Transgene introgression in crop relatives: molecular evidence and mitigation strategies

Kwit, Charles; Moon, Hong S.; Warwick, S. I.; Stewart, C. Neal

doi:10.1016/j.tibtech.2011.02.003

Cited by 93 publications

(90 citation statements)

References 90 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, no fitness effect was detected for the clustering region on LG3. QTL research, such as ours, will therefore prove as a valuable tool to give a first indication of regions in the crop genome less likely to introgress before any transgene is inserted (Kwit et al 2011;Stewart et al 2003), but such results do have to be verified in the field under selective conditions (Hartman et al 2012).…”

Section: Implications For Crop Breedingmentioning

confidence: 99%

“…A particular concern is possible negative ecological effects, such as increased invasiveness of the wild relative (Chapman and Burke 2006;Stewart et al 2003). In order to minimize chances of transgene escape, mitigation strategies have been proposed where the transgene is in linkage with an allele that is selected against in the wild and therefore is more likely to be purged from the wild population (Gressel 1999;Kwit et al 2011;Stewart et al 2003). Locating domestication-related QTL might be a way to pinpoint genomic areas where the crop allele confers a fitness disadvantage to hybrid individuals.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

QTL analysis reveals the genetic architecture of domestication traits in Crisphead lettuce

Hartman

Hooftman

Schranz

et al. 2012

Genet Resour Crop Evol

View full text Add to dashboard Cite

The genetic architecture of crop domestication is generally characterized by three trends: relatively few genomic regions with major QTL effects are involved, QTL are often clustered, and alleles derived from the crop do not always contribute to the crop phenotype. We have investigated the genetic architecture of lettuce using a recombinant inbred line population from a cross between a crop Lactuca sativa ('Salinas') and its wild relative L. serriola. Few genomic regions with major QTL, plus various intermediate QTL, largely control the transition from wild to cultivated Crisphead lettuce. Allelic effects of all major QTL were in the expected direction, but there were intermediate QTL where the crop contributed to the wild phenotype and vice versa. We found two main regions with clusters of QTL, one on linkage group 3, where the crop allele induced lower seed output, another on linkage group 7, where the crop allele caused a delay in flowering time. Potentially, knowledge of genetic changes due to the domestication could be relevant for the chance that a transgene inserted in a crop genome will spread to wild relatives due to hitchhiking effects. If a transgene would be inserted in one of these regions, background selection on the crop alleles may lead to a reduced fitness of hybrids with the transgene. QTL research on the effects of domestication genes can thus indicate regions in the crop genome that are less likely to introgress, although these still need to be verified under field conditions.

show abstract

Section: Implications For Crop Breedingmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

QTL analysis reveals the genetic architecture of domestication traits in Crisphead lettuce

Hartman

Hooftman

Schranz

et al. 2012

Genet Resour Crop Evol

View full text Add to dashboard Cite

show abstract

“…Much attention has been paid to the exchange of potentially adaptive variation between crop plants and their wild relatives (Kwit et al, 2011;Ellstrand et al, 2013;Warschefsky et al, 2014). For example, adaptive introgression of transgenes conferring herbivore resistance from crop plants to wild relatives has been demonstrated in artificial hybrids of rice (Yang et al, 2011) and sunflower (Helianthus annuus; Snow et al, 2003).…”

Section: Adaptive Introgressionmentioning

confidence: 99%

Hybridization in Plants: Old Ideas, New Techniques

2016

View full text Add to dashboard Cite

Hybridization has played an important role in the evolution of many lineages. With the growing availability of genomic tools and advancements in genomic analyses, it is becoming increasingly clear that gene flow between divergent taxa can generate new phenotypic diversity, allow for adaptation to novel environments, and contribute to speciation. Hybridization can have immediate phenotypic consequences through the expression of hybrid vigor. On longer evolutionary time scales, hybridization can lead to local adaption through the introgression of novel alleles and transgressive segregation and, in some cases, result in the formation of new hybrid species. Studying both the abundance and the evolutionary consequences of hybridization has deep historical roots in plant biology. Many of the hypotheses concerning how and why hybridization contributes to biological diversity currently being investigated were first proposed tens and even hundreds of years ago. In this Update, we discuss how new advancements in genomic and genetic tools are revolutionizing our ability to document the occurrence of and investigate the outcomes of hybridization in plants.

show abstract

“…Flow of genes into wild or weedy relatives can occur also from conventionally bred, non-GE crops [128][129][130], even causing negative environmental consequences [131]. However, transgenesis may create a higher level of uncertainty in terms of environmental risk.…”

Section: Flow Of Recombinant Dnamentioning

confidence: 99%

“…Breeders can take advantage of gametic incompatibility, which can block fertilization of non-GE corn kernels by GE pollen [145]. Additional options exist for mitigating the risk of transgene flow [130,144,146,147]. Since in some crops, the risk of transgene flow may be greatly minimized but remain non-zero, some may argue for GE to be limited to non-transgenic applications.…”

Section: Flow Of Recombinant Dnamentioning

confidence: 99%

Genetic Engineering and Sustainable Crop Disease Management: Opportunities for Case-by-Case Decision-Making

Vincelli

2016

Sustainability

View full text Add to dashboard Cite

Genetic engineering (GE) offers an expanding array of strategies for enhancing disease resistance of crop plants in sustainable ways, including the potential for reduced pesticide usage. Certain GE applications involve transgenesis, in some cases creating a metabolic pathway novel to the GE crop. In other cases, only cisgenessis is employed. In yet other cases, engineered genetic changes can be so minimal as to be indistinguishable from natural mutations. Thus, GE crops vary substantially and should be evaluated for risks, benefits, and social considerations on a case-by-case basis. Deployment of GE traits should be with an eye towards long-term sustainability; several options are discussed. Selected risks and concerns of GE are also considered, along with genome editing, a technology that greatly expands the capacity of molecular biologists to make more precise and targeted genetic edits. While GE is merely a suite of tools to supplement other breeding techniques, if wisely used, certain GE tools and applications can contribute to sustainability goals.

show abstract

Transgene introgression in crop relatives: molecular evidence and mitigation strategies

Cited by 93 publications

References 90 publications

QTL analysis reveals the genetic architecture of domestication traits in Crisphead lettuce

QTL analysis reveals the genetic architecture of domestication traits in Crisphead lettuce

Hybridization in Plants: Old Ideas, New Techniques

Genetic Engineering and Sustainable Crop Disease Management: Opportunities for Case-by-Case Decision-Making

Contact Info

Product

Resources

About