Transforming growth factor alpha in developing rats

Brown, P. I.; Lam, Robert W.; Lakshmanan, J.; Fisher, Delbert A.

doi:10.1152/ajpendo.1990.259.2.e256

Cited by 40 publications

(34 citation statements)

References 28 publications

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“…The lack of ontogenic changes in TGF-a mRNA concentrations was unexpected because we have observed age-dependent changes in renal TGF-a immunoreactivity (27). This peptide-mRNA discrepancy could relate to mRNA stability, protein storage pools, or developmental changes in the renal TGF-a precursor processing enzyme(~).…”

Section: Discussionmentioning

confidence: 82%

Ontogeny of Epidermal Growth Factor, Transforming Growth Factor-α, Epidermal Growth Factor Receptor, and Thyroid Hormone Receptor RNA Levels in Rat Kidney and Changes in Those Levels Induced by Early Thyroxine Treatment

et al. 1992

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ABSTRAm. Ontogenic changes in the mRNA levels of epidermal growth factor (EGF), transforming growth factor-a, EGF receptor, and thyroid hormone receptor (rerbA) were examined in developing rat kidneys. The mRNA levels of both EGF and thyroid hormone receptor rose dramatically during the postnatal period with the rise in thyroid hormone receptor message preceding the rise in EGF message. In addition, we examined renal mRNA levels in 1-wk-old rats treated with thyroxine (T4) from birth through d 6. Neonatal T4 treatment augmented the renal mRNA levels of EGF but decreased the levels of EGF-receptor and transforming growth factor-a. T4 treatment did not significantly affect the levels of renal mRNA for thyroid hormone receptor. Although the EGF and transforming growth factor-a peptides are similar and interact with the same receptor, our findings indicate that these homologous growth factors are regulated differently during development. In addition, hormones that influence growth and development, such as T4, may function both as positive and negative regulators of growth factor expression. (Pediatr Res 31: 330-334, 1992) born rodents (5, 6), and it has been proposed that EGF mediates these developmental effects of thyroid hormone (7).Thyroid hormones also have been shown to increase EGF as well as EGF mRNA levels in developing rodent kidneys (8). To more fully understand the interrelations among these polypeptide growth factors and thyroid hormones in developing rodent kidney, cDNA probes for EGF, TGF-a, EGF-R, and thyroid hormone receptor (r-erbA-P) were used to examine normal rat kidneys taken from animals ranging in age from 21 d postconception to adult. Northern blots were prepared and used to quantify changes in the relative amount of each message during development. The ontogenic changes in message levels were examined with respect to the normal postnatal increase in T4 secretion. In addition, newborn animals were injected with T4 for the first 6 d of life to prematurely increase circulating adult T4 levels. Northern blots prepared from the kidneys of treated and control animals were examined to determine the effect of T4 treatment on the message levels of EGF, TGF-a, EGF receptor, and thyroid hormone receptor. MATERIALS AND METHODS AbbreviationsSprague-Dawley rats were obtained from Simonson Laboratories. Date-bred rats from the same source provided the fetal EGF, epidermal growth factor and neonatal animals used in the study. The animals were TGF-a, transforming growth factor-a maintained in accordance with the NIH Guide for the Care and EGF-R, epidermal growth factor receptor Use of Laboratory Animals. For the T4 treatment studies, new-SSC, saline sodium citrate born rats were treated with T4 (0.4 yg/g body wt/d) from the day T3, triiodothronine of birth through d 6 of life; kidneys were collected on d 7. After T4, thyroxine decapitation, rat kidney tissues were removed, quickly frozen in poly A+, polyadenylated a bath of liquid nitrogen, and stored at -70°C. Before RNA extraction, the pooled kidney tissue...

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Section: Discussionmentioning

confidence: 82%

Ontogeny of Epidermal Growth Factor, Transforming Growth Factor-α, Epidermal Growth Factor Receptor, and Thyroid Hormone Receptor RNA Levels in Rat Kidney and Changes in Those Levels Induced by Early Thyroxine Treatment

et al. 1992

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“…TGF-alpha is a potent stimulator of normal hepatocyte proliferation, which plays important roles during hepatic development and regeneration (1,19).…”

Section: Introductionmentioning

confidence: 99%

Immunohistochemical Localization of Transforming Growth Factor Alpha in Chemically Induced Rat Hepatocellular Carcinomas with Reference to Differentiation and Proliferation

et al. 2000

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“…Similar to the kidney recovering from acute injury, EGF-R binding and receptor tyrosine phosphorylation increase during days 16-21 of rodent and murine gestation (22). However, no study has been able to prove conclusively that either EGF or transforming growth factor ␣ are involved in metanephric development (19,(23)(24)(25)(26).…”

Section: Introductionmentioning

confidence: 99%

Production of heparin binding epidermal growth factor-like growth factor in the early phase of regeneration after acute renal injury. Isolation and localization of bioactive molecules.

Sakai¹,

Zhang²,

Homma³

et al. 1997

J. Clin. Invest.

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We have recently reported that heparin-binding epidermal growth factor-like growth factor (HB-EGF) mRNA is induced in the rat kidney after acute ischemic injury. The present studies were designed to investigate whether bioactive HB-EGF protein is also produced in response to renal injury induced by either ischemia/reperfusion or aminoglycosides. Heparin-binding proteins were purified from kidney homogenates by heparin affinity column chromatography using elution with a 0.2-2.0 M gradient of NaCl. A single peak of proteins that eluted at 1.0-1.2 M NaCl was detected in the postischemic kidney within 6 h of injury. This eluate fraction stimulated DNA synthesis in quiescent Balb/c3T3, RIE, and NRK-52E cell lines, all of which are responsive to the epidermal growth factor family of mitogenic proteins. The EGF receptor of A431 cells was also tyrosine phosphorylated by this eluate peak. Furthermore, immunoblotting with a polyclonal antibody against rat HB-EGF indicated that the eluate peak contained immunoreactive proteins of 22 and 29 kD mol wt, consistent with the reported sizes of the secreted form and membrane anchored form of HB-EGF, respectively. Immunohistochemical studies revealed that HB-EGF was produced predominantly in distal tubules in kidneys injured either by ischemia/reperfusion or aminoglycoside administration. We also found that during metanephric development immunoreactive HB-EGF was detected in the ureteric bud as early as E14.5 and persisted in structures arising from the ureteric bud throughout embryogenesis. These results suggest that in response to acute injury, HB-EGF is produced predominantly in distal tubules and that endogenous HB-EGF may be an important growth factor involved in renal epithelial cell repair, proliferation, and regeneration in the early stages of recovery after acute renal injury, as well as in nephrogenesis.

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Transforming growth factor alpha in developing rats

Cited by 40 publications

References 28 publications

Ontogeny of Epidermal Growth Factor, Transforming Growth Factor-α, Epidermal Growth Factor Receptor, and Thyroid Hormone Receptor RNA Levels in Rat Kidney and Changes in Those Levels Induced by Early Thyroxine Treatment

Ontogeny of Epidermal Growth Factor, Transforming Growth Factor-α, Epidermal Growth Factor Receptor, and Thyroid Hormone Receptor RNA Levels in Rat Kidney and Changes in Those Levels Induced by Early Thyroxine Treatment

Immunohistochemical Localization of Transforming Growth Factor Alpha in Chemically Induced Rat Hepatocellular Carcinomas with Reference to Differentiation and Proliferation

Production of heparin binding epidermal growth factor-like growth factor in the early phase of regeneration after acute renal injury. Isolation and localization of bioactive molecules.

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