Transforming growth factor β1polymorphism and serum levels in Egyptian patients with interstitial lung diseases

Azmy, Rania; Helbawy, Rana Helmy El; Dawood, Ashraf; Dahdaouh, Sami El

doi:10.1016/j.ejcdt.2015.12.001

Cited by 2 publications

(1 citation statement)

References 32 publications

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“…However, advanced gene-sequencing technology discovered that ethnicity influences the distribution of genetic variations in populations and has a major impact on the connection between alleged genetic markers and cancer risk (11). The application of molecular and ELISA testing has been found to improve the effectiveness of identifying and evaluating lung cancer patients with NSCLC (25).…”

Section: Introductionmentioning

confidence: 99%

TGFβ1, SMAD2, CTNNβ1, and Wnt3a gene mutational status and serum concentrations in individuals with non-small cell lung cancer

Hemn Abdalla Omer,

Kawa Amin

2023

Cell Mol Biol (Noisy-le-grand)

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The objective of the current investigation was to investigate the diagnostic utility of the serum concentrations and mutational status of TGFβ1, SMAD2, CTNNβ1, and Wnt3a. and the expression levels of human-related genes in patients with non-small cell lung cancer (NSCLC). The serum concentrations were determined using the ELISA technique, and PCR for genotype variations of TGFβ1, SMAD2, CTNNβ1, and Wnt3a were examined using Sanger sequencing in tissue samples obtained from 93 patients with NSCLC and 84 healthy individuals for blood, and 20 Formalin Fixed Paraffin Embedded (FFPE) from normal samples dissected adjacent to the tumour. The findings of the current investigation indicate that individuals diagnosed with NSCLC exhibited significant elevation in the serum levels of CEA and CYFRA21-1, as well as TGFβ1, SMAD2, CTNNβ1, and Wnt3a. In total, 325 mutations in four trialled genes (243 mutations in TGFβ1, 24 mutations in SMAD2,47 mutation Wnt3a and 11 mutations in CTNNβ1) were identified in patients with NSCLC. Furthermore, all mutations were recorded in adenocarcinoma, not squamous and normal adjacent tumour cells. CYFRA21-1 and CEA are more significant between NSCLC and HC, gender, and NSCLC types (p<0.001). In detail, TGFβ1 exhibited the highest rate of mutations among other genes and three types of genomic mutations. Elevated levels and genetic polymorphisms of TGFβ1, SMAD2, CTNNβ1, and Wnt3a may play crucial functions in the pathogenesis and angiogenesis of non-small cell lung cancer (NSCLC). These biomarkers might play a role in future immunologic response and pharmacologically targeted NSCLC therapy.

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Section: Introductionmentioning

confidence: 99%

TGFβ1, SMAD2, CTNNβ1, and Wnt3a gene mutational status and serum concentrations in individuals with non-small cell lung cancer

Hemn Abdalla Omer,

Kawa Amin

2023

Cell Mol Biol (Noisy-le-grand)

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Role of transforming growth factor beta in assessment of severity of hypersensitivity pneumonitis: a single center study

EL Hinnawy,

Salem,

Ahmed

et al. 2024

Egypt J Bronchol

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Background Hypersensitivity pneumonitis (HP) is a more frequently diagnosed picture of diffuse parenchymal lung disease. It is an inflammation of the lung tissue, provoked by immune mechanisms, which happens to prone individuals as a reaction to a wide range of antigens. There are different degrees of fibrosis and inflammation. A group of extracellular mediators both proinflammatory and profibrotic claimed to be involved in the pathogenesis of HP. Among these mediators, a significant role is played by transforming growth factor-beta (TGF-β). Aim Correlation between the severity of hypersensitivity pneumonitis and the serum level of TGF beta. Patients and methods Sixty subjects were included in the study who were classified into 30 patients newly diagnosed with hypersensitivity pneumonitis and 30 healthy subjects served as controls. All the participants were subjected to complete history taking, physical examination, spirometry, 6-min walk distance test, HRCT, and serum levels of TGF-β. Results The serum level of TGF beta is elevated in newly diagnosed HP cases (fibrotic and non-fibrotic) in relation to control participants showing statistical significance p value < 0.001, and the serum level of TGF beta in the fibrotic group of HP patients is more than that in non-fibrotic group with statistical significance p value 0.012. Conclusion The serum level of transforming growth factor can be used in the assessment of the severity of hypersensitivity pneumonitis as regards the intensity of lung parenchymal changes.

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Transforming growth factor β1polymorphism and serum levels in Egyptian patients with interstitial lung diseases

Cited by 2 publications

References 32 publications

TGFβ1, SMAD2, CTNNβ1, and Wnt3a gene mutational status and serum concentrations in individuals with non-small cell lung cancer

TGFβ1, SMAD2, CTNNβ1, and Wnt3a gene mutational status and serum concentrations in individuals with non-small cell lung cancer

Role of transforming growth factor beta in assessment of severity of hypersensitivity pneumonitis: a single center study

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