene quantum dots and the underlying cellular mechanism in Caenorhabditis elegans

Abstract: Nitrogen-doped graphene quantum dots (N-GQDs) are safe for environmental release.

Cited by 58 publications

References 55 publications

Intestinal Insulin Signaling Encodes Two Different Molecular Mechanisms for the Shortened Longevity Induced by Graphene Oxide in Caenorhabditis elegans

Intestinal Insulin Signaling Encodes Two Different Molecular Mechanisms for the Shortened Longevity Induced by Graphene Oxide in Caenorhabditis elegans

Oxygen-driven, high-efficiency production of nitrogen-doped carbon dots from alkanolamines and their application for two-photon cellular imaging

Wnt Ligands Differentially Regulate Toxicity and Translocation of Graphene Oxide through Different Mechanisms in Caenorhabditis elegans

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