Transformation systems, gene silencing and gene editing technologies in oomycetes

Ghimire, Bikal; Saraiva, Márcia; Andersen, Christian B.; Gogoi, A. K.; Saleh, Mona; Zic, Nicola; West, Pieter van; Brurberg, May Bente

doi:10.1016/j.fbr.2021.11.001

Cited by 11 publications

(11 citation statements)

References 126 publications

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“…El conocimiento generado por el RNA-seq contribuye a la identificación de nuevos genes blancos o dianas para uso en edición genética como también en la búsqueda de perfiles genéticos que se traduzcan en el desarrollo de plantas resistentes a patógenos (Jaiswal et al, 2019;Bashar y Hanif, 2021;Ghimire et al, 2022). Además, la secuenciación directa de ARN es uno de los mayores retos en el área de la fitopatología.…”

Section: Las Aplicaciones Del Análisis De Transcriptomasunclassified

Aplicación Potencial Del Análisis Transcriptómico en Los Planes De Manejo De Insectos Plaga Que Afectan El Sector Agropecuario De Panamá

Atencio-Valdespino,

Sánchez-Galán,

Ramos-Delgado

et al. 2023

Trop Subtrop Agroecosyst

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Background. Insect pests, as well as other arthropods, constitute a constant threat to public health and food security and food sovereignty in a country. Although various techniques are currently used for integrated pest management, the search for new management alternatives is constant. In this sense, transcriptome analysis, which consists of the analysis of gene expression profiles of certain living organisms, could provide new alternatives. Objective. To determine the potential applications of transcriptomic analysis against insect pests in the agricultural sector in Panama. Methodology. The study was developed through the search for relevant information on transcriptome analysis applied within entomology. Search was performed in SciELO (Scientific Electronic Library Online), Dialnet, Web of Science, Springer Link, Ciencia.Science.gov, Scopus, Google Schoolar and ERIC (Institute of Education Sciences). Main findings. The analysis of transcriptomes is a tool to understand different biological, physiological, and molecular aspects of insects and other arthropods identified as pests in the Republic of Panama that put at risk the agricultural production of the country. Implications. Harmful arthropods have become more relevant in recent years due to climate change, invasive species and anthropogenic factors that increase the populations of these pest in Panama. Conclusion. The results indicate that transcriptomic analysis generates information about the existence of candidate genes, including those of harmful arthropods and natural enemies as predators, parasitoids and entomopathogenic microorganisms that could be used as targets to reduce the damage caused by arthropods to the agricultural sector.

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Section: Las Aplicaciones Del Análisis De Transcriptomasunclassified

Aplicación Potencial Del Análisis Transcriptómico en Los Planes De Manejo De Insectos Plaga Que Afectan El Sector Agropecuario De Panamá

Atencio-Valdespino,

Sánchez-Galán,

Ramos-Delgado

et al. 2023

Trop Subtrop Agroecosyst

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“…Nevertheless, electroporation, biolistics, PEG-mediated protoplast transfection, and Agrobacterium-mediated transformation methods have been successfully used in selected oomycetes. Pioneering studies using RNA interference and CRISPR/Cas in Phytophthora species and Aphanomyces invadans showed the potential to modulate their development and virulence using reverse genetics tools [revived in ( Ghimire et al., 2022 )].…”

Section: Membrane-anchored Calpains As Targets For Future Functional ...mentioning

confidence: 99%

Membrane-anchored calpains – hidden regulators of growth and development beyond plants?

Šafranek,

Shumbusho,

Johansen

et al. 2023

Front. Plant Sci.

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Calpains are modulatory proteases that modify diverse cellular substrates and play essential roles in eukaryots. The best studied are animal cytosolic calpains. Here, we focus on enigmatic membrane-anchored calpains, their structural and functional features as well as phylogenetic distribution. Based on domain composition, we identified four types of membrane-anchored calpains. Type 1 and 2 show broad phylogenetic distribution among unicellular protists and streptophytes suggesting their ancient evolutionary origin. Type 3 and 4 diversified early and are present in brown algae and oomycetes. The plant DEK1 protein is the only representative of membrane-anchored calpains that has been functionally studied. Here, we present up to date knowledge about its structural features, putative regulation, posttranslational modifications, and biological role. Finally, we discuss potential model organisms and available tools for functional studies of membrane-anchored calpains with yet unknown biological role. Mechanistic understanding of membrane-anchored calpains may provide important insights into fundamental principles of cell polarization, cell fate control, and morphogenesis beyond plants.

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“…Multiple studies have shown that the mutation of Barley Mildew Resistance Locus O (Mlo), which encodes a membraneassociated protein necessary for the fungal pathogen to penetrate the host epidermal cells, results in plant immunity to powdery mildew [70,71]. CRISPR has also been utilized for treating oomycete infection [72]. The papaya plant mutant for a functional cysteine protease inhibitor (PpalEPIC8) was developed using the CRISPR-Cas9 method, increasing the plant's resistance to the destructive oomycete disease against Phytophthora palmivora [73].…”

Section: Crispr/cas Studies On Different Typesmentioning

confidence: 99%

ROLE OF CRISPR/Cas IN CONTROLLING BIOTIC STRESS IN BLACKPEPPER

K S

2023

JASR

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Black pepper (Piper nigrum L.), the king of spices is one of the most important traditional spices cultivated all over world. Over the past 15 years, there has been a noticeable decline in crop production and area due to biotic and abiotic stress. Despite the efforts made to develop and select a number of black pepper varieties with high yield potential and disease tolerance, the situation has not improved in a decade. Quick wilt caused by Phytopthora capsici, one of the major soil-borne fungi can destroy black pepper crops and cause heavy loss in the plantations. All plant parts are vulnerable to infection, which results in significant decrease in gene expression, thereby inducing heavy mortality rate. Different resistant varieties are raised based on different breeding programs to control the disease and helps in maintaining black pepper production. Such labor-intensive, unfocused breeding initiatives that take so much time and effort cannot keep up with the needs for higher crop production. Currently, a novel gene editing technique known as the clustered regularly interspaced short palindromic repeats (CRISPR)/Cas technology, has succeeded in enhancing crop quality that increase yield, quality as well as to improve resistance to biotic and abiotic stresses. The main objective of this review is to identify the role of CRISPR/Cas technology in controlling the quick wilt fungi by genome editing. Recent improvements in CRISPR/Cas genome editing allow for effective targeted modification in the majority of crops, which promises to hasten crop development, especially in commercially important crop like black pepper.

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Transformation systems, gene silencing and gene editing technologies in oomycetes

Cited by 11 publications

References 126 publications

Aplicación Potencial Del Análisis Transcriptómico en Los Planes De Manejo De Insectos Plaga Que Afectan El Sector Agropecuario De Panamá

Aplicación Potencial Del Análisis Transcriptómico en Los Planes De Manejo De Insectos Plaga Que Afectan El Sector Agropecuario De Panamá

Membrane-anchored calpains – hidden regulators of growth and development beyond plants?

ROLE OF CRISPR/Cas IN CONTROLLING BIOTIC STRESS IN BLACKPEPPER

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