Transfusion reaction due to <i>Klebsiella pneumoniae</i>‐contaminated red blood cells: a case report

Warnke, Philipp; Kiefel, V.; Schäffler, Holger; Podbielski, Andreas

doi:10.1111/tme.12078

Cited by 2 publications

(2 citation statements)

References 10 publications

(12 reference statements)

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“…Skin flora S. epidermidis was selected as one of the most common blood product contaminants while the 5 Gram-negative species were chosen since they have been shown to either grow in RBCs [7] or been implicated in adverse transfusion reactions involving contaminated RBC units [8,9,10]. …”

Section: Methodsmentioning

confidence: 99%

Changing the 30-min Rule in Canada: The Effect of Room Temperature on Bacterial Growth in Red Blood Cells

Ramirez‐Arcos

Kou

Ducas

et al. 2016

Transfus Med Hemother

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Background: To maintain product quality and safety, the ‘30-min rule' requires the discard of red blood cells (RBCs) that are exposed to uncontrolled temperatures for more than 30 min. Recent studies suggest this rule may safely be extended to a 60-min rule. Methods: A pool-and-split design study (N = 4) was run in parallel at Canadian Blood Services (SAGM RBCs) and Héma-Québec (AS-3 RBCs). RBCs were spiked with ∼1 colony-forming unit/ml of mesophilic and psychrophilic bacteria. Control units remained in storage at 1-6 °C for 42 days. Test 30 (T30) and T60 units were exposed to room temperature (RT) six times during storage, each time for 30 and 60 min, respectively. Bacterial proliferation was monitored. Results: Mesophilic bacteria do not proliferate in RBCs. The growth of psychrophilic bacteria is not significantly different in RBCs exposed for 30 or 60 min to RT (p < 0.05). Conclusion: The study findings were the final evidence to support extension from a 30-min rule to a 60-min rule in Canada.

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Section: Methodsmentioning

confidence: 99%

Changing the 30-min Rule in Canada: The Effect of Room Temperature on Bacterial Growth in Red Blood Cells

Ramirez‐Arcos

Kou

Ducas

et al. 2016

Transfus Med Hemother

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“…Similarly, there was one case of a fatal transfusion reaction involving RBCC contaminated with Pseudomonas koreensis documented in the SHOT report of 2009 [6]. Klebsiella pneumoniae has also been implicated in septic transfusion events involving contaminated RBCC [7][8][9]. More recently, Frati and colleagues published a case report of a transfusion-transmitted septic reaction with a fatal outcome in Italy caused by a RBC unit contaminated with Yersinia enterocolitica [10].…”

Section: Introductionmentioning

confidence: 99%

Establishment of transfusion‐relevant bacteria reference strains for red blood cells

et al. 2020

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Background and objectives Red blood cell concentrates (RBCC) are susceptible to bacterial contamination despite cold storage. A reliable evaluation of strategies to minimize the risk of RBCC-associated bacterial transmission requires the use of suitable reference bacteria. Already existing Transfusion-Relevant Bacteria Reference Strains (TRBRS) for platelet concentrates fail to grow in RBCC. Consequently, the ISBT TTID, Working Party, Bacterial Subgroup, conducted an international study on TRBRS for RBCC.Materials and methods Six bacterial strains (Listeria monocytogenes PEI-A-199, Serratia liquefaciens PEI-A-184, Serratia marcescens PEI-B-P-56, Pseudomonas fluorescens PEI-B-P-77, Yersinia enterocolitica PEI-A-105, Yersinia enterocolitica PEI-A-176) were distributed to 15 laboratories worldwide for enumeration, identification, and determination of growth kinetics in RBCC at days 7, 14, 21, 28, 35 and 42 of storage after low-count spiking (10-25 CFU/RBCC).Results Bacterial proliferation in RBCC was obtained for most strains, except for S. marcescens, which grew only at 4 of 15 laboratories. S. liquefaciens, S. marcescens, P. fluorescens and the two Y. enterocolitica strains reached the stationary phase between days 14 and 21 of RBCC storage with a bacterial 692This is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.concentration of approximately 10 9 CFU/ml. L. monocytogenes displayed slower growth kinetics reaching 10 6 -10 7 CFU/ml after 42 days. ConclusionThe results illustrate the importance of conducting comprehensive studies to establish well-characterized reference strains, which can be a tool to assess strategies and methods used to ameliorate blood safety. The WHO Expert Committee on Biological Standardization adopted the five successful strains as official RBCC reference strains. Our study also highlights the relevance of visual inspection to interdict contaminated RBC units.

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Transfusion reaction due to Klebsiella pneumoniae‐contaminated red blood cells: a case report

Cited by 2 publications

References 10 publications

Changing the 30-min Rule in Canada: The Effect of Room Temperature on Bacterial Growth in Red Blood Cells

Changing the 30-min Rule in Canada: The Effect of Room Temperature on Bacterial Growth in Red Blood Cells

Establishment of transfusion‐relevant bacteria reference strains for red blood cells

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