“…Split-GAL4 enhancer combinations identified in D. melanogaster have been used to target pIP10 in D. yakuba (Ding et al, 2019), suggesting that the split-GAL4 reagents we have identified may be useful for studying homologous circuits across Drosophila species. Testing anatomical hypotheses will require comparative connectomics, which should be facilitated by recent advances in EM (Dorkenwald et al;Lesser et al, 2023;Marin et al, 2023;Phelps et al, 2021a;Scheffer et al, 2020;Schlegel et al, 2023aSchlegel et al, , 2023bSheridan et al, 2022;Xu et al, 2017) and in expansion microscopy (Lillvis et al, 2022).…”