Transformation of tomato with the BADH gene from Atriplex improves salt tolerance

Jia, Gong-Xue; Zhu, Zhen; Chang, Fengqi; Li, Y.-X.

doi:10.1007/s00299-002-0489-1

Cited by 117 publications

(10 citation statements)

References 29 publications

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“…Plants respond to these stresses by modulating gene expression, which restores the cellular homeostasis, detoxification and recovery of growth [43,44]. For example, overexpression of betaine aldehyde dehydrogenase gene conferred salt stress tolerance in carrot, maize and tomato [45-47] and Zhang et al [48] showed that glycine betaine level is increased under drought, heat and salt stresses in jatropha also. When enzymes for glycine betaine biosynthesis are expressed in plants that do not naturally produce glycine betaine, they accumulate little glycine betaine, because their endogenous choline supply is inadequate [49,50].…”

Section: Resultsmentioning

confidence: 99%

Gene discovery from Jatropha curcas by sequencing of ESTs from normalized and full-length enriched cDNA library from developing seeds

et al. 2010

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BackgroundJatropha curcas L. is promoted as an important non-edible biodiesel crop worldwide. Jatropha oil, which is a triacylglycerol, can be directly blended with petro-diesel or transesterified with methanol and used as biodiesel. Genetic improvement in jatropha is needed to increase the seed yield, oil content, drought and pest resistance, and to modify oil composition so that it becomes a technically and economically preferred source for biodiesel production. However, genetic improvement efforts in jatropha could not take advantage of genetic engineering methods due to lack of cloned genes from this species. To overcome this hurdle, the current gene discovery project was initiated with an objective of isolating as many functional genes as possible from J. curcas by large scale sequencing of expressed sequence tags (ESTs).ResultsA normalized and full-length enriched cDNA library was constructed from developing seeds of J. curcas. The cDNA library contained about 1 × 106 clones and average insert size of the clones was 2.1 kb. Totally 12,084 ESTs were sequenced to average high quality read length of 576 bp. Contig analysis revealed 2258 contigs and 4751 singletons. Contig size ranged from 2-23 and there were 7333 ESTs in the contigs. This resulted in 7009 unigenes which were annotated by BLASTX. It showed 3982 unigenes with significant similarity to known genes and 2836 unigenes with significant similarity to genes of unknown, hypothetical and putative proteins. The remaining 191 unigenes which did not show similarity with any genes in the public database may encode for unique genes. Functional classification revealed unigenes related to broad range of cellular, molecular and biological functions. Among the 7009 unigenes, 6233 unigenes were identified to be potential full-length genes.ConclusionsThe high quality normalized cDNA library was constructed from developing seeds of J. curcas for the first time and 7009 unigenes coding for diverse biological functions including oil biosynthesis were identified. These genes will serve as invaluable genetic resource for crop improvement in jatropha to make it an ideal and profitable crop for biodiesel production.

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Section: Resultsmentioning

confidence: 99%

Gene discovery from Jatropha curcas by sequencing of ESTs from normalized and full-length enriched cDNA library from developing seeds

et al. 2010

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“…A choline monooxygenase gene isolated from A. hortensis ( AhCMO ) has been used for glycinebetaine production in tobacco [16] and cotton plants [17] to improve their abiotic stress tolerance. A betaine aldehyde dehydrogenase gene from A. hortensis ( AhBADH ) was introduced into tomato and trifoliate orange [18,19] and significantly improved salt tolerance in transgenic plants during growth. In transgenic tobacco, AhDREB1 isolated from A. hortensis led to accumulation of its putative downstream genes and exhibited increasing stress tolerance [20].…”

Section: Introductionmentioning

confidence: 99%

Generation and Analysis of Expressed Sequence Tags (ESTs) from Halophyte Atriplex canescens to Explore Salt-Responsive Related Genes

