Transforming Growth Factor-β Signaling in Stem Cells and Cancer

Mishra, Lopa; Derynck, Rik; Mishra, Bibhuti

doi:10.1126/science.1118389

Cited by 189 publications

(170 citation statements)

References 28 publications

Supporting

Mentioning

164

Contrasting

Unclassified

Order By: Relevance

“…The relative overexpression of genes in cluster A tumours, discussed here and shown in Table 2a, indicated divergent mesenchymal differentiation potential, which is similar to that of pluripotent mesenchymal stem cells (MSCs) (Mishra et al, 2005). Consistent with the histological features of ILNR-derived WTs that often have various ectopic mesenchymal elements, the Figure 6) are in the second column from the right.…”

Section: Ctnnb1 Mutations and Nuclear Accumulation Of B-catenin In Wtssupporting

confidence: 67%

Canonical WNT signalling determines lineage specificity in Wilms tumour

et al. 2009

View full text Add to dashboard Cite

Wilms tumours (WTs) have two distinct types of histology with or without ectopic mesenchymal elements, suggesting that WTs arise from either the mesenchymal or epithelial nephrogenic lineages. Regardless of the presence or absence of CTNNB1 mutations, nuclear accumulation of b-catenin is often observed in WTs with ectopic mesenchymal elements. Here, we addressed the relationship between the WNT-signalling pathway and lineage in WTs by examining CTNNB1 and WT1 mutations, nuclear accumulation of b-catenin, tumour histology and gene expression profiles. In addition, we screened for mutations in WTX, which has been proposed to be a negative regulator of the canonical WNT-signalling pathway. Unsupervised clustering analysis identified two classes of tumours: mesenchymal lineage WNT-dependent tumours, and epithelial lineage WNT-independent tumours. In contrast to the mesenchymal lineage specificity of CTNNB1 mutations, WTX mutations were surprisingly observed in both lineages. WTX-mutant WTs with ectopic mesenchymal elements had nuclear accumulation of b-catenin, upregulation of WNT target genes and an association with CTNNB1 mutations in exon 7 or 8. However, epithelial lineage WTs with WTX mutations had no indications of active WNT signalling, suggesting that the involvement of WTX in the WNT-signalling pathway may be lineage dependent, and that WTX may have an alternative function to its role in the canonical WNT-signalling pathway.

show abstract

Section: Ctnnb1 Mutations and Nuclear Accumulation Of B-catenin In Wtssupporting

confidence: 67%

Canonical WNT signalling determines lineage specificity in Wilms tumour

et al. 2009

View full text Add to dashboard Cite

show abstract

“…Transforming growth factor-b superfamily genes have a broad range of biological effects and control processes during development and tissue repair (Mishra et al, 2005). Incubation of murine embryonic SVZ cells with Bmp 2, Bmp 4, and Bmp 7 promotes astroglial lineage commitment, and this effect is not mimicked by application of other members of TGF-b superfamily such as TGFb1 and activin A (Gross et al, 1996).…”

Section: Discussionmentioning

confidence: 99%

Stroke Induces Gene Profile Changes Associated with Neurogenesis and Angiogenesis in Adult Subventricular Zone Progenitor Cells

Liu¹,

Zhang²,

Zhang³

et al. 2006

J Cereb Blood Flow Metab

104

100

View full text Add to dashboard Cite

Neural progenitor cells in the subventricular zone (SVZ) of the lateral ventricular wall give rise to new neurons throughout rodent life. Ischemic stroke induces angiogenesis and neurogenesis. Using laser capture microdissection (LCM) in combination with microarrays containing approximately 400 known genes associated with stem cells and angiogenesis, we investigated gene profiles of SVZ cells in the adult mouse subjected to middle cerebral artery occlusion. Our data revealed that nonstroke SVZ cells expressed sets of genes that are important for neural progenitor cell proliferation, differentiation, and migration. In addition, stroke SVZ cells expressed many genes involved in neurogenesis during embryonic development but were not detected in nonstroke SVZ cells. Stroke upregulated genes were verified by real-time reverse transcriptase-polymerase chain reaction and immunostaining. These data indicate that adult SVZ cells recapture embryonic molecular signals after stroke and provide insight into the molecular mechanisms, which regulate the biological function of neural progenitor cells in the SVZ of adult rodent brain under physiological and stroke conditions.

show abstract

“…Early markers of differentiation appeared to be present at higher levels in BG01V as compared to any of the hESC lines examined, although hESC specific genes continued to be expressed at high levels (see Additional file 4). In particular, the Wnt pathway and the TGFβ signaling pathway ( Figure 6D), both of which involved in the early process of differentiation [24,25], appeared to be activated (Additional file 4), suggesting that the role of growth factors and signaling in these early events cannot be readily studied in this cell line.…”

Section: Smaller But Distinct Differences Among Undifferentiated Hescmentioning

confidence: 99%