Transgelin (TAGLN) Regulates IQGAP1 and Alters the Functions of Breast Cancer Cells

Abstract

Several studies have been conducted on the transgelin (TAGLN) protein and its critical role in cancer biology. However, the regulation of this protein and the way in which this regulation is correlated with the functions of IQ motif-containing GTPase-activating protein 1 (IQGAP1) in MDA-MB231 cells, remain unclear. We generated stable TAGLN-knockdown and TAGLN-overexpressing cells. These cells, along with their control counterparts, were cultured in the presence or absence of 17-AAG. The different cell groups were then subjected to functional assays to assess proliferation, chemotaxis, and invasion. TAGLN regulation was found to affect the efficacy of 17-AAG. The ability of TAGLN to influence the levels of IQGAP1 and its binding partners altered the critical functions of breast cancer cells. Therefore, the altered functionality of MDA-MB-231 cells, as a consequence of TAGLN regulation, is correlated with IQGAP1 signaling.

Structure–activity relationship of gallic acid from Paeonia lactiflora and its synthetic analogs against human breast cancer cells

“…The phenolic acids GA (1) and MG (2) showed weak cytotoxicity, with IC 50 values of 85.0 and 94.9 μM in MCF-7 cells and 99.7 and 160 μM in MDA-MB-231 cells, respectively. The monoterpene glucosides oxypaeoniflorin (3), albiflorin (4), and paeoniflorin (5), and the phenol paeonol (7) were not cytotoxic to either MCF-7 or MDA-MB-231 cells. The IC 50 values of PGG (6) in MCF-7 and MDA-MB-231 cells were 20.1 and 14.1 μM, respectively.…”

“…To discovery new promising chemotherapy, drug-targetable proteins in oncogenic signaling pathway of BC have been investigated and natural products are a source of a considerable number of clinical anticancer drugs or lead compounds with potential for cancer treatment. [2][3][4][5][6] Recently, it is reported that protein tyrosine phosphatase 1B (PTP1B) is an important oncogene in BC and markedly promotes BC activities in the HER2-positive cell (MCF-7) and in the TNBC cell line (MDA-MB-231). 7 MCF-7 cell line is ER-positive and carries the oncogenic E545 mutation of PIK3CA gene, which provide phosphatidylinositol 3-kinase (PI3K) signaling related to cell survival.…”

“…Therefore, new BC therapeutic options are still needed to maximize the efficacy and minimize the side effects of chemotherapy. To discovery new promising chemotherapy, drug‐targetable proteins in oncogenic signaling pathway of BC have been investigated and natural products are a source of a considerable number of clinical anticancer drugs or lead compounds with potential for cancer treatment 2–6 . Recently, it is reported that protein tyrosine phosphatase 1B (PTP1B) is an important oncogene in BC and markedly promotes BC activities in the HER2‐positive cell (MCF‐7) and in the TNBC cell line (MDA‐MB‐231) 7 .…”