Li¹,

Yu²,

Chen³

et al. 2014

IJMS

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Little information is available on gene expression profiling of halophyte A. canescens. To elucidate the molecular mechanism for stress tolerance in A. canescens, a full-length complementary DNA library was generated from A. canescens exposed to 400 mM NaCl, and provided 343 high-quality ESTs. In an evaluation of 343 valid EST sequences in the cDNA library, 197 unigenes were assembled, among which 190 unigenes (83.1% ESTs) were identified according to their significant similarities with proteins of known functions. All the 343 EST sequences have been deposited in the dbEST GenBank under accession numbers JZ535802 to JZ536144. According to Arabidopsis MIPS functional category and GO classifications, we identified 193 unigenes of the 311 annotations EST, representing 72 non-redundant unigenes sharing similarities with genes related to the defense response. The sets of ESTs obtained provide a rich genetic resource and 17 up-regulated genes related to salt stress resistance were identified by qRT-PCR. Six of these genes may contribute crucially to earlier and later stage salt stress resistance. Additionally, among the 343 unigenes sequences, 22 simple sequence repeats (SSRs) were also identified contributing to the study of A. canescens resources.

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“…In the present study, GA content was significantly reduced and seed germination time was delayed by salt. ABA is involved in responses to environmental stress such as salinity (Jia et al, 2002), and is required by the plant for stress tolerance (Hamayun et al, 2010a). Umezawa et al (2001) found that the leaf ABA content in Lee (salt-tolerant) increased significantly under salt stress, while that in Enrei (salt-sensitive) showed only a slight increase.…”

Section: Phytohormonesmentioning

confidence: 99%

Proteomic analysis of seed germination under salt stress in soybeans

Fan

Zheng

et al. 2011

J. Zhejiang Univ. Sci. B

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Soybean (Glycine max (L.) Merrill) is a salt-sensitive crop, and its production is severely affected by saline soils. Therefore, the response of soybean seeds to salt stress during germination was investigated at both physiological and proteomic levels. The salt-tolerant cultivar Lee68 and salt-sensitive cultivar N2899 were exposed to 100 mmol/L NaCl until radicle protrusion from the seed coat. In both cultivars, the final germination percentage was not affected by salt, but the mean germination times of Lee68 and N2899 were delayed by 0.3 and 1.0 d, respectively, compared with controls. In response to salt stress, the abscisic acid content increased, and gibberellic acid (GA 1+3 ) and isopentenyladenosine decreased. Indole-3-acetic acid increased in Lee68, but remained unchanged in N2899. The proteins extracted from germinated seeds were separated using two-dimensional gel electrophoresis (2-DE), followed by Coomassie brilliant blue G-250 staining. About 350 protein spots from 2-DE gels of pH range 3 to 10 and 650 spots from gels of pH range 4 to 7 were reproducibly resolved, of which 18 protein spots showed changes in abundance as a result of salt stress in both cultivars. After matrix-assisted laser desorption ionization-time of flight-mass spectroscopy (MALDI-TOF-MS) analysis of the differentially expressed proteins, the peptide mass fingerprint was searched against the soybean UniGene database and nine proteins were successfully identified. Ferritin and 20S proteasome subunit β-6 were up-regulated in both cultivars. Glyceraldehyde 3-phosphate dehydrogenase, glutathione S-transferase (GST) 9, GST 10, and seed maturation protein PM36 were down-regulated in Lee68 by salt, but still remained at a certain level. However, these proteins were present in lower levels in control N2899 and were up-regulated under salt stress. The results indicate that these proteins might have important roles in defense mechanisms against salt stress during soybean seed germination.

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Transformation of tomato with the BADH gene from Atriplex improves salt tolerance

Cited by 117 publications

References 29 publications

Gene discovery from Jatropha curcas by sequencing of ESTs from normalized and full-length enriched cDNA library from developing seeds

Gene discovery from Jatropha curcas by sequencing of ESTs from normalized and full-length enriched cDNA library from developing seeds

Generation and Analysis of Expressed Sequence Tags (ESTs) from Halophyte Atriplex canescens to Explore Salt-Responsive Related Genes

Proteomic analysis of seed germination under salt stress in soybeans

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