Transgenerational epigenetic reprogramming of early embryos: a mechanistic model

Spadafora, Corrado

doi:10.1093/eep/dvaa009

Cited by 14 publications

(8 citation statements)

References 104 publications

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“…EVs act as cell‐to‐cell communication nanoparticles (Mathieu et al, 2019) containing micro RNA and small RNA molecules that mediate cell signaling and epigenetics (Park et al, 2019; Saadeldin et al, 2014; Spadafora, 2020). Results showed the expression of SOD1 in the OEC EVs that supports the evidence that oviductal secretion provide antioxidative defense to the early embryos (Lee et al, 2021; Li & Winuthayanon, 2017).…”

Section: Discussionmentioning

confidence: 99%

Oviduct epithelial cells‐derived extracellular vesicles improve preimplantation developmental competence of in vitro produced porcine parthenogenetic and cloned embryos

Fang

Tanga

Bang

et al. 2021

Molecular Reproduction Devel

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Extracellular vesicles (EVs) carry bioactive cargoes involved in the early preimplantation development. This study investigated the effects of EVs obtained from an oviductal epithelial cell (OEC) conditioned medium on the developmental competence of in parthenogenetic activation (PA) and somatic cell nuclear transfer (SCNT) porcine embryos. The OEC‐EV‐treated group showed significant increases in blastocyst formation and hatching rates compared to the control group (40.8% ± 2.2% and 20.1% ± 2.1% vs. 24.9% ± 2.0% and 5.3% ± 1.1%; p < 0.05), respectively. The 7 day OEC‐EVs treatment group significantly increased blastocyst formation rate than the 3 day and 0 day‐groups (45.0 ± 0.8 vs. 33.0 ± 0.7 and 26.7 ± 0.5; p < 0.05), respectively. SCNT revealed that the OEC‐EV increased blastocyst formation rate compared to that of oviductal fluid EVs (OF‐EVs) (35.4% ± 1.4% vs. 29.3% ± 1.3%; p < 0.05). Reactive oxygen species levels, apoptosis, and blastocyst lipid content were significantly decreased in the OEC‐EVs group compared with the control group. OEC‐EV group showed a significantly decreased BAX and increased BCL2, SOD1, POU5F1, SOX2, NANOG, GATA6, PNPLA2, LIPE, and MGLL gene expression than the control group (p < 0.05). In conclusion, OEC‐EVs supplementation in embryo culture media improved the quality of porcine embryos, potentially helping porcine‐cloned embryonic development possibly through transfer of messenger RNA and proteins to the early embryos.

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Section: Discussionmentioning

confidence: 99%

Oviduct epithelial cells‐derived extracellular vesicles improve preimplantation developmental competence of in vitro produced porcine parthenogenetic and cloned embryos

Fang

Tanga

Bang

et al. 2021

Molecular Reproduction Devel

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“…The flow is capable of crossing the Weismann barrier, facilitated by circulating vesicles. This process ultimately results in the possibility to pass specific epigenetic traits on to the offspring [ 144 ].…”

Section: Embryo Development and Critical Windowmentioning

confidence: 99%

Impact of Endocrine Disruptors upon Non-Genetic Inheritance

Montjean

Neyroud

Yéfimova

et al. 2022

IJMS

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Similar to environmental factors, EDCs (endocrine-disrupting chemicals) can influence gene expression without modifying the DNA sequence. It is commonly accepted that the transgenerational inheritance of parentally acquired traits is conveyed by epigenetic alterations also known as “epimutations”. DNA methylation, acetylation, histone modification, RNA-mediated effects and extracellular vesicle effects are the mechanisms that have been described so far to be responsible for these epimutations. They may lead to the transgenerational inheritance of diverse phenotypes in the progeny when they occur in the germ cells of an affected individual. While EDC-induced health effects have dramatically increased over the past decade, limited effects on sperm epigenetics have been described. However, there has been a gain of interest in this issue in recent years. The gametes (sperm and oocyte) represent targets for EDCs and thus a route for environmentally induced changes over several generations. This review aims at providing an overview of the epigenetic mechanisms that might be implicated in this transgenerational inheritance.

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“…Transgenerational effects can include parental effects (e.g. egg provisioning and transfer of sperm cell cytoplasmic components) and epigenetics (Dai et al 2020; Spadafora 2020; Venney et al 2020). For example, brook trout ( S. fontinalis ) offspring incurred more differentially methylated regions due to transgenerational warm-acclimation than with within-generation warm-acclimation (Venney et al 2022).…”

Section: Discussionmentioning

confidence: 99%

“…TGP occurs through modulation of gene expression through non-genetic inheritance, which includes parental effects and the transfer of epigenetic factors such as patterns of DNA methylation and histone modification, and microRNA activity (Dai et al 2020; Spadafora 2020; Venney et al 2020). TGP can potentially be beneficial when a parent’s environment coincides with what their offspring are expected to experience (Bernatchez 2016; Ashe et al 2021).…”

Section: Introductionmentioning

confidence: 99%

Transcriptomic responses to within- and transgenerational environmental warming in a cold-adapted salmonid

Penney

Burness

Wilson

2022

Preprint

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Cold-adapted species are particularly threatened by climate change as rates of environmental warming outpace the ability of many populations adapt. Recent evidence suggest that transgenerational thermal plasticity may play a role in the response of cold-adapted organisms to long-term changes in temperature. Using RNA sequencing, we explored differential gene expression of lake trout (Salvelinus namaycush), a cold-adapted species, to examine the molecular processes that respond to elevated temperatures under conditions of within-generation (offspring) and transgenerational (parental) warm-acclimation. We hypothesized that genes associated with metabolism, growth and thermal stress/tolerance would be differentially expressed in juvenile lake trout offspring depending on their own acclimation temperature and that of their parents. We found enriched pathways for thermal stress, signaling processes, immune function, and transcription regulation, although the specific genes and direction of differential expression (up- or downregulation) depended on the combination of offspring and parental thermal rearing conditions. While parental warm-acclimation did have a transgenerational effect on gene expression in their offspring, within-generation warm-acclimation had a larger effect on differential expression, enriching more pathways. We provide evidence of the transgenerational response to warming at the transcriptional level in lake trout, which should be useful for future studies of transcriptomics and plasticity in these and other cold-adapted species.

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Transgenerational epigenetic reprogramming of early embryos: a mechanistic model

Cited by 14 publications

References 104 publications

Oviduct epithelial cells‐derived extracellular vesicles improve preimplantation developmental competence of in vitro produced porcine parthenogenetic and cloned embryos

Oviduct epithelial cells‐derived extracellular vesicles improve preimplantation developmental competence of in vitro produced porcine parthenogenetic and cloned embryos

Impact of Endocrine Disruptors upon Non-Genetic Inheritance

Transcriptomic responses to within- and transgenerational environmental warming in a cold-adapted salmonid

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