Transforming Growth Factor β1/Smad4 Signaling Affects Osteoclast Differentiation via Regulation of miR-155 Expression

Zhao, Hongying; Zhang, Jun; Shao, Haiyu; Liu, Jianwen; Jin, Mengran; Chen, Jinping; Huang, Yanlei

doi:10.14348/molcells.2017.2303

Cited by 39 publications

(31 citation statements)

References 52 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Other miRNAs associated to bone, whose function were not previously characterized in human (i.e., hsa-miR-155-5p 56 , 57 , hsa-miR-181a-5p 58 , hsa-miR-30c-5p 59 , hsa-miR-378a-3p 60 , 61 , and hsa-miR-93-3p 62 ) were modulated in trained subjects. Taken together these results suggest that chronic PA (i.e., training) alters the expression profile of several miRNAs which mark the skeletal muscle and bone adaptation to such a stimulus.…”

Section: Discussionmentioning

confidence: 81%

Normalization strategies differently affect circulating miRNA profile associated with the training status

Faraldi

Gomarasca

Sansoni

et al. 2019

Sci Rep

122

109

View full text Add to dashboard Cite

MicroRNAs are fine regulators of the whole-body adaptive response but their use as biomarkers is limited by the lack of standardized pre- and post-analytical procedures. This work aimed to compare different normalization approaches for RT-qPCR data analyses, in order to identify the most reliable and reproducible method to analyze circulating miRNA expression profiles in sedentary and highly-trained subjects. As the physically active status is known to affect miRNA expression, they could be effective biomarkers of the homeostatic response. Following RNA extraction from plasma, a panel of 179 miRNAs was assayed by RT-qPCR and quantified by applying different normalization strategies based on endogenous miRNAs and exogenous oligonucleotides. hsa-miR-320d was found as the most appropriate reference miRNA in reducing the technical variability among the experimental replicates and, hence, in highlighting the inter-cohorts differences. Our data showed an association between the physically active status and specific skeletal muscle- and bone-associated circulating miRNAs profiles, revealing that established epigenetic modifications affect the baseline physiological status of these tissues. Since different normalization strategies led to different outputs, in order to avoid misleading interpretation of data, we remark the importance of the accurate choice of the most reliable normalization method in every experimental setting.

show abstract

Section: Discussionmentioning

confidence: 81%

Normalization strategies differently affect circulating miRNA profile associated with the training status

Faraldi

Gomarasca

Sansoni

et al. 2019

Sci Rep

122

109

View full text Add to dashboard Cite

show abstract

“…Osteoclast differentiation was demonstrated to be modulated by RANKL-RANK signaling in cooperation with nuclear factor of activated T cells (NFAT) [ 43 ] and TGFβ [ 24 , 44 , 45 ]. RANK was also demonstrated to interact with the intracellular molecule TNF receptor–associated factor 6 (TRAF6), which plays an important role in the several signaling pathways, including NF-κB, p38 kinase, and c-Jun N-terminal kinase (JNK) [ 46 ].…”

Section: Discussionmentioning

confidence: 99%

Therapeutic and Preventive Effects of Osteoclastogenesis Inhibitory Factor on Osteolysis, Proliferation of Mammary Tumor Cell and Induction of Cancer Stem Cells in the Bone Microenvironment

Futakuchi

Nitanda

Ando

et al. 2018

IJMS

View full text Add to dashboard Cite

Background: We examined the effects of recombinant human osteoclastogenesis inhibitory factor (hOCIF) on osteolysis, proliferation of mammary tumor cells, and induction of cancer stem cells (CSCs) in the tumor-bone and tumor-subcutaneous microenvironments (TB- and TS-microE). Methods: Mouse mammary tumor cells were transplanted onto the calvaria or into a subcutaneous lesion of female mice, creating a TB-microE and a TS-microE, and the mice were then treated with hOCIF. To investigate the preventive effects of hOCIF, mice were treated with hOCIF before tumor cell implantation onto the calvaria (Pre), after (Post), and both before and after (Whole). The number of CSCs and cytokine levels were evaluated by IHC and ELISA assay, respectively. Results: hOCIF suppressed osteolysis, and growth of mammary tumors in the TB-microE, but not in the TS-microE. In the Pre, Post, and Whole groups, hOCIF suppressed osteolysis, and cell proliferation. hOCIF increased mouse osteoprotegrin (mOPG) levels in vivo, which suppressed mammary tumor cell proliferation in vitro. These preventive effects were observed in the dose-dependent. hOCIF did not affect the induction of CSCs in either microenvironment. Conclusion: While receptor activator of NF-κB ligand (RANKL) targeting therapy may not affect the induction of CSCs, RANKL is a potential target for prevention as well as treatment of breast cancer bone metastasis.

show abstract

“…miR-155 upregulation and TGF-β1 overexpression accelerate EMT and promote cell migration and invasion, thereby inducing hepatocellular carcinoma progression ( 26 ). Furthermore, TGF-β1 has been reported to upregulate miR-155 expression to alter osteoclast differentiation ( 27 ). Based on these findings, the present study hypothesized that miR-155 induction by TGF-β1 may serve a potential role in CRS pathogenesis.…”

Section: Discussionmentioning

confidence: 99%

miR‑155‑5p downregulation inhibits epithelial‑to‑mesenchymal transition by targeting SIRT1 in human nasal epithelial cells

et al. 2020

View full text Add to dashboard Cite

epithelial-to-mesenchymal transition (eMT) in nasal epithelial cells is involved with tissue remodeling of nasal polyps. The present study investigated the molecular mechanisms through which mir-155-5p regulated eMT in chronic rhinosinusitis (crS). Patients were divided into the following groups: crSsnP, crS without nasal polyposis group, crSwnP, crS with nasal polyposis and controls. The expression of transforming growth factor (TGF)-β1, eMT markers, sirtuin 1 (SirT1) and mir-155-5p were determined by western blotting and reverse transcription-quantitative Pcr. cell morphology following TGF-β1 treatment in the presence of mir-155-5p inhibitors or controls was observed under a microscope. Target genes and potential binding sites between mir-155-5p and SirT1 were predicted by TargetScan and confirmed using dual-luciferase reporter assay. In patients with crS, the expression levels of e-cadherin were downregulated and the expression levels of TGF-β1, mesenchymal markers and mir-155-5p were upregulated. additionally, these changes in expression levels were reduced or increased to a greater extent in the crSwnP group compared with the crSsnP group. Furthermore, TGF-β1 expression promoted eMT in human nasal epithelial cells (Hnepcs) and upregulated mir-155-5p expression. These effects were reversed by mir-155-5p inhibitors. additionally, SirT1 was predicted as a target gene of mir-155-5p. downregulation of mir-155-5p upregulated epithelial marker expression and downregulated mesenchymal marker expression by regulating SirT1. Therefore, the downregulation of mir-155-5p inhibited eMT in Hnepcs by targeting SirT1.

show abstract

Transforming Growth Factor β1/Smad4 Signaling Affects Osteoclast Differentiation via Regulation of miR-155 Expression

Cited by 39 publications

References 52 publications

Normalization strategies differently affect circulating miRNA profile associated with the training status

Normalization strategies differently affect circulating miRNA profile associated with the training status

Therapeutic and Preventive Effects of Osteoclastogenesis Inhibitory Factor on Osteolysis, Proliferation of Mammary Tumor Cell and Induction of Cancer Stem Cells in the Bone Microenvironment

miR‑155‑5p downregulation inhibits epithelial‑to‑mesenchymal transition by targeting SIRT1 in human nasal epithelial cells

Contact Info

Product

Resources

About