Transformation of Rodent Lymphoid Cells by an Encephalitogen of Human Origin

Dau, Peter C.; Peterson, Reece L.

doi:10.1159/000230188

Cited by 33 publications

(7 citation statements)

References 12 publications

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“…According t o the data presented here, lymphocytes sensitized to either BE or Cop 1 :an be detected in the lymph nodes of guinea pigs and in lymph nodes and spleen of rabbits. It has been reported that BE-sensitized cells can be demonstrated in homologous reaction by means of thymidine incorporation in spleens of guinea pigs [21] and in peripheral blood lymphocytes of rabbits [22]. However, we have found that both in guinea pigs and in rabbit: BE-sensitized cells appear preferentially in the draining lymph nodes, rather than in the spleen.…”

Section: Discussioncontrasting

confidence: 70%

In vivo and in vitro immunological cross‐reactions between basic encephalitogen and synthetic basic polypeptides capable of suppressing experimental allergic encephalomyelitis

et al. 1973

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A significant extent of immunological cross‐reactivity has been demonstrated between the basic encephalitogenic protein of bovine origin and several synthetic amino acid copolymers which have suppressive effect on experimental allergic encephalomyelitis (EAE). This cross‐reactivity has been conclusively established on the cellular level, both in vivo by means of delayed hypersensitivity skin tests and in vitro using transformation of sensitized lymphocytes, as measured by incorporation of radioactive thymidine. The in vitro experiments have been conducted with lymph node cells from guinea pigs of both random bred and inbred strains, and on spleen and lymph node cells from rabbits. Definite cross‐reactivity was observed between the basic encephalitogen and all the synthetic copolymers which were previously shown effective in suppression of EAE, whereas ineffective copolymers or unrelated proteins did not show any cross‐reactivity. In the case of strain 2 guinea pigs and rabbit lymph node cells the cross‐reactivity in vitro was manifested by direct cross‐stimulation of the lymphocytes, whereas in random‐bred or strain 13 guinea pigs and rabbit spleen cells, the cross‐reaction was detected only by means of specific inhibition of the homologous stimulation by the heterologous antigen. A limited extent of cross‐reactivity was observed on the humoral level as well; antibodies provoked in guinea pigs against the synthetic copolymer Cop 1 cross‐reacted in the passive cutaneous anaphylaxis assay with the bovine basic encephalitogen.

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Section: Discussioncontrasting

confidence: 70%

In vivo and in vitro immunological cross‐reactions between basic encephalitogen and synthetic basic polypeptides capable of suppressing experimental allergic encephalomyelitis

et al. 1973

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“…The first of these was the demonstration in 1965 by David and Paterson (1965) that the migration from capillary tubes of peritoneal exudates from guinea pigs sensitized with homologous spinal cord antigens could be inhibited by the sensitizing extracL This observation was confirmed using purified basic protein as the sensitizing antigen (Hughes and Field, 1968;Brockman et al, 1968). In addition to studies with macrophage migration inhibition, other in vitra correlates of cell-mediated immunity have included study of the Iymphocyte proliferative response (Dau and Peterson, 1969) and Iymphotoxin production (Ellison et al, 1971). All these have been demonstrated using the basic protein.…”

Section: Evidence For Cell-mediated Response In Eaementioning

confidence: 99%

Myelin

1984

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“…These include macrophage migration inhibition, lymphocyte proliferation, and Iymphotoxin production , Dau & Peterson 1969, Rauch et al 1969, Bergstrand & Kallen 1971, Ellison et al 1971, Lisak & Zweiman 1973. These include macrophage migration inhibition, lymphocyte proliferation, and Iymphotoxin production , Dau & Peterson 1969, Rauch et al 1969, Bergstrand & Kallen 1971, Ellison et al 1971, Lisak & Zweiman 1973.…”

Section: Cell-mediated Immunity To Myelin Basic Protein In Animal Andmentioning

confidence: 99%