Transformation of the gram-positive bacterium Clavibacter xyli subsp. cynodontis by electroporation with plasmids from the IncP incompatibility group

Metzler, M. C.; Zhang, Yongping; Chen, Tseh-An

doi:10.1128/jb.174.13.4500-4503.1992

Cited by 40 publications

(37 citation statements)

References 21 publications

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“…The parA gene of the pCXC100 par locus encodes a typical Walker-type ATPase, indicating that pCXC100 may employ an active partition mechanism to stabilize itself in host cells, consistent with the fact that this cryptic plasmid is a low-copy- (26,29). The parB gene is typically located downstream of parA in the active partitioning operon.…”

Section: Resultsmentioning

confidence: 94%

“…L. xyli subsp. cynodontis was grown on solid medium (DM agar) at 28°C as previously described (29). Antibiotics for selection of resistant E. coli cells were added to final concentrations of 60 g/ml of ampicillin, 25 g/ml of chloramphenicol, and 10 g/ml of tetracycline.…”

Section: Methodsmentioning

confidence: 99%

“…cynodontis strain no. 3, lacking the 51-kb native plasmid, was transformed by plasmid containing derivatives of the pCXC100 replicon by use of an electroporation method (29).…”

Section: Methodsmentioning

confidence: 99%

“…A cryptic plasmid about 51 kb in size, namely, pCXC100, was harbored by some L. xyli subsp. cynodontis isolates but not by others (29). The 5-kb replicon of pCXC100, in which a parA homologue and a downstream open reading frame (orf4) that has no homologues with any ParB sequence are present, has been identified previously (26).…”

mentioning

confidence: 99%

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A Type Ib ParB Protein Involved in Plasmid Partitioning in a Gram-Positive Bacterium

Yin

Xie

et al. 2006

J Bacteriol

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Our current understanding of segregation of prokaryotic plasmids has been derived mainly from the study of the gram-negative bacterial plasmids. We previously reported a replicon of the cryptic plasmid from a gram-positive bacterium, Leifsonia xyli subsp. cynodontis. The replicon contains a putative plasmid partition cassette including a Walker-type ATPase followed by open reading frame 4 without sequence homologue. Here we reported that the orf4 gene was essential for maintaining the plasmid stability in L. xyli subsp. cynodontis. Furthermore, the purified orf4 protein specifically and cooperatively bound to direct repeat sequences located upstream of the parA gene in vitro, indicating that orf4 is a parB gene and that the direct repeat DNA sequences constitute a partition site, parS. The location of parS and the features of ParA and ParB proteins suggest that this plasmid partition cassette belongs to type Ib, representing the first type Ib cassette identified from a gram-positive bacterial plasmid.

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Section: Resultsmentioning

confidence: 94%

Section: Methodsmentioning

confidence: 99%

“…cynodontis strain no. 3, lacking the 51-kb native plasmid, was transformed by plasmid containing derivatives of the pCXC100 replicon by use of an electroporation method (29).…”

Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 99%

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A Type Ib ParB Protein Involved in Plasmid Partitioning in a Gram-Positive Bacterium

Yin

Xie

et al. 2006

J Bacteriol

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“…cynodontis (269,292,431). Information on pCXC100 is limited: the RepAencoding protein is homologous to that of the plasmids in the mycobacterial pLR7 family, and a region associated with plasmid stability comprises parA, which is necessary for plasmid resolution, and a gene encoding a hypothetical protein (269).…”

Section: Extrachromosomal Dna Elements In Leifsoniamentioning

confidence: 99%

Genomics ofActinobacteria: Tracing the Evolutionary History of an Ancient Phylum

Ventura

Canchaya

Tauch

et al. 2007

Microbiol Mol Biol Rev

930

638

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SUMMARY Actinobacteria constitute one of the largest phyla among Bacteria and represent gram-positive bacteria with a high G+C content in their DNA. This bacterial group includes microorganisms exhibiting a wide spectrum of morphologies, from coccoid to fragmenting hyphal forms, as well as possessing highly variable physiological and metabolic properties. Furthermore, Actinobacteria members have adopted different lifestyles, and can be pathogens (e.g., Corynebacterium, Mycobacterium, Nocardia, Tropheryma, and Propionibacterium), soil inhabitants (Streptomyces), plant commensals (Leifsonia), or gastrointestinal commensals (Bifidobacterium). The divergence of Actinobacteria from other bacteria is ancient, making it impossible to identify the phylogenetically closest bacterial group to Actinobacteria. Genome sequence analysis has revolutionized every aspect of bacterial biology by enhancing the understanding of the genetics, physiology, and evolutionary development of bacteria. Various actinobacterial genomes have been sequenced, revealing a wide genomic heterogeneity probably as a reflection of their biodiversity. This review provides an account of the recent explosion of actinobacterial genomics data and an attempt to place this in a biological and evolutionary context.

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Functional Screening a Wide Host‐Range Metagenomic Library from a Wastewater Treatment Plant Yields a Novel Alcohol/Aldehyde Dehydrogenase

Wexler

Bond

Richardson

et al. 2011

Handbook of Molecular Microbial Ecology II

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Transformation of the gram-positive bacterium Clavibacter xyli subsp. cynodontis by electroporation with plasmids from the IncP incompatibility group

Cited by 40 publications

References 21 publications

A Type Ib ParB Protein Involved in Plasmid Partitioning in a Gram-Positive Bacterium

A Type Ib ParB Protein Involved in Plasmid Partitioning in a Gram-Positive Bacterium

Genomics ofActinobacteria: Tracing the Evolutionary History of an Ancient Phylum

Functional Screening a Wide Host‐Range Metagenomic Library from a Wastewater Treatment Plant Yields a Novel Alcohol/Aldehyde Dehydrogenase

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