Transgene and gene suppression: telling us something new?

Dougherty, William G.; Parks, T. Dawn

doi:10.1016/0955-0674(95)80096-4

Cited by 207 publications

(142 citation statements)

References 47 publications

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“…In contrast, lines BS1 and BS9 showed wild-type levels of endogenous AIPTR2-B mRNA. We propose that reduction of AIPTR2-B expression in line BS2 is due to co-suppression (Dougherty and Parks, 1995). As shown below, consistent with this idea, the phenotypes of the BS2 line resemble that of the antisense transgenic plants.…”

Section: Transformed With the Sense Constructsupporting

confidence: 76%

Antisense Expression of the Peptide Transport Gene AtPTR2-B Delays Flowering and Arrests Seed Development in Transgenic Arabidopsis Plants

et al. 1997

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Previously, we identified a peptide transport gene, AfPTRZ-B, from Arabidopsis fhaliana that was constitutively expressed in all plant organs, suggesting an important physiological role in plant growth and development. To evaluate the function of this transporter, transgenic Arabidopsis plants were constructed expressing antisense or sense AfPTRZ-B. Genomic Southern analysis indicated that four independent antisense and three independent sense AtPTRZ-B transgenic lines were obtained, which was confirmed by analysis of the segregation of the kanamycin resistance gene carried on the T-DNA. RNA blot data showed that the endogenous AfPJRZ-B mRNA levels were significantly reduced in transgenic leaves and flowers, but not in transgenic roots. Consistent with this reduction in endogenous AfPTRZ-B mRNA levels, all four antisense lines and one sense line exhibited significant phenotypic changes, including late flowering and arrested seed development. These phenotypic changes could be explained by a defect in nitrogen nutrition due to the reduced peptide transport activity conferred by AtPTR2-B. These results suggest that AtPTRZ-B may play a general role in plant nutrition. l h e AtPTR2-B gene was mapped to chromosome 2, which is closely linked to the restriction fragment length polymorphism marker 111246.

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Section: Transformed With the Sense Constructsupporting

confidence: 76%

Antisense Expression of the Peptide Transport Gene AtPTR2-B Delays Flowering and Arrests Seed Development in Transgenic Arabidopsis Plants

et al. 1997

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“…Transgenes in plants often undergo silencing, and in some instances the endogenous homologues of these genes also suffer inactivation, a phenomenon referred to as cosuppression (reviewed by Flavell, 1994;Dougherty and Parks, 1995;Matzke and Matzke, 1995). In many cases silencing seems to be post-transcriptional.…”

mentioning

confidence: 99%

Post-transcriptional regulation of sex determination in Caenorhabditis elegans: widespread expression of the sex-determining gene fem-1 in both sexes.

Gaudet

VanderElst

Spence

1996

MBoC

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The fem-1 gene of C. elegans is one of three genes required for all aspects of male development in the nematode. Current models of sex determination propose that the products of the fem genes act in a novel signal-transduction pathway and that their activity is regulated primarily at the post-translational level in somatic tissues. We analyzed the expression of fem-1 to determine whether it revealed any additional levels of regulation. Both XX hermaphrodites and XO males express fem-1 at approximately constant levels throughout development. Somatic tissues in hermaphrodites adopt female fates, but they nonetheless expressfem-1 mRNA and FEM-1 protein, suggesting that the regulation of fem-i activity is post-transcriptional and probably post-translational. A compact promoter directs functional expression of fem-1 transgenes, as assayed by their masculinizing activity infem-i mutants. Activity also requires any two or more introns, suggesting that splicing may enhance fem-1 expression. The minimal noncoding sequences required for activity of fem-1 transgenes suffice to direct expression of a fem-i::lacZ reporter gene in all somatic tissues in both sexes. Many fem-1 transgenes, including those that rescue male somatic development in fem-1 mutants, paradoxically feminize the germline. We suggest that they do so by interfering with the germline expression of the endogenous fem-1 gene.

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“…PTGS is a common plant defense response active against diverse viruses and likely to play an important role in cross-protection [6,7]. Low temperature treatment has been found effective to induce mutation [13].…”

Section: Discussionmentioning

confidence: 99%

“…It was only this model that could explain the protection offered by naked RNA or viroids, and also, it justify why high level of homology is required to induce this response. Several lines of evidences indicate that RNA-based protection is derived from a nucleotide (nt) sequence-specific host defense mechanism, termed as post-transcriptional gene silencing (PTGS) that targets viral RNAs for destruction [6,7] demonstrating that PTGS is a common plant defense response active against diverse viruses and likely to play an important role in cross-protection. Data presented by different scientists also revealed that pre-expression of viral proteins play an important role in cross-protection.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of Low Temperature Induced Mutants of Cucumber green mottle mosaic virus for Cross-protection in Cucurbits

Ali¹,

Ahmad²,

Nishigawa³

et al. 2015

J Plant Pathol Microbiol

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Transgene and gene suppression: telling us something new?

Cited by 207 publications

References 47 publications

Antisense Expression of the Peptide Transport Gene AtPTR2-B Delays Flowering and Arrests Seed Development in Transgenic Arabidopsis Plants

Antisense Expression of the Peptide Transport Gene AtPTR2-B Delays Flowering and Arrests Seed Development in Transgenic Arabidopsis Plants

Post-transcriptional regulation of sex determination in Caenorhabditis elegans: widespread expression of the sex-determining gene fem-1 in both sexes.

Evaluation of Low Temperature Induced Mutants of Cucumber green mottle mosaic virus for Cross-protection in Cucurbits

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