Transforming growth factor-? inhibits CCAAT/enhancer-binding protein expression and PPAR? activity in unloaded bone marrow stromal cells

Ahdjoudj, Souhila; Kaabeche, Karim; Holy, Xavier; Fromigué, Olivia; Modrowski, Dominique; Zérath, E.; Marie, Pierre J.

doi:10.1016/j.yexcr.2004.09.013

Cited by 37 publications

(39 citation statements)

References 61 publications

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“…This correlated with an inhibition of adipogenesis and an enhanced chondrogenesis . Independent studies have shown that TGF-b treatment inhibited the induction of the adipogenic transcription factors C/EBPa, C/ EBPb, and PPARg during skeletal unloading in rodent models [Ahdjoudj et al, 2002[Ahdjoudj et al, , 2005. These results suggest that similar networking occurs between the Wnt pathway and other TGF-b related cytokines such as the BMPs.…”

Section: Outside-in Signalingmentioning

confidence: 89%

Playing with bone and fat

Gimble

Zvonic

Floyd

et al. 2006

J of Cellular Biochemistry

474

367

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The relationship between bone and fat formation within the bone marrow microenvironment is complex and remains an area of active investigation. Classical in vitro and in vivo studies strongly support an inverse relationship between the commitment of bone marrow-derived mesenchymal stem cells or stromal cells to the adipocyte and osteoblast lineage pathways. In this review, we focus on the recent literature exploring the mechanisms underlying these differentiation events and discuss their implications relevant to osteoporosis and regenerative medicine.

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Section: Outside-in Signalingmentioning

confidence: 89%

Playing with bone and fat

Gimble

Zvonic

Floyd

et al. 2006

J of Cellular Biochemistry

474

367

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“…Jump training strongly upregulated the expression of these osteogenic factors. Transforming growth factor (TGF)-inhibits the activity of PPAR by promoting its phosphorylation (Hu et al1996;Adams et al 1997;Ahdjoudj et al 2005). BMPs, members of the TGF super-family, strongly inhibit adipogenic diVerentiation and promote osteoblastic diVerentiation (Wozney et al 1988;Sen et al 2008).…”

Section: Discussionmentioning

confidence: 99%

“…Bone marrow cells from the rat tibiae were Xushed out from the proximal metaphysis (Ahdjoudj et al 2002(Ahdjoudj et al , 2005. Total RNA was extracted using an RNeasy mini kit (Qiagen, Tokyo, Japan).…”

Section: Rna Extraction and Cdna Synthesismentioning

confidence: 99%

“…Bone volume and marrow fat volume are inversely proportional (Morrie 1985;Justesen et al 2001;Moerman et al 2004;Benayahu et al 2000;Ahdjoudj et al 2002Ahdjoudj et al , 2005Botolin et al 2005). Osteoporosis and age-related osteopenia are associated with an increase in marrow fat volume (Morrie 1985;Justesen et al 2001;Moerman et al 2004).…”

Section: Introductionmentioning

confidence: 99%

“…Osteoporosis and age-related osteopenia are associated with an increase in marrow fat volume (Morrie 1985;Justesen et al 2001;Moerman et al 2004). Various osteopenic animals have also shown an increase in marrow fat volume (Benayahu et al 2000;Ahdjoudj et al 2002Ahdjoudj et al , 2005Botolin et al 2005). Conversely, osteogenic physical exercise decreases marrow fat volume (David et al 2007;Menuki et al 2008).…”

Section: Introductionmentioning

confidence: 99%

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Upregulation of osteogenic factors induced by high-impact jumping suppresses adipogenesis in marrow but not adipogenic transcription factors in rat tibiae

et al. 2010

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Jump training is a high-impact training regimen that increases bone volume in young bones. The aim of our study was to determine whether downregulation of adipogenesis that is associated with upregulation of osteogenesis is detected after jump training in growing rat tibiae. Four-week-old rats were jump trained for 1, 2, or 4 weeks for 5 days/week, and the height of jumping progressively increased to 35 cm. We performed morphometry to directly quantitate changes in bone volume and marrow adipocyte distribution in tibiae after the jump training. We also examined changes in the expression of osteogenic and adipogenic transcription factor proteins and mRNAs after the jump training. Four weeks of jump training induced an increase in trabecular bone volume, which was associated with the recruitment of runt-related transcription factor 2 expressing cells, as well as a decrease in marrow fat volume. However, peroxisome proliferator-activated receptor-gamma2 protein and mRNA expression levels did not change after high-impact jump training. The mRNA expression levels of the adipocyte differentiation genes CCAAT/enhancer-binding proteins (C/EBPs)alpha, C/EBPbeta, and C/EBPdelta also showed no change during the training period in jump-trained rats. We suggest that the levels of osteogenic factors that were upregulated by mechanical loading from high-impact jumping suppress adipogenesis in marrow rather than adipogenic transcription factors.

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Deciphering adipose development: Function, differentiation and regulation

Guo,

Wang,

et al. 2024

Developmental Dynamics

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The overdevelopment of adipose tissues, accompanied by excess lipid accumulation and energy storage, leads to adipose deposition and obesity. With the increasing incidence of obesity in recent years, obesity is becoming a major risk factor for human health, causing various relevant diseases (including hypertension, diabetes, osteoarthritis and cancers). Therefore, it is of significance to antagonize obesity to reduce the risk of obesity‐related diseases. Excess lipid accumulation in adipose tissues is mediated by adipocyte hypertrophy (expansion of pre‐existing adipocytes) or hyperplasia (increase of newly‐formed adipocytes). It is necessary to prevent excessive accumulation of adipose tissues by controlling adipose development. Adipogenesis is exquisitely regulated by many factors in vivo and in vitro, including hormones, cytokines, gender and dietary components. The present review has concluded a comprehensive understanding of adipose development including its origin, classification, distribution, function, differentiation and molecular mechanisms underlying adipogenesis, which may provide potential therapeutic strategies for harnessing obesity without impairing adipose tissue function.

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Transforming growth factor-? inhibits CCAAT/enhancer-binding protein expression and PPAR? activity in unloaded bone marrow stromal cells

Cited by 37 publications

References 61 publications

Playing with bone and fat

Playing with bone and fat

Upregulation of osteogenic factors induced by high-impact jumping suppresses adipogenesis in marrow but not adipogenic transcription factors in rat tibiae

Deciphering adipose development: Function, differentiation and regulation

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