Transformation ofBrassica oleracea L.: a critical review

Puddephat, I. J.; Riggs, T. J.; Fenning, T. M.

doi:10.1007/bf00564197

Cited by 56 publications

(21 citation statements)

References 105 publications

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“…Several of the tested cultivars have demonstrated the highest regeneration frequency from leaves of any cauliflower cultivar yet reported. Seedling hypocotyls are preferred for regeneration and transformation of brassicas (Puddephat et al, 1996;Cardoza and Stewart, 2004), however, there are few reports of shoot regeneration from B. oleracea leaves. Regeneration frequencies from B. oleracea leaves have been reported as 0 (Bhalla and de Weerd, 1999), 19% (Cheng et al, 2001), 31% (Ovesná et al, 1993), 50% (Dunwell, 1981) and 79% (Cao and Earle, 2003) but there have been few reports of shoot number per explant.…”

Section: Shoot Regeneration From Leaf Explants Of Cauliflower Cultivarsmentioning

confidence: 99%

Regeneration from leaf explants and protoplasts of Brassica oleracea var. botrytis (cauliflower)

Chikkala

Nugent

Dix

et al. 2009

Scientia Horticulturae

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Section: Shoot Regeneration From Leaf Explants Of Cauliflower Cultivarsmentioning

confidence: 99%

Regeneration from leaf explants and protoplasts of Brassica oleracea var. botrytis (cauliflower)

Chikkala

Nugent

Dix

et al. 2009

Scientia Horticulturae

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“…The methods of choice for transformation of Brassica species have been based upon the natural genetic engineering capabilities of Agrobacterium tumefaciens and A. rhizogenes (Poulsen 1996;Puddephat et al 1996). Similar transformation mechanisms operate in both Agrobacterium species, mediated by genes conserved in the vir (virulence) region of the large extra-chromosomal plasmids found in virulent bacteria of both species (Tepfer 1990).…”

Section: Introductionmentioning

confidence: 99%

The effects of anther culture and plant genetic background on Agrobacterium rhizogenes-mediated transformation of commercial cultivars and derived doubled-haploid Brassica oleracea

Cogan¹,

Harvey²,

Robinson³

et al. 2001

Plant Cell Rep

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The production of transgenic roots was scored for eight Brassica oleracea cultivars from broccoli, cabbage, cauliflower and kale following inoculation with an Agrobacterium rhizogenes cell line carrying a binary plasmid bearing the green fluorescence protein (gfp) gene in the T-DNA. Significant differences in the numbers of explants producing transgenic roots were observed between cultivars, ranging from 1.4% for Marathon F1 to 57.8% for the Green Duke F1. Three F1 cultivars were subjected to anther culture, and doubled-haploid (DH) lines were used for transformation. The DH lines produced showed considerable variation for transgenic root production with some lines showing increased efficiency compared to the parental F1 cultivar. Grouping of the DH lines into response classes with respect to transgenic root production allowed the development of potential genetic models to explain the variation in performance released from each F1 cultivar. No apparent segregation distortion for transgenic root production was observed in the DH lines following anther culture.

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“…In this investigation, susceptibility was not only an indicator of a genotype's ability to attract A. tumefaciens but also a measure of the uptake, integration and expression of the T-DNA. It should be noted, however, that crown gall formation and growth depend not only on the efficiency of gene transfer but also on the hormonal status of the plant (Puddephat et al 1996). Further work by our group has demonstrated a strong correlation between crown gall formation in DH lines and b-glucuoronidase expression (r=0.81, P<0.01) when the same genotypes were transformed with disarmed strains of A. tumefaciens (data not shown), suggesting that the genetic control of crown gall formation, as described here, relates to susceptibility to A. tumefaciens and not to the hormonal status of the plant (i.e.…”

Section: Discussionmentioning

confidence: 97%

Genetic analysis of Agrobacterium tumefaciens susceptibility in Brassica oleracea

et al. 2003

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The genetic control and heritability of Agrobacterium tumefaciens susceptibility was investigated using a doubled haploid (DH) mapping population of Brassica oleracea and the associated RFLP map. Preliminary studies were carried out by analysis of an 8 x 8 diallel, for which the parental lines were selected to include a range of susceptibilities to A. tumefaciens. The variation observed within the diallel was attributed to both additive and dominant gene effects, with additive gene effects being more important. A broad sense heritability value of 0.95 suggested that 95% of the observed variation was due to genetic effects, with just 5% attributed to non-genetic or environmental effects. A high narrow-sense heritability value of 0.79 suggested that 79% of this trait was controlled by additive gene effects and, therefore, the potential to introduce this trait into breeding material is high. Fifty-nine DH lines from the mapping population were screened for susceptibility towards A. tumefaciens. Variation in susceptibility was observed across the population. The results of the DH screen were entered into the mapping programme MAPQTL and a highly significant quantitative trait loci (QTL) associated with susceptibility to A. tumefaciens was identified on linkage group 09. The use of substitution lines covering this region confirmed the location of this QTL. This work shows that susceptibility to A. tumefaciens is a heritable trait, and the transfer of susceptibility into resistant lines is demonstrated. These findings may help to overcome genotype restrictions to genetic transformation.

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Transformation ofBrassica oleracea L.: a critical review

Cited by 56 publications

References 105 publications

Regeneration from leaf explants and protoplasts of Brassica oleracea var. botrytis (cauliflower)

Regeneration from leaf explants and protoplasts of Brassica oleracea var. botrytis (cauliflower)

The effects of anther culture and plant genetic background on Agrobacterium rhizogenes-mediated transformation of commercial cultivars and derived doubled-haploid Brassica oleracea

Genetic analysis of Agrobacterium tumefaciens susceptibility in Brassica oleracea

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