Transformation of two potato cultivars ‘Spunta’ and ‘Claustar’ (Solanum tuberosum) with lettuce mosaic virus coat protein gene and heterologous immunity to potato virus Y

Hassairi, Afif; Masmoudi, Khaled; Albouy, J.; Robaglia, Christophe; Jullien, Marc; Ellouz, Radhouane

doi:10.1016/s0168-9452(98)00078-8

Cited by 28 publications

(9 citation statements)

References 37 publications

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“…Hassairi et al (1998) observed a similar phenomenon in their study on the expression of the LMV Coat Protein coding gene by transgenic Solanum tuberosum plants.…”

Section: Western Blot Analysis In T1-generation Of a Thaliana Plantssupporting

confidence: 53%

Expression of a staphylokinase, a thrombolytic agent in Arabidopsis thaliana

Wiktorek-Smagur

Hnatuszko-Konka

Geszberg

et al. 2010

World J Microbiol Biotechnol

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A gene encoding staphylokinase from Staphylococcus aureus was cloned into the plant transformation binary vector pCAMBIA 1304. The transgene was introduced into the genome of A. thaliana via in planta Agrobacterium tumefaciens-mediated genetic transformation. The presence of the staphylokinase gene was confirmed by PCR in 60% of the investigated plants. The presence of the fusion protein (119 kDa) was confirmed by SDS-PAGE and Western blot analysis in protein extracts from putative transgenics. Furthermore, the amidolytic assay confirmed the activity of SAK in protein extracts in 23 out of 45 transgenic lines of A. thaliana plants.

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“…Hassairi et al (1998) observed a similar phenomenon in their study on the expression of the LMV Coat Protein coding gene by transgenic Solanum tuberosum plants.…”

Section: Western Blot Analysis In T1-generation Of a Thaliana Plantssupporting

confidence: 53%

Expression of a staphylokinase, a thrombolytic agent in Arabidopsis thaliana

Wiktorek-Smagur

Hnatuszko-Konka

Geszberg

et al. 2010

World J Microbiol Biotechnol

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“…Hassairi et al, 1998 Sense or antisense orientationObtained results indicate that transgenic potatoes with genetically improved resistance to PVY N are substantial and nutritional equivalence to the non-transgenic cultivar.Of the 800 transgenic tubers examined 5 months after harvesting the first field trial(1999/2000), 0.5% had potato tuber moth mine damage compared with 9% of non-transgenic tubers. After the second field trial (2000/2001) 0.06% of 5307 transgenic tubers had mine damage compared with damage to 25% of non-transgenic tubers Functional recLT-B can be produced in potato tubers, this recombinant protein is immunogenic and oral administration thereof elicits both systemic and local IgA responses in parentally primed, but not naive, animals.…”

mentioning

confidence: 96%

Potato: A Comparative Study of the Effect of Cultivars and Cultivation Conditions and Genetic Modification on the Physico-Chemical Properties of Potato Tubers in Conjunction with Multivariate Analysis Towards Authenticity

Arvanitoyannis¹,

Vaitsi²,

Mavromatis³

2008

Critical Reviews in Food Science and Nutrition

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Potato (Solanum tuberosum L.) is a highly nutritious, mild flavored, easy to blend food that has many possibilities for "building in" desired nutrients. Varietal and environmental differences are known to exist in the shape, size, and nutritional content of potatoes. Different populations opt for varying sensory properties in relation to their diets. Potatoes are a low energy food in comparison to cereals and legumes. The aim of this review was to present an update of the currently conducted studies both on the characterization of several potato varieties (physical, chemical, and sensory analysis) and by means of genetic modification. Towards this target, five comprehensive tables were compiled where all recent data (physicochemical properties) and GM varieties were presented in conjunction with multivariate analysis (chemometrics). The latter was shown to be effectively used towards authenticity purposes (identification of geographical origin, variety, GM).

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“…Most of these studies focused on resistance to different pest, virus, and fungal diseases (Davies 2002;Mullins et al 2006). Some examples include resistance to black scurf ( Rhizoctonia solani; Broglie et al 1991), Colorado potato beetle ( Leptinotarsa decemlineata) through expression of the Bacillus thuringiensis (Bt) toxin gene (Adang et al 1993;Perlak et al 1993), late blight (Cornelissen and Melchers 1993;Song et al 2003;Van der Vossen et al 2003, 2005Osusky et al 2004), potato leaf roll virus (PLRV), potato tuber moth ( Phthorimaea operculella; Davidson et al 2002), potato virus Y (PVY; Hassairi et al 1998), and soft rot ( Erwinia carotovora; During et al 1993). Examples of transgenic potato for increased nutritional characteristics include increase in total amino acid composition especially lysine, tyrosine, and sulphur-containing amino acids (Chakraborty et al 2000), selective increase in the essential amino acid methionine (Zeh et al 2001), increased inulin (Hellwege et al 2000), increased β-carotene and lutein (Ducreux et al 2005), and decrease in the glycoalkaloid solanine (Lukaszewicz et al 2004).…”

Section: Techniques For Potato Improvementmentioning

confidence: 99%